Distribution and localization of abnormally expressed OPTN proteins in RGC5 retinal ganglion cells and their effects on subcellular morphology
Authors: Z.R. Xu, F.G. Jiang and F. Chen
The objectives of this study were to investigate the distributions of abnormally expressed optineurin (OPTN) proteins in retinal ganglion cells (RGC5s) of transgenic rats and their effects on subcellular morphological structures. Green fluorescent protein labeled EGFP wild-type (OPTNWT), E50K mutant type (OPTNE50K), and OPTN s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.22
- DOI:
- 10.4238/2015.October.5.22
An improved strategy based on RAPD markers efficiently identified 95 peach cultivars
Authors: M.L. Yu, W.Y. Wang, R.J. Ma, Z.J. Shen and J.G. Fang
DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.7.1
- DOI:
- 10.4238/2012.May.7.1
Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9
Authors: X. Sun1, N.J. Xu1, L.Z. Jiang1, W. Hu1, M.H. Fan1 and Z.M. Liu2
A strain of the microalga Chlorella pyrenoidosa F-9 in our laboratory showed special characteristics when transferred from autotrophic to heterotrophic culture. In order to elucidate the possible metabolic mechanism, the gene expression profiles of the autonomous organelles in the green alga C. pyrenoidosa under autotrophic and heterotrophic cultivation w.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.17
- DOI:
- 10.4238/2014.October.20.17
Molecular cloning and expression analysis of five GhRAXs in upland cotton (Gossypium hirsutum L.)
Authors: T.C. Dai and Z.M. Wang
The formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. Several transcription factor-encoding genes, including CUC3, RAX, LAS, LOF1, and ROX, have been cloned by screening for axillary meristem mutants in Arabidopsis thaliana. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.25
- DOI:
- 10.4238/2015.October.5.25
Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR
Authors: C. Wang, C.-L. Zhao, L. Liu, R. Wu and X.-L. Zhang
Doppel is a homologue of cellular prion protein (PrP)-like protein (PrPC). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA wa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.3
- DOI:
- 10.4238/2015.October.9.3
Cytoplasmic and extracellular expression of pharmaceutical-grade mycobacterial 65-kDa heat shock protein in Lactococcus lactis
Authors: M.S.P. de Azevedo, C.S. Rocha, N. Electo, D.S. Pontes, J.B. Molfetta, E.D.C. Gon�§alves, V. Azevedo, C.L. Silva and A. Miyoshi
Lactic acid bacteria (LAB) are an attractive and safe alternative for the expression of heterologous proteins, as they are nonpathogenic and endotoxin-free organisms. Lactococcus lactis, the LAB model organism, has been extensively employed in the biotechnology field for large-scale production of heterologous proteins, and its.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.14
- DOI:
- 10.4238/2012.April.27.14
Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance
Authors: M. Lu*, L. Wang*, J. Zhang, S. Sun, Y. Li, W. Du, J. Wu, J. Zhao, Q. Yang and X. Chen
A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.10
- DOI:
- 10.4238/2014.December.18.10
Association between MYC rs9642880[T] allele and bladder cancer risk: a meta-analysis
Authors: Y. Zhao, J.G. Qi, N. Yang, Y.L. Lin, J. Liang and X. Zhu
A single nucleotide polymorphism of MYC rs9642880 (G>T) at the 8q24.1 locus is thought to be associated with bladder cancer risk based on the results of genome-wide association studies, but the results remain inconclusive. To assess the association between rs9642880[T] allele and bladder cancer risk, we performed this meta-analysis including 18 case-co.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.39
- DOI:
- 10.4238/2015.November.18.39
Association analysis of IL-17A and IL-17F polymorphisms in Chinese women with cervical cancer
Authors: L. Li, Y.L. Tian, X.M. Lv, H.F. Yu, Y.Y. Xie, J.D. Wang and W. Shi
We selected six tagged single nucleotide polymorphisms (SNPs) in the interleukin 17A (IL-17A) and IL-17F genes, and evaluated the relationship between the six common SNPs and environmental factors in cervical cancer patients. Polymerase chain reaction-restriction fragment length polymorphism was used to detect the IL-17A (rs22.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.6
- DOI:
- 10.4238/2015.October.9.6
Assessment of correlation between pre-miRNA-1757 polymorphism and chicken performance traits
Authors: H. Li, K.R. Jiang, S.H. Wang, X.J. Liu, X.T. Kang, R.R. Jiang,Z.J. Li and G.R. Sun
Single nucleotide polymorphism in microRNAs (miRNA) may influence their target gene selection and regulation efficiency, leading to animal phenotypic variation. The aim of this study was to evaluate the possible effect of single nucleotide polymorphisms in the miRNA-1757 gene precursor region (pre-mir-1757) on economic-related.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.7
- DOI:
- 10.4238/2015.October.9.7