Selecting black-spot resistant papaya genotypes derived from backcrossing and hybrids
Authors: T.P.S. Poltronieri, S.F. Silveira, M. Vivas, R. Santa Catarina,D.F.M. Cortes, A.O.N. Azevedo and M.G. Pereira
Papaya crop is important to Brazilian agribusiness. However, the expansion of papaya cultivation in the country is affected by the absence of commercial cultivars presenting good disease resistance. The black-spot caused by the fungus Asperisporium caricae is the most damaging foliar disease affecting Brazilian papaya crops. T.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019401
- DOI:
- 10.4238/gmr16019401
Frequency of the S65C mutation in the hemochromatosis gene in Brazil
Authors: V.C. Oliveira1,2, F.A. Caxito1, K.B. Gomes3 , A.M. Castro1,V.C. Pardini1 and A.C.S. Ferreira1,2
Development of hereditary hemochromatosis is associated with the C282Y, H63D or S65C mutations in the hemochromatosis gene. Though there is extensive knowledge about the former two, there is little information on the mechanism of action and the allelic frequency of the S65C mutation. We examined the prevalence of the S65C mutation of the hemochr.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr562
- DOI:
- 10.4238/vol8-3gmr562
Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)
Authors: C.Y. Pang T.X. Deng D.S. Tang C.Y. Yang H. Jiang B.Z. Yang X.W. Liang
The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, inclu.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.3
- DOI:
- 10.4238/2012.July.10.3
Prediction of phosphorylation sites based on the integration of multiple classifiers
Authors: R.Z. Han, D. Wang, Y.H. Chen, L.K. Dong and Y.L. Fan
Phosphorylation is an important part of post-translational modifications of proteins, and is essential for many biological activities. Phosphorylation and dephosphorylation can regulate signal transduction, gene expression, and cell cycle regulation in many cellular processes. Phosphorylation is extremely important for both ba.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019354
- DOI:
- 10.4238/gmr16019354
Molecular cloning, sequencing, and expression of Eimeria tenella HSP70 partial gene
Authors: A.L.G. Bogado, G.F. Martins, J.P. Sasse, J. da S. Guimar�£es Jr., J.L. Garcia
Members of the Eimeria genus are protozoan parasites of the subphylum Apicomplexa (Eimeriidae family), and belong to the coccidia group. Eimeria tenella is one of the most pathogenic species owing to its ability to penetrate the mucosa, and cause inflammation and damage. It is an obligate intracellular parasite that causes dis.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16018964
- DOI:
- 10.4238/gmr16018964
Variation in genotypic responses and biochemical analysis of callus induction in cultivated wheat
Authors: M.M. Shah1, Q. Khalid1, U.W. Khan1, S.A.H. Shah3, S.H. Shah2,A. Hassan1 and A. Pervez1
Wheat is notorious for callus induction, which is a major hindrance in direct gene transfer and consequently for genetic improvement programs. In order to provide a successful platform for gene transformation, good callus quantity and quality is important. We investigated the variation in callus induction capabilities of Pakistani wheat cultivars and.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr513
- DOI:
- 10.4238/vol8-3gmr513
Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)
Authors: X.J. Chen Z.B. Li L. Chen Y.Y. Cao Q.H. Li
The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.4
- DOI:
- 10.4238/2012.May.22.4
Expression of metallothionein type 2 and 3 genes in Prosopis glandulosa leaves treated with copper
Authors: C. Michel-Lopez, O. Zapata-P�©rez, D. Gonz�¡lez-Mendoza,O. Grimaldo-Juarez, C. Cece�±a-Duran, O. Tzintzun-Camacho
For a better understanding of the strategies that are used by Prosopis glandulosa in heavy metal tolerance, the present study evaluated the gene expression of three metallothioneins (MTs; PgMt2-1, PgMt2, and PgMt3) in plants exposed to sub-lethal concentrations of copper. The PgMt2-1, PgMt2, and PgMt3 sequences were homologous.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019490
- DOI:
- 10.4238/gmr16019490
The radiotracer 99mTc-MIBI is not genotoxic for human peripheral blood lymphocytes at diagnostic radioactive dose
Authors: S.J. Hosseinimehr1, A. Ahmadi1, D. Beiki2, A. Mahmoudzadeh3, M. Babaei4 and E. Habibi1
The radiotracer technetium-99m methoxyisobutyl isonitrile (99mTc-MIBI) has been widely used for myocardial blood flow imaging. We investigated the genotoxicity of 99mTc-MIBI in cultured human lymphocytes at the same concentration used in patients. Radioactivity doses were determined in whole blood at 5 min post-injection of 20 mCi 99mTc-MIBI in patients. .. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr602
- DOI:
- 10.4238/vol8-3gmr602
Insight into gene evolution within Cervidae and Bovidae through genetic variation in MHC-DQA in the black muntjac (Muntiacus crinifrons)
Authors: H.-L. Wu C.-C. Tong E. Li T.-L. Luo
The critical role that the major histocompatibility complex plays in the immune recognition of parasites and pathogens makes its evolutionary dynamics exceptionally relevant to ecology, population biology, and conservation studies. The black muntjac is a rare deer endemic to a small mountainous region in eastern China. We found that this species has two D.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.15.13
- DOI:
- 10.4238/2012.May.15.13