Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi
Authors: B. Zeng, H.D. Yan, L.K. Huang, Y.C. Wang, J.H. Wu, X. Huang, A.L. Zhang, C.R. Wan2 and Q. Mu
Roegneria kamoji Ohwi is an excellent forage grass due to its high feeding value and high resistance to some biotic and abiotic stresses. However, the start codon targeted (SCoT) polymorphism has not been conducted on R. kamoji. In this study, an orthogonal L16 (45) design was employed to investigate the effects of five factor.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048968
- DOI:
- 10.4238/gmr15048968
Inhibition of Atg6 and Pi3K59F autophagy genes in neurons decreases lifespan and locomotor ability in Drosophila melanogaster
Authors: P.G. M�Angale and B.E. Staveley
Autophagy is a cellular mechanism implicated in the pathology of Parkinson’s disease. The proteins Atg6 (Beclin 1) and Pi3K59F are involved in autophagosome formation, a key step in the initiation of autophagy. We first used the GMR-Gal4 driver to determine the effect of reducing the expression of the genes encoding thes.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048953
- DOI:
- 10.4238/gmr15048953
Molecular cloning and immune response analysis of putative variable lipoproteins from Mycoplasma mycoides subsp capri
Authors: C.G. Xu, Y.Q. Hao, L. Zhang, R.X. Hao, X.L. Liu and Z.Y. Huang
Mycoplasma mycoides subsp capri is the cause of goat “MAKePS” (Mastitis, Arthritis, Keratoconjunctivitis, Pneumonia, Septicemia) syndrome. We identified three genes (GL_ 000459; 000461; 000462) as variable lipoprotein genes in the M. mycoides subsp capri str. PG3 genome by genomic information and comparative genomi.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.12.5
- DOI:
- http://dx.doi.org/10.4238/2014.March.12.5
Association of tumor necrosis factor-�± gene polymorphism with osteoarticular tuberculosis prognosis in a Hebei population
Authors: Y.J. Lv, S.J. Liu, W.N. Hu, G.P. Zhang, Q.Y. Ren, L.D. Zheng, Y.C. Zhang, R.Q. Li and Z.K. Zhang
This study investigated the association of tumor necrosis factor-α (TNF-α)-308, -238, and -863 polymorphisms with osteoarticular tuberculosis (OA-TB) prognosis in a Hebei population. Genomic DNA was extracted from venous blood samples of 120 OA-TB patients and 100 healthy volunteers. TNF-α-308, -238, and -863.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048937
- DOI:
- 10.4238/gmr15048937
Fine-scale genetic structure patterns in two freshwater fish species, Geophagus brasiliensis (Osteichthyes, Cichlidae) and Astyanax altiparanae (Osteichthyes, Characidae) throughout a Neotropical stream
Authors: D.G. Ferreira1, S.C. Lima3, W. Frantine-Silva3, J.F. Silva1, C. Apolin�¡rio-Silva3, S.H. Sofia3, S. Carvalho2 and B.A. Galindo1
Streams are very important environments for Neotropical freshwater fish fauna, and possess a high number of species. These small drainages are also highlighted by their intrinsic biological and physicochemical features; however, knowledge on the genetic distribution of fish in these drainages is limited. Therefore, in the pres.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048124
- DOI:
- 10.4238/gmr15048124
Effects of porcine circovirus type 2 and pseudorabies vaccine co-inoculation on regulatory cytokine mRNA expression in pig peripheral blood mononuclear cells
Authors: F. Gao, J.-L. Xie, C.-W. Jia, H.-Y. Ren and S.-H. Zhou
We investigated the immune response effects of porcine circovirus type 2 (PCV2) on cells inoculated with pseudorabies attenuated vaccine (PRV). Real-time PCR was used to detect the mRNA expression levels of the regulatory cytokines IL-4, IL-10, IL-12p40, and IFN-γ in pig peripheral blood mononuclear cells, after in vitro.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.12.6
- DOI:
- http://dx.doi.org/10.4238/2014.March.12.6
Overexpression of a glycine-rich protein gene in Lablab purpureus improves abiotic stress tolerance
Authors: L.M. Yao, Y.N. Jiang, X.X. Lu, B. Wang, P. Zhou and T.L. Wu
Glycine-rich protein (GRP) is involved in the response to abiotic and biotic stresses in plants. A novel GRP gene in Lablab purpureus has been identified. The cDNA of LpGRP was obtained from an SSH library constructed with root tissues of L. purpureus MEIDOU 2012 by waterholding for 10 days. The function of LpGRP was also eval.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048063
- DOI:
- 10.4238/gmr15048063
Expression and clinical significance of the obesity-related gene TNFAIP9 in obese children
Authors: L.Y. Gui, Y.L. Hu, Z.L. Hou and P. Wang
To investigate the expression of tumor necrosis factor-alpha inducible protein 9 (TNFAIP9) gene in obese children and its clinical significance, 36 simple obese children and 17 non-obese children were recruited as research subjects. The adipose tissue was obtained by abdominal operation. The expression of TNFAIP9 was detected using real-time fluorescence .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037995
- DOI:
- 10.4238/gmr.15037995
Sequence divergence of microsatellites for phylogeographic assessment of Moroccan Medicago species
Authors: N. Zitouna, S. Marghali, M. Gharbi, A. Haddioui and N. Trifi-Farah
Six Medicago species were investigated to characterize and valorize plant genetic resources of pastoral interest in Morocco. Samples were obtained from the core collection of the South Australian Research and Development Institute (SARDI). The transferability of single sequence repeat markers of Medicago truncatula was success.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.12.7
- DOI:
- http://dx.doi.org/10.4238/2014.March.12.7
Investigating genetic diversity in sapucaia using inter simple sequence repeat markers
Authors: R.C. Borges, F.M.G. Santos, M.C.C. Maia, P.S.C. Lima and S.E.S. Valente
Sapucaia is a tree species originating from the Brazilian Amazon and is widely distributed in Brazil, especially in the mid-north region (Piauí and Maranhão states). Its seeds are rich in calories and proteins, and possess great potential for commercialization. Little is known about the genetic variability in the germplasm of most Lecythis s.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038565
- DOI:
- 10.4238/gmr.15038565