Effects of sample re-sequencing and trimming on the quality and size of assembled consensus sequences
Authors: Prosdocimi F, Lopes DAO, Peixoto FC, Mourao MM, Pacifico LGG, Ribeiro RA and Ortega JM
The production of nucleic acid sequences by automatic DNA sequencer machines is always associated with some base-calling errors. In order to produce a high-quality DNA sequence from a molecule of interest, researchers normally sequence the same besample many times. Considering base-calling errors as rare events, re-sequencing .. Read More»
- Genet. Mol. Res. 6(4):
Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress
Authors: J.K. Gan D.X. Zhang D.L. He X.Q. Zhang Z.Y. Chen Q.B. Luo
DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dr.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.1
- DOI:
- 10.4238/2013.March.15.1
Molecular diversity of Pakistani mango (Mangifera indica L.) varieties based on microsatellite markers
Authors: T. Nazish, G. Shabbir, A. Ali, S. Sami-ul-Allah, M. Naeem, M. Javed,S. Batool, H. Arshad, S.B. Hussain, K. Aslam, R. Seher, M. Tahir and M. Baber
Understanding the genetic diversity of different Pakistani mango varieties is important for germplasm management and varietal characterization. Microsatellites are efficient and highly polymorphic markers for comparative genome mapping, and were used in the present study to determine the genetic relatedness and variability amo.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029560
- DOI:
- 10.4238/gmr16029560
Effect of IL-18 gene promoter polymorphisms on prostate cancer occurrence and prognosis in Han Chinese population
Authors: J.M. Liu J.N. Liu M.T. Wei Y.Z. He Y. Zhou X.B. Song B.W. Ying J. Huang
Interleukin-18 (IL-18) has been implicated in a wide variety of cellular functions that affect the biological response to tumors. However, there is insufficient evidence to prove that IL-18 gene variants are associated with risk of prostate cancer. We examined a possible association between two promoter polymorphisms, -137G/C (rs187238) and -607C/A (rs194651.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.2
- DOI:
- 10.4238/2013.March.15.2
Intermediate alleles of Huntingtonâ??s disease HTT gene in different populations worldwide: a systematic review
Authors: T.A. Apolin?¡rio, C.L.A. Paiva and L.A. Agostinho
Huntington’s disease (HD) is an autosomal dominant progressive neurodegenerative disorder caused by a dynamic mutation due to the expansion of CAG repeats in the HTT gene (4p16.3). The considered normal alleles have less than 27 CAG repeats. Intermediate alleles (IAs) show 27 to 35 CAG repeats and expanded alleles have m.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029648
- DOI:
- 10.4238/gmr16029648
Mitochondrial DNA variability in populations of Centris aenea (Hymenoptera, Apidae), a crop-pollinating bee in Brazil
Authors: V.S. Ferreira C.M.L. Aguiar E.J.F. Oliveira M.A. Costa G.M.M. Santos J.G. Silva
Centris spp are oil-collecting solitary bees that are valuable pollinators of crops such as Brazil nut, cashew, and acerola. We investigated the genetic variability of populations of C. aenea in the northeastern region of Brazil. Total DNA was extracted from 59 individuals from 6 locations in the States of Pernambuco and Bahia and a 600-650-bp fragment of.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.3
- DOI:
- 10.4238/2013.March.15.3
Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR
Authors: H.-J. Xiao, J.-H. Jin, W.-G. Chai and Z.-H. Gong
Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L... Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.10
- DOI:
- 10.4238/2015.January.23.10
Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment
Authors: J.Y. Park Y.S. Yoon H.S. Park S.U. Kuh
We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphat.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.4
- DOI:
- 10.4238/2013.March.15.4
Polymorphism E23K (rs5219) in the KCNJ11 gene in Euro-Brazilian subjects with type 1 and 2 diabetes
Authors: S.W. Souza, L.P. Alcazar, P.A. Arakaki, I.C.R. Santos-Weiss, D. Alberton, G. Picheth and F.G.M. Rego
Insulin secretion is regulated by ATP-sensitive potassium channels (KATP). The potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11) gene, located on chromosome 11p15.1, encodes the subunit Kir6.2 that forms the pore region of KATP channels in pancreatic β-cells. Among the single nucleotide polymorphisms .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029543
- DOI:
- 10.4238/gmr16029543
Optimal clone identifier for genomic shotgun libraries: â??OC Identifier toolâ?
Authors: Cantao ME, Ferreira JE and Lemos EGM
In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of puttin.. Read More»
- Genet. Mol. Res. 6(4):