Three-step method for the amplification of the coxsackievirus A10 genome
Authors: A.Y. Wang1,2*, C.Y. Mu1,3*, C. Chen3, Z. Li1 and Y.H. Xu2
The whole-genome sequencing of coxsackievirus (CV)-A10 does not follow a conventional experimental protocol. To fully understand the genetic variation and evolution of CV-A10, complete genome amplification is necessary. Most previous studies have concentrated on partial sequences of the CV-A10 genome, such as the VP1 gene. The few studies that have invest.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017513
- DOI:
- 10.4238/gmr.15017513
Non-parametric approach to the study of phenotypic stability
Authors: D.F. Ferreira1, S.B. Fernandes2, A.T. Bruzi3 and M.A.P. Ramalho2
The aim of this study was to undertake the theoretical derivations of non-parametric methods, which use linear regressions based on rank order, for stability analyses. These methods were extension different parametric methods used for stability analyses and the result was compared with a standard non-parametric method. Intensive computational methods (e.g.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017517
- DOI:
- 10.4238/gmr.15017517
Salicylic acid-induced changes in physiological parameters and genes of the flavonoid biosynthesis pathway in Artemisia vulgaris and Dendranthema nankingense during aphid feeding
Authors: Y. Sun*, X.L. Xia*, J.F. Jiang, S.M. Chen, F.D. Chen and G.S. Lv
Phloem-feeding aphids cause serious damage to plants. The mechanisms of plant-aphid interactions are only partially understood and involve multiple pathways, including phytohormones. In order to investigate whether salicylic acid (SA) is involved and how it plays a part in the defense response to the aphid Macrosiphoniella sanbourni, physiological changes.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017546
- DOI:
- 10.4238/gmr.15017546
Development of microsatellite markers in the tetraploid fern Ceratopteris thalictroides (Parkeriaceae) using RAD tag sequencing
Authors: X.Y. Yang1*, Z.C. Long2*, A.W. Gichira2, Y.H. Guo1, Q.F. Wang2 and J.M. Chen2
To understand the genetic variability of the tetraploid fern Ceratopteris thalictroides (Parkeriaceae), we described 30 polymorphic microsatellite markers obtained using the restriction site-associated DNA (RAD) tag sequencing technique. A total of 26 individuals were genotyped for each marker. The number of alleles per locus ranged from 4 to 10, and the .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017550
- DOI:
- 10.4238/gmr.15017550
Bovine dopamine receptors DRD1, DRD4, and DRD5: genetic polymorphisms and diversities among ten cattle breeds
Authors: A.M. Sifuentes-Rinc�³n1, A.G. Trejo-Tapia1, R.D. Randel2, P. Ambriz-Morales1 and G.M. Parra-Bracamonte1
The aim of this study was to analyze the allelic frequency distribution and segregation among breeds and/or between different cattle genetic groups of four novel single nucleotide polymorphisms of the bovine DRD1 and DRD5 genes and one reported SNP from the DRD4 gene. One hundred and nine-animals from ten different cattle breeds were genotyped and allelic.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017725
- DOI:
- 10.4238/gmr.15017725
Iron translocation in Pleurotus ostreatus basidiocarps: production, bioavailability, and antioxidant activity
Authors: M.E. Yokota1, P.S. Frison1, R.C. Marcante1, L.F. Jorge1, J.S. Valle1, D.C. Dragunski2, N.B. Colauto1 and G.A. Linde10
Translocation of minerals from substrate to mushrooms can change the medicinal characteristics, commercial value, and biological efficiency of mushroom. In the present study, we demonstrated that addition of iron to the substrate reduces the yield of Pleurotus ostreatus mushroom. The biological efficiency of the mushroom varied from 36.53% on the unsupple.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017888
- DOI:
- 10.4238/gmr.15017888
X-meeting - International Conference of the AB3C
Authors: Roberto M. Cesar-Jr., Marcelo Santoro, Vasco Azevedo
The present special issue of GMR includes selected papers from the X-meeting 2006, held in Fortaleza, CE, Brazil, together with the 14th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2006). Dr. Goran Neshich, EMPRAPA, chaired this seminal event for the Brazilian bioinformatics community, co.. Read More»
- Genet. Mol. Res. 6(4):
Analysis of polymorphisms in codons 11, 72 and 248 of TP53 in Brazilian women with breast cancer
Authors: B.C. Almeida1, J.P.F.O. Kleine1, C.M. Camargo-Kosugi1,2, M.R. Lisboa1,C.N. Fran�§a3, J.P. Fran�§a4 and I.D.C.G. Silva1
The association between TP53 gene polymorphisms and breast cancer (BC) in Brazilian women is a controversial topic. In this cross-sectional study, we evaluated the association between clinical pathological variables and three polymorphisms (TP53*11, TP53*72, and TP53*248) in BC patients and controls. Genomic DNA was extracted from the blood cells of 393 p.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017055
- DOI:
- 10.4238/gmr.15017055
IL10 rs1800896 polymorphism is associated with liver cirrhosis and chronic hepatitis B
Authors: L.N. Cao1, S.L. Cheng2 and W. Liu3
We conducted a case-control study to assess the role of two IL10 gene polymorphisms (rs1800896 and rs1800872) in susceptibility to liver cirrhosis, and their association with chronic hepatitis B in a Chinese population. A case-control study was designed to investigate the association between functional polymorphisms of IL10 (rs1800896 and rs1800872) and t.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017256
- DOI:
- 10.4238/gmr.15017256
Cloning and characterization of defense-related genes from Populus szechuanica infected with rust fungus Melampsora larici-populina
Authors: Z.J. Chen1,2, Z.M. Cao1, Z.D. Yu1 and D. Yu1
Characterization of defense-related genes is critical for breeding disease-resistant poplar varieties and for better management and control of leaf rust disease. In the present study, full-length cDNAs of five Populus szechuanica defense-related (PsDR) genes, pathogen-related protein 1 (PsPR1), β-1,3-glucanase (PsGns), thaumatin-like protein 1 (PsTLP.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017314
- DOI:
- 10.4238/gmr.15017314