Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing
Authors: H. Shen, H.Y. Chen, B. Jia, G.H. Han, Y.S. Zhang and X.C. Zeng
The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understandi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.11
- DOI:
- 10.4238/2015.July.3.11
Development of eight novel microsatellite markers for Huoyan geese
Authors: Z.Z. Cao, D. Su, Y.Y. Zhao, M. Liu, M. Gao and X.H. Luan
In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers we.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.10
- DOI:
- 10.4238/2014.July.25.10
Temporal and tissue expression of genes involved in buds of earliness cotton cultivar
Authors: V.G.L. Batista, M.P.N. Pinheiro, P.A. Melo Filho, R.C. Santos and L.M. Lima
Gene sequences previously identified in Arabidopsis buds were used as references in order to estimate temporal and tissue expression in buds, leaves, stem, and root tissues in cotton plants. Buds were evaluated during 3 phases: 2-8, 10-12, and 14-20 mm. Primers were designed for the ARF6, ATFY, and SEUSS genes for use in semi-quantitative reverse transcri.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.14
- DOI:
- 10.4238/2015.July.3.14
Genetic diversity in intraspecific hybrid populations of Eucommia ulmoides Oliver evaluated from ISSR and SRAP molecular marker analysis
Authors: J. Yu, Y. Wang, M. Ru, L. Peng and Z.S. Liang
Eucommia ulmoides Oliver, the only extant species of Eucommiaceae, is a second-category state-protected endangered plant in China. Evaluation of genetic diversity among some intraspecific hybrid populations of E. ulmoides Oliver is vital for breeding programs and further conservation of this rare species. We studied the genetic diversity of 130 accessions.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.17
- DOI:
- 10.4238/2015.July.3.17
Meiotic behavior and chromosome number of Urochloa adspersa (Trin.) R. D. Webster from the Brazilian Chaco
Authors: M.F. Felismino, R.L.S. Maior, G.A. Damasceno Jr., A. Pott and M.S. Pagliarini
This is the first report of meiotic division in Urochloa adspersa (Trin.) collected from the Brazilian Chaco. Meiotic analyses were performed on three specimens of U. adspersa named G10, G15, and G16. Inflorescences were collected and fixed in a mixture of ethanol and acetic acid (3:1, v/v) for 24 h and then stored in 70% alcohol. Diakinesis revealed diff.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.21
- DOI:
- 10.4238/2015.July.3.21
Boswellic acid attenuates asthma phenotype by downregulation of GATA3 via nhibition of PSTAT6
Authors: X. Zhou, J.G. Cai, W.W. Zhu, H.Y. Zhao, K. Wang and X.F. Zhang
To study the role of boswellic acid in reducing asthma phenotype severity and the relationship between the expression of pSTAT6 and GATA3, thirty-six mice were randomly divided into normal control group, asthma group, and boswellic acid group (treatment group). The asthma model was established through an intraperitoneal injection of sensitization liquid (.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.22
- DOI:
- 10.4238/2015.July.3.22
Effect of melatonin on oncosis of myocardial cells in the myocardial ischemia/reperfusion injury rat and the role of the mitochondrial permeability transition pore
Authors: L.F. Liu, Z.H. Qian, Q. Qin, M. Shi, H. Zhang, X.M. Tao and W.P. Zhu
We aimed to evaluate the effect of melatonin on myocardial cell oncosis in the myocardial ischemia/reperfusion injury rat, and the role of the mitochondrial permeability transition pore (MPTP) therein. Sprague Dawley rats (N = 60) were randomly divided into five groups of 12 rats each: control, ischemia/reperfusion (I/R), melatonin treatment (MT), melaton.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.24
- DOI:
- 10.4238/2015.July.3.24
Proton pump inhibitor use and risk of spontaneous bacterial peritonitis in cirrhotic patients: a systematic review and meta-analysis
Authors: H.B. Xu, H.D. Wang, C.H. Li, S. Ye, M.S. Dong, Q.J. Xia, A.Q. Zhang, K. Pan, X.L. Ge and J.H. Dong
We used a meta-analysis approach to investigate the association between proton pump inhibitor (PPI) use and risk of spontaneous bacterial peritonitis (SBP) in cirrhotic patients. We searched Ovid Medline, Embase, and the Cochrane Library to identify eligible studies. We included studies that compared cirrhotic patients who did or did not use PPIs. The pri.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.25
- DOI:
- 10.4238/2015.July.3.25
Methylenetetrahydrofolate reductase genotypes and haplotypes associated with susceptibility to colorectal cancer in an eastern Chinese Han population
Authors: H. Li, W.L. Xu, H.L. Shen, Q.Y. Chen, L.L. Hui, L.L. Long and X.L. Zhu
Methylenetetrahydrofolate reductase (MTHFR) plays an important role in folate metabolism and is involved in DNA synthesis, DNA repair and DNA methylation. The two common functional polymorphisms of MTHFR, C677T and A1298C have been associated with several diseases, including cancer. We made a case-control study to analyze a po.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.14.8
- DOI:
- 10.4238/2011.December.14.8
Genetic and environmental factors that influence production and quality of milk of Alpine and Saanen goats
Authors: L.F. Brito, F.G. Silva, A.L.P. Melo, G.C. Caetano, R.A. Torres, M.T. Rodrigues and G.R.O. Menezes
Data from 1279 lactations of 783 Alpine and Saanen goats of the herd of our university in Minas Gerais, Brazil, were used to study environmental effects on and to estimate genetic parameters for milk production until 270 days of lactation (MP270) and for production and percentages of fat (PFAT and %FAT), protein (PPROT and %PR.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.14.9
- DOI:
- 10.4238/2011.December.14.9