Abstract

Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)

Author(s): X.J. Chen Z.B. Li L. Chen Y.Y. Cao Q.H. Li

The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of polymorphic alleles per locus ranged from 2 to 11. The observed and expected heterozygosities were 0.050-0.913 and 0.049-0.869, respectively. The loci identified in this study could provide a useful tool for research on genetic diversity and genetic differentiation of A. pectinata populations. The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of polymorphic alleles per locus ranged from 2 to 11. The observed and expected heterozygosities were 0.050-0.913 and 0.049-0.869, respectively. The loci identified in this study could provide a useful tool for research on genetic diversity and genetic differentiation of A. pectinata populations.

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