Combination of multiple gene markers to detect circulating tumor cells in the peripheral blood of patients with non-small cell lung cancer using real-time PCR
Authors: M. Ding, X. Li and T. Qiu
Our study aims to determine the clinical significance of human telomerase reverse transcriptase (hTERT), S-phase kinase-associated protein 2 (Skp2) and thyroid transcription factor-1 (TTF-1) mRNA expressions in peripheral blood (PB) of patients with non-small cell lung cancer (NSCLC). Real-time polymerase chain reaction was used to investigate the gene ex.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.24
- DOI:
- 10.4238/2015.October.21.24
Circulating B7-H4 in serum predicts prognosis in patients with hepatocellular carcinoma
Authors: S.A. Zhang, Z.X. Wu, X. Zhang, Z.Y. Zeng and D.L. Li
B7-H4 is member of the B7 family that negatively regulates the immune response, which are important for fine-tuning of the tumor microenvironment. Dysregulation of B7-H4 expression has been associated with tumor progression. However, expression level of B7-H4 in hepatocellular carcinoma (HCC) tissues is still a controversial topic. In addition, whether se.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.25
- DOI:
- 10.4238/2015.October.21.25
Association of CRTC2 gene polymorphisms with growth and meat quality traits of Qinchuan cattle
Authors: H.C. Xu, L.S. Gui, N. Song, Y.Y. Zhang, H.C.Wang and L.S. Zan
Growth and meat quality traits play important roles in the evaluation of cattle productivity and are influenced by genetic and environmental factors. CRTC2 is a recently discovered gene related to obesity that may influence fat deposition. The aim of the current study was to detect polymorphisms of bovine CRTC2 and explore their relationships to growth an.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.12
- DOI:
- 10.4238/2015.October.21.12
Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis.
Authors: Y.W. Huang, Y.J. Tsai and F.C. Chen
Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology obs.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.11
- DOI:
- 10.4238/2014.December.18.11
Genome-wide identification of copy number variations in Holstein cattle from Baja California, Mexico, using high-density SNP genotyping arrays
Authors: R. Salom�³n-Torres, V.M. Gonz�¡lez-Vizcarra, G.E. Medina-Basulto,M.F. Monta�±o-G�³mez, P. Mahadevan, V.H. Yaurima-Basald�ºa, C. Villa-Angulo1 and R.Villa-Angulo
Copy number variations (CNVs) are an important source of genomic structural variation, and can be used as markers to investigate phenotypic and economic traits. CNVs also have functional effects on gene expression and can contribute to disease susceptibility in mammals. Currently, single nucleotide polymorphism genotyping arra.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.2.18
- DOI:
- 10.4238/2015.October.2.18
Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes
Authors: Y.H. Zhao, Z.B. Zhang, C.Q. Zhao, Y. Zhang, Y.F. Wang, W.J. Guan and Z.Q. Zhu
The genes of top athletes are a valuable genetic resource for the human race, and could be exploited to identify novel genes related to sports ability, as well as other functions. We analyzed the expressed sequence tags from top half-pipe snowboarding athletes using the SMART complementary DNA (cDNA) library construction method to elucidate the characteri.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.13
- DOI:
- 10.4238/2015.October.21.13
Regulation of the expression of zinc finger protein genes by microRNAs enriched within acute lymphoblastic leukemia-derived microvesicles
Authors: L. Lu, X.M. Chen, H.M. Tao, W. Xiong, S.H. Jie and H.Y. Li
Microvesicles (MVs) are submicrometric membrane fragments that can “engulf” cytoplasmic contents such as microRNAs (miRNAs) from their cellular origin. The study of miRNAs carried within MVs might provide insights into the roles that miRNAs play in the underlying pathophysiologic processes of acute lymphoblastic le.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.2
- DOI:
- 10.4238/2015.October.5.2
Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)
Authors: J.M. Lai, C.C. Tsai, C.R. Yen, Y.Z. Ko, S.R. Chen, I.S. Weng, Y.S. Lin and Y.C. Chiang
Syzygium samarangense (Blume) Merr. & Perry (wax apple) is an important commercial fruit tree in Southeast Asia. Here, microsatellite markers were developed to evaluate genetic diversity and distinguish cultivars in this species. In total, 161 microsatellite loci with sufficient flanking sequences to design primer sets were isolated from wax apple usi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.22
- DOI:
- 10.4238/2015.October.21.22
Inhibition of gap junctions relieves the hepatotoxicity of TNF-�±
Authors: Z.Y. Chen, R. Wang, F. Huang, D.D. Yuan and S.R. Li
The aim of this study was to observe the influence of gap junction (GJ) functional changes on the hepatotoxicity of TNF-α. Three different methods were employed to study functional effects of the GJ inhibition: 1) pretreatment with a GJ inhibitor; 2) inoculation of cells at high and low densities; and 3) inhibition of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.3
- DOI:
- 10.4238/2015.October.5.3
Influence of various quantitative trait loci (QTL) mapping methods on the mapping accuracy under varying heritability levels
Authors: C.F. Su, Z.B. Liu and Y.G. Li
The study of quantitative trait effects is of great significance for molecular marker-assisted breeding. The accuracy of quantitative trait loci (QTL) mapping is the key factor affecting marker-assisted breeding, and is extremely significant. The effect of different heritability rates (10, 30, 50, 70, and 90%) on the accuracy of QTL mapping of five recomb.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.21
- DOI:
- 10.4238/2015.October.21.21