Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice
Authors: T.D.A. Costa, S. Vieira, S.F. Andrade and E.L. Maistro
Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr475
- DOI:
- 10.4238/vol7-3gmr475
Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species
Authors: E.M. Gatphoh, S.K. Sharma, K. Rajkumari and S. Rama Rao
The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, bo.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1083
- DOI:
- 10.4238/vol10-3gmr1083
Isolation and characterization of microsatellite loci from an endangered tree species, Toona ciliata var. pubescens
Authors: J. Liu Z.-X. Sun Y.-T. Chen J.-M. Jiang
Toona ciliata var. pubescens is considered an endangered tree species native to China. In order to help develop a conservation program for this species, we evaluated its genetic diversity and population genetics. We isolated microsatellite DNA loci using streptavidin beads. A genomic library, enriched with microsatellites, was constructed and screened by .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.19.4
- DOI:
- 10.4238/2012.September.19.4
Genetic linkage analysis of oral lichen planus in a Chinese family
Authors: Z. Wang, H. Yao, B. Cui, G. Ning and G.Y. Tang
Oral lichen planus (OLP) is a common oral inflammatory disease affecting about 1-2% of the general adult population. As with European families who are diagnosed with OLP, the Chinese family who we studied was diagnosed with a severe form of oral reticular and erosive lesions; moreover, two of the five affected individuals deve.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1137
- DOI:
- 10.4238/vol10-3gmr1137
Expression of IL-1, IL-6, TNF-�±, and iNOS in pregnant women with periodontal disease
Authors: C.C.M. Otenio I. Fonseca M.F. Martins L.C. Ribeiro N.M.S.P. Assis A.P. Ferreira R.A. Ribeiro
Periodontal disease is one of the most prevalent oral diseases. An association between this disease and pregnancy has been suggested, but available findings are controversial. We evaluated the expression levels of interleukins (IL-1β and IL-6), tumor necrosis factor-alpha (TNF-α), and inducible nitric oxide synthase (iNOS) in pregnant women wit.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.20.3
- DOI:
- 10.4238/2012.September.20.3
Study of closely related species within the Physalaemus cuvieri group (Anura): contribution of microsatellite markers
Authors: M. Conte, M.I. Zucchi, G.V. Andrade, A.P. Souza and S.M. Recco-Pimentel
Various species of the Physalaemus cuvieri group of frogs are difficult to distinguish morphologically, making molecular analysis an attractive alternative for indentifying members of this group, which is considered to be at risk because of loss of habitat. The genetic structure of natural populations of P. ephippifer and P. a.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1136
- DOI:
- 10.4238/vol10-3gmr1136
Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance
Authors: S. Ghanem
In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E. coli DH5a ΔrecA (recombi.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1334
- DOI:
- 10.4238/vol10-3gmr1334
Identification and expression analysis of multiple FRO gene copies in Medicago truncatula
Authors: Ma. del C. Orozco-Mosqueda G. Santoyo R. Farias-Rodriguez L. MacÃ?Âas-RodrÃ?Âguez E. Valencia-Cantero
Iron (Fe) is an essential element for plant growth. Commonly, this element is found in an oxidized form in soil, which is poorly available for plants. Therefore, plants have evolved ferric-chelate reductase enzymes (FRO) to reduce iron into a more soluble ferrous form. Fe scarcity in plants induce the FRO enzyme activity. Although the legume Medicago trun.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.9.7
- DOI:
- 10.4238/2012.October.9.7
Molecular cloning and characterization of two novel NAC genes from Mikania micrantha (Asteraceae)
Authors: D.M. Li J.H. Wang S.L. Peng G.F. Zhu F.B. LÃ?Â?
NAC proteins, which are plant-specific transcription factors, have been identified to play important roles in plant response to stresses and in plant development. The full-length cDNAs that encode 2 putative NAC proteins, designated as MmATAF1 and MmNAP, respectively, were cloned from Mikania micrantha by rapid amplification of cDNA ends. The full-length cDN.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.19.3
- DOI:
- 10.4238/2012.September.19.3
DNATagger, colors for codons
Authors: N.M. Scherer and D.M. Basso
DNATagger is a web-based tool for coloring and editing DNA, RNA and protein sequences and alignments. It is dedicated to the visualization of protein coding sequences and also protein sequence alignments to facilitate the comprehension of evolutionary processes in sequence analysis. The distinctive feature of DNATagger is the .. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3x-meeting003
- DOI:
- 10.4238/vol7-3x-meeting003