Marker-assisted selection of Fusarium wilt-resistant and gynoecious melon (Cucumis melo L.)
Authors: P. Gao, S. Liu, Q.L. Zhu and F.S. Luan
In this study, molecular markers were designed based on the sex determination genes ACS7 (A) and WIP1 (G) and the domain in the Fusarium oxysporum-resistant gene Fom-2 (F) in order to achieve selection of F. oxysporum-resistant gynoecious melon plants. Markers of A and F are cleaved amplified polymorphic sequences that distinguish alleles according to res.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.8.16
- DOI:
- 10.4238/2015.December.8.16
Screening of mutations in the GCK gene in Jordanian maturity-onset diabetes of the young type 2 (MODY2) patients
Authors: R. Khalil, F. Al-Sheyab, E. Khamaiseh, M.A. Halaweh andH.A. Abder-Rahman
Maturity-onset diabetes of the young type 2 (MODY2) is a genetic form of diabetes mellitus caused by mutations in the glucokinase gene (GCK). We assessed the frequency of GCK gene mutations in Jordanian suspected MODY2 patients. We screened exons 7, 8 and 9, which are specific for pancreatic glucokinase, for mutations at posit.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr597
- DOI:
- 10.4238/vol8-2gmr597
Discovery and characterization of new microsatellite loci in Dipteryx alata Vogel (Fabaceae) using next-generation sequencing data
Authors: R.A. GuimarĂ?£es, M.P.C. Telles, A.M. Antunes, K.M. CorrĂ?ªa,C.V.G. Ribeiro, A.S.G. Coelho and T.N. Soares
The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029639
- DOI:
- 10.4238/gmr16029639
Haplotype distribution of five nuclear genes based on network genealogies and Bayesian inference indicates that Trypanosoma cruzi hybrid strains are polyphyletic
Authors: L. Tomazi, S.Y. Kawashita, P.M. Pereira, B. Zingales andM.R.S. Briones
Chagas disease is still a major public health problem in Latin America. Its causative agent, Trypanosoma cruzi, can be typed into three major groups, T. cruzi I, T. cruzi II and hybrids. These groups each have specific genetic characteristics and epidemiological distributions. Several highly virulent strains are found in the h.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr591
- DOI:
- 10.4238/vol8-2gmr591
Selection of strawberry cultivars with tolerance to Tetranychus urticae (Acari: Tetranychidae) and high yield under different managements
Authors: A.F. Costa, P.E. Teodoro, L.L. Bhering, M.J. Fornazier, J.S. Andrade, D.S. Martins and J.S. Zanuncio Junior
Tetranychus urticae Koch (Acari: Tetranychidae) is considered the main pest of strawberry. Several factors can favor its development, among them the genotype susceptibility and cropping system. The aims of this study were to evaluate the agronomic performance of strawberry cultivars under different managements and to identify .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029599
- DOI:
- 10.4238/gmr16029599
Correlation analysis of serum obestatin expression with insulin resistance in childhood obesity
Authors: M.Y. Zhang, F. Li and J.P. Wang
Childhood obesity is a serious public health concern condition, as excess body fat can negatively affect a child's health. Obestatin is a hormone that regulates body weight by suppressing appetite and reducing food intake; fasting obestatin level is negatively correlated with basal insulin level. This study aims to investigate.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029210
- DOI:
- 10.4238/gmr16029210
A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea)
Authors: Euglena gracilis; Sodium dodecyl sulfate-Tris;RNA isolation; Reverse transcription-polymerase chain reaction
RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit .. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr590
- DOI:
- 10.4238/vol8-2gmr590
Association of polymorphism within the interleukin-28 receptor alpha gene, but not in interleukin-28B, with lower urinary tract symptoms (LUTS) in Chinese
Authors: L. Xiao, L.B. Gao and Q. Wei
The aim of this study was to determine the relationship between polymorphisms in the IL-28B and IL-28R genes and lower urinary tract symptoms (LUTS) in Chinese patients. Genomic DNA was extracted from 553 whole blood samples from 233 patients with LUTS resulted from benign prostatic hyperplasia and 320 control subjects. The IL-28B rs12979860 and rs8099917.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.8
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.8
Phenotypic classification of gastric signet ring cell carcinoma and its relationship with K-ras mutation
Authors: Z.F. Xiong, J. Shi, Z.H. Fu, H.P. Wan and L.X. Tu
We aimed to analyze gastric signet ring cell (SRC) carcinoma subtypes by investigating gastric and intestinal phenotypic marker expression, and explore the relationship between phenotype and K-ras mutation. Immunohistochemistry was performed on 163 SRC carcinoma patient specimens to detect gastric (MUC1, MUC5AC, and MUC6) and .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029181
- DOI:
- 10.4238/gmr16029181
Ă?±-Thalassemia (3.7 kb deletion) in a population from the Brazilian Amazon region: SantarĂ?©m, ParĂ?¡ State
Authors: A.E.S. Souza, G.L. Cardoso, S.Y.L. Takanashi and J.F. Guerreiro
The ethnic composition of the Brazilian population favors high frequencies of the -α3.7 deletion, responsible for α-thalassemia, because this mutation is very common in African populations. In spite of its importance, this hemoglobinopathy has been poorly investigated in Brazil, especially at the molecular level. W.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr601
- DOI:
- 10.4238/vol8-2gmr601