Impact of caffeine on �² cell proliferation and apoptosis under the influence of palmitic acid
Authors: L. Chen, M. Yu, T. Shen, J. Xia and B.L. Xu
We examined the influence of caffeine on the proliferation and apoptosis of b cells cultured in vitro in the presence of the free fatty acid palmitic acid (PA). Different concentrations of caffeine (1-100 mM) and free fatty PA were added to cultured b cells. The MTT assay was used to analyze cell proliferative activity; flow cytometry was used to measure .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.4
- DOI:
- 10.4238/2015.May.29.4
Investigation of the role of VEGF gene polymorphisms in the risk of osteosarcoma
Authors: G. Zhang, R. Bai, T. Zhang, H. Zhang, S.Z. Wen and D.M. Jiang
We conducted a case-control study in a Chinese population, and assessed whether the VEGF -634G/C, +936C/T, and +1612G/A polymorphisms could affect the risk of osteosarcoma and its association with environmental factors. A total of 180 consecutive osteosarcoma patients and 360 controls were included in our study. The genotypes of VEGF -634G/C, +936C/T, and.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.16
- DOI:
- 10.4238/2015.July.27.16
Screening relevant genes of tolerance to low phosphorus in maize using cDNA-amplified fragment length polymorphism
Authors: H.Y. Jiang, Z. Li, J. Zhao, Q. Ma, B.J. Cheng and S.W. Zhu
Soil contains a large amount of phosphorus, but plants cannot absorb most of this phosphorus effectively. Low inorganic phosphorus has been singled out as a major constraint that leads to a perpetually low Zea mays (maize) grain yield. The fundamental approach to solving this problem is to screen new genes of low phosphorous (LP) tolerance. Consequently, .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.5
- DOI:
- 10.4238/2015.May.29.5
A protocol for high-quality genomic DNA extraction from legumes
Authors: I.O. Agbagwa S. Datta P.G. Patil P. Singh N. Nadarajan
Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding li.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.14.1
- DOI:
- 10.4238/2012.September.14.1
PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells
Authors: C.-G. Sun, J. Zhuang, W.-J. Teng, Z. Wang and S.-S. Du
We explored whether p53 upregulated modulator of apoptosis (PUMA) gene transfection could enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells. The liposome-mediated recombinant eukaryotic expression vector PUMA-pCDNA3 and empty vector plasmid were stably transfected into MCF-7 cells. Epirubicin (0.01-100 μM) was applied.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.6
- DOI:
- 10.4238/2015.May.29.6
Molecular cloning and characterization of a novel Y-box gene from Sepiella maindroni
Authors: H.P. Si, C.L.Wang, Y.Y. Zhang, C.K. Mu, P.P. Zhan, R.H. Li and W.W. Song
Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.7
- DOI:
- 10.4238/2015.May.29.7
Characterization of hemocyanin from the mud crab Scylla paramamosain and its expression analysis in different tissues, at various stages, and under Vibrio parahaemolyticus infection
Authors: J. Wang, F.Y. Zhang, W. Song, Y.B. Fang, J.H. Hu, M. Zhao, K.J. Jiang and L.B. Ma
Hemocyanin is an important respiratory protein in many arthropod and mollusk species. Here, four cDNAs (SpHc1, SpHc2, SpHc3, and SpHc4), encoding distinct hemocyanin subunits from Scylla paramamosain were cloned using EST analyses and the rapid amplification of cDNA ends. The four full-length cDNA fragments (SpHc1-4) were 2281, 2002, 2184, and 2069 bp, re.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Synergistic and threshold effects of GH1 and GHR promoter size variation on body growth and fat accrual in young Nelore (Bos indicus) bulls
Authors: M.A. Dani, M. von Cube, I.L. Freire, L. Suguisawa, C. Fischer, S.U. Dani
A synergistic effect in the somatotropic axis (GH1-GHR-IGF1) was observed in 736 young Nelore (Bos indicus) bulls under ad libitum grass feeding conditions on irrigated pasture in central Brazil. Stepwise substitution of shorter alleles of the promoter region of the growth hormone gene (GH1) and the P1 promoter of the GH1 rece.. Read More»
- Genet. Mol. Res. 11(2):
- http://dx.doi.org/2012.June.29.11
- DOI:
- http://dx.doi.org/10.4238/2012.June.29.11
Genetic potential of common bean progenies selected for crude fiber content obtained through different breeding methods
Authors: V.A.P. J�ºnior, P.G.S. Melo, H.S. Pereira, P.Z. Bassinello and L.C. Melo
Gastrointestinal health is of great importance due to the increasing consumption of functional foods, especially those concerning diets rich in fiber content. The common bean has been valorized as a nutritious food due to its appreciable fiber content and the fact that it is consumed in many countries. The current study aimed to evaluate and compare the g.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.8
- DOI:
- 10.4238/2015.May.29.8
Entoepidemiology of Chagas disease in the Western region of the State of S�£o Paulo from 2004 to 2008, and cytogenetic analysis in Rhodnius neglectus (Hemiptera, Triatominae)
Authors: K.C.C. Alevi, L.A.C Rodas, E. Tartarotti, M.T.V. Azeredo-Oliveira and M.M. Guirado
To complement the epidemiological data and assist in the prophylaxis of Chagas disease in the State of São Paulo, we examined entomological lifting conducted in 40 municipalities of the Western region of the state from 2004 to 2008, highlighted the main vector species in this region, and reanalyzed the cytogenetic characteristics of Rhodnius neglec.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.9
- DOI:
- 10.4238/2015.May.29.9