Molecular cloning and expression analysis of TRAF3 in chicken
Authors: H.L. Yang, Z.Q. Feng, S.Q. Zeng, S.M. Li, Q. Zhu and Y.P. Liu
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.April.30.14
- DOI:
- 10.4238/2015.April.30.14
Assessing genetic variability in bat species of Emballonuridae, Phyllostomidae, Vespertilionidae and Molossidae families (Chiroptera) by RFLP-PCR
Authors: S.R.C. Marchesin1, M.R. Beguelini1, K.C. Faria2, P.R.L. Moreira1 and E. Morielle-Versute1
A PCR-RFLP analysis of the restriction pattern in nuclear (RAG2) and mitochondrial (12S/16S) gene sequences of bat species from the Molossidae, Phyllostomidae, Vespertilionidae, and Emballonuridae families produced a large number of fragments: 107 for RAG2 and 155 for 12S/16S combined in 139 and 402 haplotypes, respectively. The values detected for gene v.. Read More»
- Genet. Mol. Res. 7(4):
- 1164-1178
- DOI:
- 1164-1178
Expression and significance of miR-21 in multiple myeloma patients
Authors: J.H. Wang, W.W. Zhou, B.X. Liu, D.L. Man1, Z.D. Yang, F.R. Liu and H. Shang
The aim of the present study is to examine the expression level of peripheral mir-21 in multiple myeloma (MM) patients and to determine its clinical significance. MM patients (30), monoclonal gammopathy of undetermined significance (MGUS) patients (14), and normal controls (20) were recruited to determine the serum level of β2-MG, IgA and IgM, IgG, &.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016892
- DOI:
- 10.4238/gmr.15016892
Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers
Authors: L.X. Zhou, Y. Xiao, W. Xia and Y.D. Yang
Genetic diversity and patterns of population structure of the 94 oil palm lines were investigated using species-specific simple sequence repeat (SSR) markers. We designed primers for 63 SSR loci based on their flanking sequences and conducted amplification in 94 oil palm DNA samples. The amplification result showed that a rela.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.8.15
- DOI:
- 10.4238/2015.December.8.15
Elevated micronucleus frequencies in patients with pleural plaque secondary to environmental exposure to asbestos
Authors: M. G�¶vercin, A.G. Tomat�±r, F. Evyapan, �°. Acikbas, G. Co��kun and B. Akdag
We examined genotoxic damage and frequency of micronuclei in people living in the Bekilli and Suller districts of Denizli city who had been diagnosed with pleural plaques as a result of environmental exposure to asbestos. Peripheral blood samples were obtained from 30 volunteer patients 59-86 years old who did not smoke or con.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.28.5
- DOI:
- 10.4238/2014.January.28.5
Characterization and expression of DDX6 during gametogenesis in the Chinese mitten crab Eriocheir sinensis
Authors: Q. Li, Y.L. Wang, J. Xie, W.J. Sun, M. Zhu, L. He and Q. Wang
DDX6 belongs to a family of DEAD-box RNA helicases, which are RNA splicing proteins that ensure the correct folding and structure of mature RNA. Gametogenesis requires the participation of many kinds of RNA. To explore its functions during Eriocheir sinensis gametogenesis, we cloned a full-length DDX6 cDNA sequence from E. sinensis (Es-DDX6) which contain.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.April.30.15
- DOI:
- 10.4238/2015.April.30.15
New cytogenetic aberrations found in a case of aggressive retinoblastoma
Authors: J.E.X.S. Barros, E.M. Soares-Ventura, N. Santos, B.A.S. Amaral, F.M. Oliveira, R.S. Vera Cruz, V.L.L. Morais, T.J. Marques-Salles and M.T.C. Muniz
We describe a case of retinoblastoma with an atypical presentation and previously unreported cytogenetic aberrations. A 19-month-old girl with left intraocular retinoblastoma was treated with enucleation and chemotherapy. The disease showed aggressive evolution within a short period between diagnosis and relapse. Eight months .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.15.16
- DOI:
- 10.4238/2012.June.15.16
Comparison of extraction methods of total microbial DNA from freshwater
Authors: P. Li, S.F. Yang, B.B. Lv, K. Zhao, M.F. Lin, S. Zhou, X. Song and X.M. Tang
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugatio.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.16
- DOI:
- 10.4238/2015.January.30.16
CAMK4 gene variation is associated with hypertension in a Uygur population
Authors: S.Z. Chen1,2, Z. Wang1,2, L.N. Zhang1, G.L. Lu2, C.M. Zhou1, D.W. Wang1,Z.M. Tang3, L. Wang2, L. Qin2 and Z.H. Zhai2
Considering that calcium/calmodulin-dependent kinase 4 (CAMK4) plays a pivotal role in blood pressure regulation, we investigated the association between a CAMK4 polymorphism (rs10491334) and hypertension in the Han, Kazak, and Uygur ethnic groups. We studied 1224 patients with hypertension and 967 normotensive controls classified into three ethnic groups.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017207
- DOI:
- 10.4238/gmr.15017207
Non-additive genetic effects on weights and performance of a Brazilian Bos taurus x Bos indicus beef composite
Authors: G.B. Mour�£o, J.B.S. Ferraz2, J.P. Eler2, R.S. Bueno3, J.C.C. Balieiro2, E.C. Mattos2 and L.G.G. Figueiredo4
The aim of the present study was to evaluate the heterosis effects on weaning weight at 205 days (WW, n = 146,464), yearling weight at 390 days (YW, n = 69,315) and weight gain from weaning to yearling (WG, n = 59,307) in composite beef cattle. The fixed models were: RM, which included contemporary groups, class of age of dam, outcrossing percentages for .. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr501
- DOI:
- 10.4238/vol7-4gmr501