Mining ORESTES no-match database: can we still contribute to cancer transcriptome?
Authors: Rog?©rio da Silva Fonseca, Dirce Maria Carraro and Helena Brentani
The Human Cancer Genome Project generated about 1 million expressed sequence tags by the ORESTES method, principally with the aim of obtaining data from cancer. Of this total, 341,680 showed no similarity with sequences in the public transcript databases, referred to as “no-match”. Some of them represent low abunda.. Read More»
- Genet. Mol. Res. 5(1):
Moquiniastrum polymorphum subsp floccosum extract: screening for mutagenic and antimutagenic activity
Authors: R.J. Oliveira, N. de David, J.R. Pesarini, T.D. Nogueira, C.A.L. Kassuya, R.L.B. Strapasson, M.E.A. Stefanello, A.C.D. Monreal, R. Matuo and A.C.M.B. Antoniolli-Silva
Moquiniastrum polymorphum subsp floccosum (Cabrera) G. Sancho is used in traditional Brazilian medicine to treat inflammation and infection, which is supported by scientific data. However, only one study has been conducted on the mutagenic activity of the extract, which has important safety implications. This study evaluated t.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048976
- DOI:
- 10.4238/gmr15048976
Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress
Authors: G.P. Moraes, L.C. Benitez, M.N. do Amaral, I.L. Vighi, P.A. Auler, L.C. da Maia, V.J. Bianchi and E.J.B. Braga
To obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (RT-qPCR) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions. In this study, the stability of expression was evalu.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.24
- DOI:
- 10.4238/2015.March.27.24
LECT2 association with macrophage-mediated killing of Helicobacter pylori by activating NF-?ºB and nitric oxide production
Authors: H.X. Shen, L. Li, Q. Chen, Y.Q. He, C.H. Yu, C.Q. Chu, X.J. Lu and J. Chen
Helicobacter pylori employs unique methods to colonize the stomach, which induces chronic inflammation. It is also able to avoid eradication by macrophages and other immune cells. Leukocyte cell-derived chemotaxin 2 (LECT2), a multi-functional cytokine involved in many pathological conditions, has recently been shown to activa.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048889
- DOI:
- 10.4238/gmr15048889
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
Authors: D?¡rcio ?talo Alves Teixeira, Luciana Magalh?£es Melo, Carlos Alberto de Almeida Gadelha, Rodrigo Maranguape Silva da Cunha, Carlos Bloch Jr., Gandhi R?¡dis-Baptista, Benildo Sousa Cavada and Vicente Jos?© de Figueir?ªdo Freitas
Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In t.. Read More»
- Genet. Mol. Res. 5(1):
Identification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica
Authors: S.T. Ivamoto, D.S. Domingues, L.G.E. Vieira and L.F.P. Pereira
Cytochrome P450s (P450s) comprise a gene superfamily encoding enzymes that are involved in diverse plant metabolic pathways that produce primary and secondary metabolites such as phenylpropanoids, terpenoids, nitrogen-containing compounds, and plant hormones. They comprise one of the most diverse gene families in plant evolution. Although there are many s.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.27.25
- DOI:
- http://dx.doi.org/10.4238/2015.March.27.25
Expression of anti-SRP19 antibody in muscle tissues from patients with autoimmune necrotizing myopathy
Authors: Q. Wang, F. Duan, P. Liu, P.F. Wang and M.X. Wang
This study aimed to investigate the role of anti-SRP19 antibody in muscle tissues of patients with autoimmune necrotizing myopathy. Immunohistochemistry staining was used to determine the expression of anti-SRP19 antibodies in muscle tissues of autoimmune necrotizing myopathy patients. Results demonstrated that anti-SRP19 antibody was expressed in 71.4% (.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038307
- DOI:
- 10.4238/gmr.15038307
Purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from Coffea canephora seeds
Authors: G.C.V. Bard, U. Zottich, T.A.M. Souza, S.F.F. Ribeiro, G.B. Dias, S. Pireda, M. Da Cunha, R. Rodrigues, L.S. Pereira, O.L.T. Machado, A.O. Carvalho and V.M. Gomes
Coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. Antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. We isolated and immunolocalized Cc-LTP2, a new lipid transfer protein (LTP) fr.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048859
- DOI:
- 10.4238/gmr15048859
Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers
Authors: Alexandre Missiaggia and Dario Grattapaglia
We extended the concept of fluorescent microsatellite genotyping with a single-universal tailed primer to the simultaneous use of three different tailed primers to allow multiplexed 4-color detection for medium throughput genotyping of plant species. The method was tested on Eucalyptus DNA samples using three forward primer se.. Read More»
- Genet. Mol. Res. 5(1):
Prognostic role of the cancer stem cell marker CD44 in ovarian cancer: a meta-analysis
Authors: Y.Y. Shi and H. Jiang
This meta-analysis study aimed to investigate the correlation between CD44-positive cancer stem cells (CSCs) and clinicopathological features and its effect on the survival of ovarian cancer patients. A comprehensive literature search in the electronic databases, including PubMed, EMBASE, and Wanfang (up to December 1, 2015), was conducted. Publications a.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038325
- DOI:
- 10.4238/gmr.15038325