Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection
Authors: H.L. Del Puerto, A.S. Martins, G.F. Braz, F. Alves, M.B. Heinemann, D.S. Raj�£o, F.C. Ara�ºjo, S.F. Martins, D.R. Nascimento, R.C. Leite and A.C. Vasconcelos
We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, an.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.18.3
- DOI:
- 10.4238/2011.October.18.3
Efficiency of circulant diallels via mixed models in the selection of papaya genotypes resistant to foliar fungal diseases
Authors: M. Vivas, S.F. Silveira, A.P. Viana, A.T. Amaral Jr., D.L. Cardoso and M.G. Pereira
Diallel crossing methods provide information regarding the performance of genitors between themselves and their hybrid combinations. However, with a large number of parents, the number of hybrid combinations that can be obtained and evaluated become limited. One option regarding the number of parents involved is the adoption o.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.2.9
- DOI:
- 10.4238/2014.July.2.9
Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies
Authors: A.C. Kelly, N.E. Mateus-Pinilla, M. Douglas, M. Douglas, P. Shelton and J. Novakofski
Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for pop.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.19.1
- DOI:
- 10.4238/2011.October.19.1
Optimization of PCR conditions to amplify Cyt b, COI and 12S rRNA gene fragments of Malayan gaur (Bos gaurus hubbacki) mtDNA
Authors: M.K.A. Rosli, A.S. Zamzuriada, S.M.F. Syed-Shabthar, M.C. Mahani, O. Abas-Mazni and B.M. Md-Zain
PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various tri.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.19.2
- DOI:
- 10.4238/2011.October.19.2
Antigenotoxic and antimutagenic effects of glutamine supplementation on mice treated with cisplatin
Authors: J.R. Pesarini, S.G. Victorelli, A.P. Vicentini, L.K. Ferreira, M.O. Mauro, R. Matuo, J.R. Oliveira, A.C.M.B. Antoniolli, M.S. Mantovani and R.J. Oliveira
We evaluated the effects of glutamine on clastogenic and genotoxic damage prevention caused by the administration of cisplatin. Forty Swiss mice were divided into 8 experimental groups: G1 and G2, which were control groups; G3, G4, and G5, which were administered [2 doses of glutamine (orally)] separated by a 24-h period (150,.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.2.11
- DOI:
- 10.4238/2014.July.2.11
Common ABCB1 polymorphisms associated with susceptibility to infantile spasms in the Chinese Han population
Authors: L. Dong, M. Mao, R. Luo, Y. Tong and D. Yu
Infantile spasms are a severe epileptic encephalopathy with a variety of etiologies that occur in infancy and early childhood. Subjects with infantile spasms are at a higher risk for evolving into intractable epileptic spasms, tending to be refractory to conventional antiepileptic drugs. Genetic polymorphisms of the P-glycopro.. Read More»
- Genet. Mol. Res. 10(4):
- 2001.October.19.3
- DOI:
- 10.4238/2001.October.19.3
FAS -670A>G promoter polymorphism is associated with soluble Fas levels in primary Sj�¶grenâ��s syndrome
Authors: B.A. TreviÃ?±o-Talavera, C.A. Palafox-SÃ?¡nchez, J.F. MuÃ?±oz-Valle, G. Orozco-Barocio, R.E. Navarro-HernÃ?¡ndez, M. VÃ?¡zquez-Del Mercado, I. GarcÃ?Âa de la Torre and E. Oregon-Romero
Primary Sjögren’s syndrome (pSS) is a chronic systemic autoimmune disease characterized by lymphocytic infiltration of exocrine glands. Soluble Fas receptor (sFas) has been suggested as a Fas-mediated apoptosis blocker that could impair clonal deletion in infiltrated autoreactive cells. The FAS -670A>G promoter p.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.2.12
- DOI:
- 10.4238/2014.July.2.12
Expression patterns of two heat-shock cognate 70 genes during immune responses and larval development of the Chinese mitten crab Eriocheir sinensis
Authors: P. Li, X.F. Jiang, W.B. Guo, J. Yan and K.Y. Zhou
Two heat-shock protein (HSP) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as EsHSC70-5 and EsHSC70-3, respectively), were isolated from the Chinese mitten crab Eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adul.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15036319
- DOI:
- 10.4238/gmr.15036319
HPV genotyping and p16 expression in Xingu Indigenous Park, Brazil
Authors: V.G. Freitas, G.R. Focchi, E.R. Pereira, J.E. Levi, N.M.G. Speck and J.C. Ribalta
The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15036840
- DOI:
- 10.4238/gmr.15036840
A potential indicator of denervated muscle atrophy: the ratio of myostatin to follistatin in peripheral blood
Authors: R.H. Wu, P. Wang, L. Yang, Y. Li, Y. Liu and M. Liu
Myostatin is a secreted negative regulator of muscle mass, and follistatin antagonizes the function of several members of the TGF-b family, including myostatin. Previously, myostatin expression was found to be closely associated with atrophy of the gastrocnemius muscle, showing a linear correlation, after sciatic nerve injury... Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.21.7
- DOI:
- 10.4238/2011.October.21.7