Optimization of the procedure for extracting nucleic acids from aloe
Authors: J. Li, Y.S. Zhang, C.M. Liaoand X.H. Liu
Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites, which make it difficult to isolate high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of aloe, respectively. The obtained samples presented good quality .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.17.12
- DOI:
- 10.4238/2014.January.17.12
Production and genetic characterization of interspecific hybrids among Crambe abyssinica, Crambe hispanica and Crambe kralikii
Authors: X.Z. Du, B.L. Huang, H. Guan, Z.Y. Li and B.Q. Huang
In this paper, interspecific crosses among Crambe abyssinica, Crambe hispanica, and Crambe kralikii were reported. In the C. hispanica x C. abyssinica (H x A) cross, 118 F1 hybrids were produced without embryo rescue, while 5 F1 hybrids were obtained with embryo rescue, when C. .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.March.26.2
- DOI:
- 10.4238/2014.March.26.2
Mapping quantitative trait loci for the lysozyme level and immunoglobulin G blocking percentage of classical swine fever virus
Authors: X. Lu, Y.F. Gong, J.F. Liu, Y. Liu and Q. Zhang
Increased disease resistance through improved general immune capacity would be beneficial for the welfare and productivity of farm animals. Classical swine fever (CSF) is a contagious disease in farm animals. The immunoglobulin G (IgG) blocking percentage of CSF virus (CSFV) in serum is an essential diagnostic parameter in vet.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.17.13
- DOI:
- 10.4238/2014.January.17.13
RNA-seq reveals the downregulated proteins related to photosynthesis in growth-inhibited rice seedlings induced by low-energy N+ beam implantation
Authors: Q.F. Chen, H.Y. Ya, W.D. Wang and Z. Jiao
Transcriptomic research based on RNA sequencing (RNA-seq) technology is innovative and will provide new opportunities and biological perspectives for the use of ion-beam implantation in plants. Using RNA-seq, transcriptomes of whole rice seedlings generated from seeds implanted with a low-energy N+ beam were analyzed 96 h afte.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.March.26.9
- DOI:
- 10.4238/2014.March.26.9
B chromosome prevalence and physical mapping of 18S rDNA and H4 histone sites in the grasshopper Xyleus discoideus angulatus (Romaleidae)
Authors: C.B. Machado, L.C. Silva Neto, V. Loreto and M.J. Souza
We sampled 11 natural populations of the grasshopper Xyleus discoideus angulatus in Northeastern Brazil to analyze B chromosome frequency and meiotic behavior. We observed a single large B chromosome, resembling the X chromosome, in 29 of the 402 specimens. Eight of the 11 populations had B chromosomes, with a rather .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.March.26.4
- DOI:
- 10.4238/2014.March.26.4
Repair of cartilage defects in BMSCs via CDMP1 gene transfection
Authors: G. Wu, Y. Cui, Y.T. Wang, M. Yao, J. Hu, J.X. Li, Y. Wang andB. Zhang
This study aimed at investigating the ability of cartilage-derived morphogenetic protein 1 (CDMP1) gene-transfected bone marrow mesenchymal stem cells (BMSCs) loaded on the poly(lactic-co-glycolic acid) (PLGA) scaffold for the repair of laryngeal cartilage defects and make a preliminary assessment of its repair effect. The mRN.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.17.14
- DOI:
- 10.4238/2014.January.17.14
Association between serum angiopoietin-2 concentration and clinicopathological parameters in patients with colorectal cancer
Authors: Z.Q. Wang,X.L. Sun,H.L. Su,X.F. Liu,Y.J. Xuan,S.Q. Yu
We examined the expression of angiopoietin-2 in serum samples from patients diagnosed with colorectal cancer and healthy volunteers and investigated the feasibility of using angiopoietin-2 as a potential diagnostic colorectal cancer biomarker. Enzyme-linked immunosorbent assays were used to measure the levels of angiopoietin-2.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.5
- DOI:
- 10.4238/2015.December.1.5
Toll-like receptor 3 polymorphism is not associated with neovascular age-related macular degeneration and polypoidal choroidal vasculopathy in the Chinese
Authors: Y. Cheng, M.W. Li, H.P. Li, W.T. Zeng, P. Zhou, L.Z. Huang, X.X. Li and Y.Y. Sun
Toll-like receptor 3 (TLR3) variants in mainland northern Chinese patients with polypoidal choroidal vasculopathy (PCV) and neovascular age-related macular degeneration (nAMD) were investigated. The complete genes of TLR3, including all exons and the promoter region, were assessed using direct sequencing technology of 284 unre.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.17.15
- DOI:
- 10.4238/2014.January.17.15
Genetic and epigenetic status of triple exotic consanguinity cotton introgression lines
Authors: S.P. He, J.L. Sun and X.M. Du
Introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. The DNA methylation level among 10 introgression lines of cotton (Gossypium hirsutum) and three exotic parental species (G. arboreum, G. thurberi and G. barbadense) were assessed by methylation-s.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.3.1
- DOI:
- 10.4238/2011.October.3.1
Interaction and behavior of virgin and physogastric queens in three Meliponini species (Hymenoptera, Apidae)
Authors: F.H. Nogueira-Ferreira, E.V. Silva-Matos and R. Zucchi
We studied the behavior of virgin queens of the stingless bee species Schwarziana quadripunctata, Paratrigona lineata and Tetragona clavipes, investigating internal nest activities, including the cell provisioning and oviposition process. We made direct observation of queen behavior, with the aid of video filming. Forty-four v.. Read More»
- Genet. Mol. Res. 8(2):