Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones
Authors: D. Drakulic, A. Krstic and M. Stevanovic
SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and p.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.9
- DOI:
- 10.4238/2012.May.15.9
A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae)
Authors: J.C. Vazquez-Angulo, V. Mendez-Trujillo, D. GonzÃ?¡lez-Mendoza, A. Morales-Trejo, O. Grimaldo-Juarez and L. Cervantes-DÃ?Âaz
Extraction of high-quality genomic DNA for PCR amplification from filamentous fungi is difficult because of the complex cell wall and the high concentrations of polysaccharides and other secondary metabolites that bind to or co-precipitate with nucleic acids. We developed a modified sodium dodecyl sulfate/phenol protocol, with.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.8
- DOI:
- 10.4238/2012.May.15.8
Association of killer cell immunoglobulin-like receptors with pulmonary tuberculosis in Chinese Han
Authors: C. Lu, Y.-J. Shen, Y.-F. Deng, C.-Y. Wang, G. Fan1, Y.-Q. Liu, S.-M. Zhao, B.-C. Zhang, Y.-R. Zhao, Z.-E. Wang, C.-Z. Zhang and Z.-M. Lu
Killer cell immunoglobulin-like receptors (KIRs) are involved in the pathogenesis of a variety of diseases. However, whether KIR polymorphism is associated with susceptibility to pulmonary tuberculosis was unknown. We examined a possible association of KIR polymorphism with susceptibility to pulmonary tuberculosis in Chinese H.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.7
- DOI:
- 10.4238/2012.May.15.7
Multiclass microarray data classification based on confidence evaluation
Authors: H.L. Yu, S. Gao, B. Qin and J. Zhao
Microarray technology is becoming a powerful tool for clinical diagnosis, as it has potential to discover gene expression patterns that are characteristic for a particular disease. To date, this possibility has received much attention in the context of cancer research, especially in tumor classification. However, most publishe.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.6
- DOI:
- 10.4238/2012.May.15.6
A common genetic variant of 5p15.33 is associated with risk for prostate cancer in the Chinese population
Authors: Q. Ren, B. Xu, S.Q. Chen, Y. Yang, C.Y. Wang, Y.D. Wang, X.H. Wang, L.X. Hua and M. Chen
Recent evidence has suggested that single-nucleotide polymorphisms (SNPs) located at 5p15.33 contribute to susceptibilities for several cancer types, including prostate cancer. To determine whether SNP rs402710 in this region plays a role in prostate cancer, we analyzed these associations in a Chinese population; 251 prostate .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.5
- DOI:
- 10.4238/2012.May.15.5
Identification and characterization of a novel Iraqi isolate of Fusarium pseudograminearum causing crown rot in wheat
Authors: M.A. Hameed, R.M. Rana and Z. Ali
Crown rot is one of the main important fungal diseases affecting wheat in many areas of the world, including Australia, USA, and Iran. Until now, there had been no report of this pathogen in Iraq. Plants displaying crown rot symptoms were observed in Shaat Alarab (Basra, Iraq); we investigated the causal agent of the disease. .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.4
- DOI:
- 10.4238/2012.May.15.4
Expression of Pink1 with �±-synuclein in the dopaminergic neurons of Drosophila leads to increases in both lifespan and healthspan
Authors: A.M. Todd and B.E. Staveley
Overexpression of the gene coding for α-synuclein has been shown to be an inherited cause of Parkinson disease. Our laboratory has previously co-expressed the parkin and Pink1 genes to rescue α-synuclein-induced phenotypes within a Drosophila model. To further investigate the effect of Pink1 in this model, we perfo.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.6
- DOI:
- 10.4238/2012.May.21.6
Association of GSTM1 and GSTT1 with ageing in auto repair shop workers
Authors: S.A. Eshkoor, S.J. Marashi, P. Ismail, S.A. Rahman, M. Mirinargesi, M.Y. Adon and R.V. Devan
We evaluated the possible influence of glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta (GSTT1) genes on genetic damage due to occupational exposure, which contributes to accelerate ageing. This study was conducted on 120 car auto repair workshop workers exposed to occupational hazards and 120 controls .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.5
- DOI:
- 10.4238/2012.May.21.5
Molecular characterization of a mariner-like element in the Atta sexdens rubropilosa genome
Authors: P. Rezende-Teixeira, J.B. do Amaral, F. Siviero and G.M. Machado-Santelli
Mobile elements are widely present in eukaryotic genomes. They are repeated DNA segments that are able to move from one locus to another within the genome. They are divided into two main categories, depending on their mechanism of transposition, involving RNA (class I) or DNA (class II) molecules. The mariner-like elements are.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.4
- DOI:
- 10.4238/2012.May.21.4
Development of microsatellite markers for the small yellow croaker Larimichthys polyactis (Sciaenidae) by cross-species amplification
Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang
The small yellow croaker (Larimichthys polyactis) is a highly valued fish for human consumption found in the Western Pacific that was considered endangered until recently because of overfishing. We selected microsatellite markers for this species from markers developed for Miichthys miiuy, also of the family Sciaenidae. Among .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.3
- DOI:
- 10.4238/2012.May.21.3