Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers
Authors: L. Zhang H.G. Zhang X.F. Li
Dahurian larch (Larix gmelinii), a deciduous conifer, is the northernmost tree, native to eastern Siberia and nearby regions of China. We used growth traits and molecular markers to assess genetic variation in different L. gmelinii growing regions; 105 individual samples were collected from seven regions of the Qingshan Forestry Centre, Heilongjiang Province.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.12
- DOI:
- 10.4238/2013.January.24.12
Isolation and characterization of white and brown adipocytes in Kunming mice
Authors: Y.W. Nie*, P. Zhang*, J. Zhang, H.Y. Liang, M. Wang, B. Dai, H. Liangand D.J. Liu
White adipose tissue and brown adipose tissue play critical roles in controlling energy homeostasis and the development of obesity and diabetes. We isolated mouse white adipocytes from inguinal white fat tissues and brown adipocytes from interscapular brown fat tissues, and employed a variety of approaches, including immunofluorescent staining, quantitati.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017355
- DOI:
- 10.4238/gmr.15017355
Abnormal metabolites related to bone marrow failure in aplastic anemia patients
Authors: P. Zhong, J. Zhang and X. Cui
Metabolomics is the identification and quantitation of small molecules that are involved in metabolic reactions. Liquid chromatography-mass spectrometry (LC-MS) has enjoyed growing popularity as the platform for metabolomic studies due to high throughput, soft ionization, and good coverage of metabolites. Serum metabolites of aplastic anemia (AA) patients.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.33
- DOI:
- 10.4238/2015.October.28.33
Karyotype studies on Lycoris radiata populations from China
Authors: Y.X. Liu1, Y.H. Zheng1,2, T. Xia1 and J. Zhou1
Lycoris radiata is an important medicinal and ornamental plant of China. In the present study, somatic chromosome counts and karyotype analyses, which are important aspects of plant phylogeny and evolution, were performed in 466 individuals from 25 L. radiata populations by root tip squash method. Chromosome counts revealed that 10 populations were diploi.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017357
- DOI:
- 10.4238/gmr.15017357
Effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus
Authors: Q.D. Jiang1,2 and X.P. Li1
The hypothalamus is an important component of the nervous system, and neuropeptide Y (NPY), proopiomelanocortin (POMC), and neuromedin U (NMU) are key players in physiological regulation. Puerarin is important for nerve regulation. We investigated the effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus. The results showed .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017362
- DOI:
- 10.4238/gmr.15017362
Efficient isolation of high-quality RNA from lotus Nelumbo nucifera ssp nucifera tissues
Authors: Y.J. Zhang X.Y. Hao Z.S. Liang W.D. Ke H.B. Guo
Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-qua.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.14
- DOI:
- 10.4238/2013.January.24.14
Structure characterization of human cytomegalovirus UL131A, UL130 and UL128 genes in clinical strains in China
Authors: Z.-R. Sun, Y.-H. Ji, Q. Ruan, R. He, Y.-P. Ma, Y. Qi, Z.-Q. Mao,Y.-J. Huang
Human cytomegalovirus (HCMV) genetic determinants of endothelial cell tropism, leukocytes and dendritic cells have been identified in the genes UL131A, UL130, and UL128. We examined the structure of these three genes in HCMV. Eighteen low-passage clinical isolates and five non-passage strains from congenitally HCMV-infected in.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr654
- DOI:
- 10.4238/vol8-3gmr654
Epistasis in intra- and inter-gene pool crosses of the common bean
Authors: J.C. Borel1, M.A.P. Ramalho2 and A.F.B. Abreu3
Epistasis has been shown to have an important role in the genetic control of several quantitative traits in the common bean. This study aimed to investigate the occurrence of epistasis in intra- and inter-pool gene crosses of the common bean. Four elite lines adapted to Brazilian conditions were used as parents, two from the Andean gene pool (ESAL 686; BR.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017573
- DOI:
- 10.4238/gmr.15017573
Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing
Authors: L.A. Fusinatto S. Lopes A. Silva-Ferreira J. Alexandrino C.F.B. Haddad C.F.D. Rocha F. Sequeira
The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.15
- DOI:
- 10.4238/2013.January.24.15
Genetic variability and heterogeneity of Venezuelan equine encephalitis virus vector Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations of the Colombian Atlantic coast, based on microsatellite loci
Authors: F. Bello and V. Becerra
In Colombia, the mosquito Ochlerotatus taeniorhynchus has been identified as an efficient vector of the epidemic-epizootic Venezuelan equine encephalitis virus. We evaluated the genetic variability and heterogeneity of this mosquito in Colombian populations using eight microsatellite DNA loci. Two hundred and te.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr652
- DOI:
- 10.4238/vol8-3gmr652