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Plant Genetics   Research Article

De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress

Authors: L.Q. Li, J. Li, Y. Chen, Y.F. Lu and L.M. Lu

Tobacco is an economically important crop, and its potassium content can greatly affect the quality of tobacco leaves. However, the molecular mechanism involved in potassium starvation in tobacco has not been elucidated to date. In this study, Illumina (Solexa) sequencing technology was used to analyze the transcriptome of tobacco seedlings under low-pota.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038599
DOI:
10.4238/gmr.15038599
Microbial Genetics   Research Article

Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant Acinetobacter spp

Authors: A.A. Carvalho, L.L. Cardoso, H.S. Nogueira, E.V. Menezes, M.A.S. Xavier, N.A.P. Barreto, L.F. Fernandes and A.R.E.O. Xavier

Acinetobacter sp isolates deserve special attention once they have emerged globally in healthcare institutions because they display numerous intrinsic and acquired drug-resistance mechanisms. This study assessed the antibiotic susceptibility profile, the presenceof the genetic marker blaOXA-23, and the clonal relationship among 34 nosocomial isolates of A.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038608
DOI:
10.4238/gmr.15038608
Animal Genetics   Research Article

Canine hepatozoonosis in southeastern Bahia, Brazil

Authors: T.V. Harvey, P.E.B. Guedes, T.N.A. Oliveira, M.S. AssunĂ?§Ă?£o, F.S. Carvalho, G.R. Albuquerque, F.L. Silva and R.S.A. Carlos

In Brazil, canine hepatozoonosis is a tick-borne subclinical hemoparasitosis caused by a protozoa Hepatozoon canis and is highly prevalent in dogs in rural areas. An epizootiological study was conducted to investigate the prevalence of H. canis in the canine population of Ituberá, Bahia, and to analyze any associated risk factors. Blood samples wer.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038623
DOI:
10.4238/gmr.15038623
Plant Genetics   Research Article

FSHR genotype affects estrogen levels but not pregnancy rates in Luxi cattle subjected to embryo transfer.

Authors: W.C. Yang, S.J. Li, L. Chen and L.G. Yang

Follicle-stimulating hormone receptor (FSHR), which mediates the functioning of FSH, plays a central role in reproduction. We investigated bovine FSHR gene polymorphisms and analyzed their relationships with pregnancy rates after embryo transfer and with hormone concentrations on the day of embryo transfer. One reported SNP of.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.March.12.8
DOI:
http://dx.doi.org/10.4238/2014.March.12.8
Animal Genetics   Research Article

Genetic association of sequence variation in exon 3 of the swine leukocyte antigen-DQA gene with piglet diarrhea in Large White, Landrace, and Duroc piglets

Authors: Q.L. Yang, X.Y. Huang, J.J. Kong, S.G. Zhao, L.X. Liu and S.B. Gun

Piglet diarrhea is one of the primary factors that affects the benefits of the swine industry. Recent studies have shown that exon 2 of the swine leukocyte antigen-DQA gene is associated with piglet resistance to diarrhea; however, the contributions of additional exon coding regions of this gene remain unclear. Here, we detected and sequenced variants in .. Read More»

Genet. Mol. Res. 15(3):
gmr.15038673
DOI:
10.4238/gmr.15038673
Human Genetics   Research Article

Prevalence of mutations in LEP, LEPR, and MC4R genes in individuals with severe obesity

Authors: B. Paolini, P.E. Maltese, I. Del Ciondolo, D. Tavian, S. Missaglia, C. Ciuoli, M. Zuntini, S. Cecchin, M. Bertelli and G. Pompucci

Obesity is a major public health concern; despite evidence of high heritability, the genetic causes of obesity remain unclear. In this study, we assessed the presence of mutations in three genes involved in the hypothalamic leptin-melanocortin regulation pathway (leptin, LEP; leptin receptor, LEPR; and melanocortin-4 receptor, MC4R), which is important fo.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038718
DOI:
10.4238/gmr.15038718
Plant Genetics   Research Article

Cloning and bioinformatic analysis of transcription factor MYB10 from the red-leaf peach

Authors: X.Q. Wang, J. Han, Y. Wen, W.B. Jiang, J.G. Fang, B.B. Zhang and R.J. Ma

In higher plants, the transcription factor MYB10 is an important regulator of anthocyanin biosynthesis. In order to study its role in the development of red coloration in peach leaves, the full-length MYB10 complementary DNA sequence of the red-leaf peach cultivar ‘Tsukuba No. 5’ (Prunus persica f. atropurpurea) wa.. Read More»

Genet. Mol. Res. 15(4):
gmr15048013
DOI:
10.4238/gmr15048013
Medical Genetics   Research Article

Characterization of the complete mitochondrial genome of Portunus pelagicus with implications for phylogenomics

Authors: C.Y. Ma, H.Y. Ma, G.J. Ren, W. Wang, W. Chen, J.X. Lu, X. Zou and L.B. Ma

This study determined the mitochondrial genome structure of the blue swimming crab (Portunus pelagicus), and elucidated its phylogenetic relationships among the species within the order Decapoda. The complete mitochondrial genome was 16,155 bp long, and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 DNA control regi.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038719
DOI:
10.4238/gmr.15038719
Animal Genetics   Research Article

Inclusion of cytoplasmic lineage effect and direct-maternal genetic covariance for genetic evaluation of growth traits in Nellore cattle

Authors: L. Grigoletto, E.C. Mattos, M.H.A. Santana, F. Baldi, J.P. Eler and J.B.S. Ferraz

We evaluated the impact of cytoplasmic lineage effects (Lc) for growth traits on genetic evaluation, including the genetic covariance between direct and maternal effects (sam). Pedigree data from 496,190 Nellore animals and observations on birth weight (BW, N = 243,391), weaning weight (WW, N = 431,681), and post-weaning weight gain adjusted to 345 days (.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038812
DOI:
10.4238/gmr.15038812
Animal Genetics   Research Article

Development of coding single nucleotide polymorphic markers in the pearl oyster Pinctada fucata based on next-generation sequencing and high-resolution melting analysis

Authors: S.G. Fan, J.F. Wei, Y.H. Guo, G.J. Huang and D.H. Yu

The pearl oyster Pinctada fucata is an important commercial marine shellfish that is cultured for producing saltwater pearls. In this study, 468 single nucleotide polymorphisms (SNPs) were screened from P. fucata transcriptome data, and 119 polymorphic SNPs were successfully isolated by a two-step small-amplicon high-resolutio.. Read More»

Genet. Mol. Res. 15(4):
gmr15049054
DOI:
10.4238/gmr15049054