Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation
Authors: J. Du, Z.H. Tao, J. Li , Y.K. Liu and L. Gan
We constructed hepatocellular carcinoma (HCC) cells that stably express stathmin with a Ser25 phosphorylation site mutation (stathmin S25A). We used the polymerase chain reaction for site-directed mutagenesis, constructed a stathmin S25A plasmid, and verified the results by restriction enzyme cleavage and sequencing technology.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.24
- DOI:
- 10.4238/2015.October.5.24
Breeding of a target genotype variety based on identified chalkiness marker-QTL associations in rice (Oryza sativa L.)
Authors: X. Liu, Y.R. Du, X.H. Li, X.L. Li, W.Q. Yang and Y. Wang
The aim of this study was to breed a target genotype variety based on the identified chalkiness marker-QTL (quantitative trait locus) associations in rice. First, a permanent mapping population of rice that consisted of 525 recombinant inbred lines (RILs), which were derived from Zhenshan 97/Minghui 63, was used to identify QTLs with additive effects for .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.10
- DOI:
- 10.4238/2015.October.21.10
Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer
Authors: A.R. Ferreira, L.F.C. Aguiar Filho, R.V. Sousa, R. Sartori and M.M. Franco
Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Tota.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.26
- DOI:
- 10.4238/2015.October.5.26
Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects
Authors: R.P. Araldi, T.C. Melo, A.C. Neves, D.D. Spadacci-Morena, R.F. Magnelli, D.G. Modolo, P.L. de-S�¡-J�ºnior, J. Mazucchelli-de-Souza, R.F. Carvalho, W. Be�§ak and R.C. Stocco
The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.15
- DOI:
- 10.4238/2015.October.21.15
miR-71b regulation of insulin/IGF-1 signaling during starvation in planarians
Authors: Y.Y. Wu, J.M. Zhao, Q. Liu, Q. Guo, Z. Liu, X.X. Wang, C.Y. Wang, R.Y. Li, Y.Z. Zhang and S.T. Zhang
Planarians, which have a large population of stem cells called neoblasts, are molecularly tractable model systems used in the study of regeneration. However, planarians have strong resistance to hunger and have developed growth arrest strategies. For example, they can change their size and undergo growth regression during star.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.4
- DOI:
- 10.4238/2015.October.5.4
Changes in cFLIP expression in the corpus luteum throughout the estrous cycle of Shibagoats
Authors: H.Z. Jin, Y. Wang, Y. Liu, X.P. Li and Y. Jin
Changes in the expression of the anti-apoptotic protein cFLIP (cellular FLICE inhibitory protein) were examined in the caprine corpus luteum (CL), during development and subsequent maintenance. Corpora lutea at four different stages were collected from Shiba goats, to measure the expression of cFLIP mRNA, protein and immunolocalization. Expression of shor.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.16
- DOI:
- 10.4238/2015.October.21.16
Repairing rabbit radial defects by combining bone marrow stroma stem cells with bone scaffold material comprising a core-cladding structure
Authors: H. Wu, G.H. Liu, Q. Wu and B. Yu
We prepared a bone scaffold material comprising a PLGA/β-TCP core and a Type I collagen cladding, and recombined it with bone marrow stroma stem cells (BMSCs) to evaluate its potential for use in bone tissue engineering by in vivo and in vitro experiments. PLGA/β-TCP without a cladding was used for comparison. The ad.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.7
- DOI:
- 10.4238/2015.October.5.7
Clinical characteristics of patients with non-small cell lung cancers harboring anaplastic lymphoma kinase rearrangements and primary lung adenocarcinoma harboring epidermal growth factor receptor mutations
Authors: L. Xu, J. Lei, Q.Z. Wang, J. Li and L. Wu
Echinoderm microtubule associated protein like 4-anaplastic lymphoma kinase (EML4-ALK) gene rearrangements and epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) have been intensively studied. The objective of this study was to determine the clinicopathological characteristics in genotype-specific subsets of patients w.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.18
- DOI:
- 10.4238/2015.October.21.18
Association of A-20C polymorphism in the angiotensinogen gene with essential hypertension: a meta-analysis
Authors: R. Zeng, Q.P. Wang, M.X. Fang, J. Zhuang and R.X. Fan
The A-20C polymorphism in the angiotensinogen (AGT) gene has been associated with increased risk of essential hypertension in several studies; however, these studies gave inconsistent results. In this study, we performed a meta-analysis to assess the association between AGT A-20C polymorphism and essential hypertension. Published literature was retrieved .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.19
- DOI:
- 10.4238/2015.October.21.19
LITAF, DUSP1 and HHEX expression in mesenchymal stem cells from patients with psoriasis.
Authors: W.J. Chang, X.P. Niu, R.X. Hou, J.Q. Li, R.F. Liu, Q. Wang1, C.F. Wang,X.H. Li, G.H. Yin and K.M. Zhang
Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation sta.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.31
- DOI:
- 10.4238/2015.December.1.31