A rapid method for isolation of total DNA from pathogenic filamentous plant fungi
Authors: D. GonzÃ?¡lez-Mendoza, R. Argumedo-Delira, A. Morales-Trejo, A. Pulido-Herrera, L. Cervantes-DÃ?Âaz, O. Grimaldo-Juarez and A. AlarcÃ?³n
DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terre.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr680
- DOI:
- 10.4238/vol9-1gmr680
CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study
Authors: Y.L. Song, L. Wang, J.C. Ren and Z.H. Xu
To date, no study has investigated the association between CYP1A2-163C/A polymorphism and bladder cancer risk in a Chinese population. Here, we extracted genomic DNA from peripheral white blood cells, and differentiated CYP1A2 alleles by polymerase chain reaction-based restriction fragment length polymorphism methods. Differen.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026298
- DOI:
- 10.4238/gmr.15026298
Relationship between NRAMP1 gene polymorphism and efficacy of BCG vaccine in a helminth-infected population
Authors: A.A. Badawy, R.S. Yahya, S.I. Awad, G.A. Al-Sawah and N.A. Kizilbash
Infection of mothers with schistosomiasis and filariasis has been shown to influence infant responses to neonatal Bacille Calmette-Guérin (BCG) immunization. The genetic makeup of infants is also considered an important determinant for the activity of BCG vaccine. The effect of natural resistance-associated macrophage protein 1 (NRAMP1) gene polymo.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.30.4
- DOI:
- 10.4238/2013.January.30.4
Analysis of allelic variants of rdxA associated with metronidazole resistance in Helicobacter pylori: detection of common genotypes in rdxA by multiplex allele-specific polymerase chain reaction
Authors: T.R.P. Butlop, N.T.N. Mungkote and N.T.R. Chaichanawongsaroj
Resistance to metronidazole (Mtz) in Helicobacter pylori is a major problem worldwide, especially in developing countries. Alterations in Mtz nitroreductase enzymes, such as oxygen-insensitive NADPH nitroreductase (RdxA) and NADPH flavin oxidoreductase (FrxA), are the major contributing factors for this resistance. In this stu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038674
- DOI:
- 10.4238/gmr.15038674
Caspase-3/-8/-9, Bax and Bcl-2 expression in the cerebellum, lymph nodes and leukocytes of dogs naturally infected with canine distemper virus
Authors: H.L. Del Puerto, A.S. Martins, L. Moro, A. Milsted, F. Alves, G.F. Braz and A.C. Vasconcelos
Canine distemper is an immunosuppressive disease caused by the canine distemper virus (CDV). Pathogenesis mainly involves the central nervous system and immunosuppression. Dogs naturally infected with CDV develop apoptotic cells in lymphoid tissues and the cerebellum, but this apoptotic mechanism is not well characterized. To .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr717
- DOI:
- 10.4238/vol9-1gmr717
A literature-based systematic HuGE review and meta-analysis show that CASP gene family polymorphisms are associated with risk of lung cancer
Authors: Z.Y. Zhang, Y. Xuan, X.Y. Jin, X. Tian and R. Wu
The caspase (CASP) gene family is known to be involved in apoptosis, cytokine maturation, cell growth, and differentiation. A large number of single nucleotide polymorphisms (SNPs) in the CASP gene family have been increasingly recognized as important regulators in the development of lung cancer. However, this specific association is still controversial. .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.22
- DOI:
- 10.4238/2013.January.4.22
Genetic variability of ERCC1 and ERCC2 genes involved in the nucleotide excision repair pathway influences the treatment outcome of gastric cancer
Authors: D.L. Zheng, G.D. Tang, Y.N. Chen, T. Zhang and M.B. Qin
We conducted a prospective study to investigate whether ERCC1 rs11615 and rs3212986 and ERCC2 rs13181 and rs1799793 gene polymorphisms could serve as potential biomarkers for the prognosis of gastric cancer. Between January 2010 and December 2012, 246 patients with pathologically proven gastric cancer who were receiving platin.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027384
- DOI:
- 10.4238/gmr.15027384
Chemical and genetic diversity of high-seed-yield sorghum (Sorghum bicolor M.) germplasms
Authors: J. Ryu, S.B. Im, S.J. Kwon, J.W. Ahn, S.W. Jeong and S.Y. Kang
This study evaluated the chemical and genetic diversity of high-seed-yield sorghum germplasms from Korea, the United States, and South Africa. We identified significant differences in the chemical contents of whole plants at the heading stage in all cultivars, including differences in crude protein, fat, fiber, ash, neutral de.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038677
- DOI:
- 10.4238/gmr.15038677
Polymorphisms and allele frequencies of glutathione S-transferases A1 and P1 genes in the Polish population
Authors: M. Skrzypczak-Zielinska, O. Zakerska-Banaszak, B. Tamowicz,I. Sobieraj, N. Drweska-Matelska, M. Szalata, R. Slomski and A. Mikstacki
Glutathione S-transferases (GST) A1 and P1 are crucial enzymes involved in the biotransformation of drugs, carcinogens, and toxins, and their activity may influence drug response, susceptibility to diseases, and carcinogenesis. The genes encoding these enzymes, GSTA1 and GSTP1, have been examined in many studies because of their genetic variability, which.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.31.15
- DOI:
- 10.4238/2015.March.31.15
Genetic diversity of the endangered scaly-sided merganser (Mergus squamatus) in the wintering habitat of central-southern China
Authors: Z.-Q. Zhang, C.-J. Qin, D.-A. Fang, A.-P. Wang and C.-H. Luo and K. Liu
The scaly-sided merganser (Mergus squamatus), found in temperate East Asia, has been reduced to a very small population. Central and southern China are its main wintering habitat. However, populations have declined greatly since the 1980s due to habitat loss and degradation, and poaching. To meet the urgent need for up-to-date conservation information, we.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.April.26.2
- DOI:
- 10.4238/2013.April.26.2