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Human Genetics   Research Article

Genetic diversity of the Arctic fox using SRAP markers

Authors: M. Zhang1 and X.J. Bai2

Sequence-related amplified polymorphism (SRAP) is a recently developed molecular marker technique that is stable, simple, reliable, and achieves moderate to high numbers of codominant markers. This study is the first to apply SRAP markers in a mammal, namely the Arctic fox. In order to investigate the genetic diversity of the .. Read More»

Genet. Mol. Res. 13(4):
2013.December.4.4
DOI:
10.4238/2013.December.4.4
Medical Genetics   Research Article

Clinical application of carbon nanoparticle lymph node tracer in the VI region lymph node dissection of differentiated thyroid cancer

Authors: S.P. Sun, Y. Zhang, Z.Q. Cui, Q. Chen, W. Zhang, C.X. Zhou, P.P. Xie and B.G. Liu

The application and clinical significance of carbon nanoparticle lymph tracer in the VI region (central region) lymph node dissection of differentiated thyroid cancer was investigated. Eighty patients with differentiated thyroid cancer were equally divided into the carbon nanoparticle-marked group (ipsilateral thyroid injectio.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.April.30.4
DOI:
http://dx.doi.org/10.4238/2014.April.30.4
Animal Genetics   Research Article

Expression of calpastatin and myostatin genes associated with lamb meat quality

Authors: A. Bagatoli1, E. Gasparino1, M.A.M. Soares2, R.M. Amaral1,F.A.F. Macedo1, D.M. Voltolini1 and A.P. Del Vesco1

Calpastatin (CAST) is an endogenous calpain inhibitor and its main function is to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration. The myostatin gene (GDF-8) acts as a negative regulator of skeletal muscle growth. The expression of these two genes, as well as their interaction, affects the quality of the meat.. Read More»

Genet. Mol. Res. 13(4):
2013.December.4.3
DOI:
10.4238/2013.December.4.3
Animal Genetics   Research Article

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

Authors: Y.X. Liu, G.Q. Shi, H.X. Wang, P.C. Wan, H. Tang, H. Yang and F. Guan

High glycine-tyrosine proteins (HGTPs), also known as keratin-associated proteins (KAPs), play a key role in the major structures and mechanical properties of wool fiber. Sheep HGTPs consist of three multigene families: KAP6, KAP7, and KAP8 genes. Polymorphisms of these three genes have been proposed to have important effects .. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.April.30.5
DOI:
http://dx.doi.org/10.4238/2014.April.30.5
Animal Genetics   Research Article

Cross-amplification of nonspecific micro-satellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer

Authors: J.C. Mar�­n, P. Orozco-terWengel, K. Romero, J.P. V�¡squez, V. Varas and J.A. Vianna

Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully i.. Read More»

Genet. Mol. Res. 13(2):
2014.April.25.4
DOI:
10.4238/2014.April.25.4
Plant Genetics   Research Article

Functions of microRNA in response to cocaine stimulation

Authors: L.-F. Xu, J. Wang, F.B. Lv and Q. Song

MicroRNAs (miRNAs) are a type of non-protein-coding single-stranded RNA, which are typically 20-25 nt in length. miRNAs play important roles in various biological processes, including development, cell proliferation, differentiation, and apoptosis. We aimed to detect the miRNA response to cocaine stimulations and their target .. Read More»

Genet. Mol. Res. 13(4):
2013.December.4.2
DOI:
10.4238/2013.December.4.2
Plant Genetics   Research Article

Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco

Authors: L.L. Li, H. Cheng, H.H. Yuan, F. Xu, S.Y. Cheng and F.L. Cao

The regulative sequence (2273 bp) of the chalcone synthase gene promoter of biloba was cloned by genomic walking. A 2273-bp promoter 5' upstream translation start site of GbCHS was cloned and designated as GbCHSP. pBI121+CHSP:GUS and pBI121-35S:GUS were constructed and transformed into tobacco by LBA4404. We found that GbCHSP .. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.April.30.6
DOI:
http://dx.doi.org/10.4238/2014.April.30.6
Animal Genetics   Research Article

Isolation and characterization of novel microsatellite loci in Nibea albiflora

Authors: D.D. Xu, B. Lou, S.L. Li, Y.R. Zhang, J. Xin and W. Zhan

Eleven novel microsatellite loci were isolated from a (CA)10-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected heterozy.. Read More»

Genet. Mol. Res. 13(4):
2013.December.4.1
DOI:
10.4238/2013.December.4.1
Human Genetics   Research Article

Determination of the genetic diversity among accessions of Senna spectabilis (canaf�­stula) by using RAPD markers

Authors: M.F. Santos1, R.B. Ara�ºjo Neto2, M.P.S.B.C. Nascimento2 and P.S.C. Lima2

Senna spectabilis (DC.) H.S. Irwin & Barneby (Fabaceae; Caesalpinioideae), commonly known as “canafístula” or “cassia”, is widely used in the semi-arid region of northeastern Brazil as a source of forage and timber. The plant presents a high nutritional content in comparison with other forage species that are native to t.. Read More»

Genet. Mol. Res. 13(4):
2013.December.2.12
DOI:
10.4238/2013.December.2.12
Plant Genetics   Research Article

Distribution and pathogen identification of cassava brown leaf spot in China

Authors: Y.L. Pei, T. Shi, C.P. Li, X.B. Liu, J.M. Cai and G.X. Huang

 Cassava brown leaf spot surveys were conducted in the main cassava plantation areas of China between 2007 and 2012 in order to understand the distribution of the disease. Cassava plants were damaged by the disease to different degrees in most of the survey sites. Samples were collected and seven strains were isolated fro.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.April.30.7
DOI:
http://dx.doi.org/10.4238/2014.April.30.7