Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody
Authors: W.Y. Zheng, Y. Wang, Z.C. Zhang and F. Yan
We examined the immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody. Genomic DNA from the M5 strain of goat Brucella was amplified by polymerase chain reaction and cloned into the prokaryotic expression vector pGEX-4T-1. The expression and immunological characteristics of .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.10
- DOI:
- 10.4238/2015.October.5.10
Cloning flanking sequence by single-primer PCR in transgenic plants
Authors: J. Ma1, Y.P. Wang1, S. Ren2, Z. Zhang1, S. Lu1 and P.W. Wang1
The insertion position of exogenous genes in plant genomes is usually identified by adapter ligation-mediated polymerase chain reaction (PCR), thermal asymmetric interlaced PCR, and restriction site extension PCR in transgenic plant research. However, these methods have various limitations, such as the complexity of designing primers and time-consuming an.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.16
- DOI:
- 10.4238/2014.October.20.16
AMP-activated protein kinase regulates autophagic protection against cisplatin-induced tissue injury in the kidney
Authors: L. Wei, W. Chen, Y. Zou, H. Huang, B. Pan, S. Jin, R. Huang, S. Nie and G. Kong
Although the nephrotoxicity of cisplatin has been well documented as a major side effect of chemotherapy, the exact mechanism by which prosurvival and apoptotic pathways interplay to determine renal pathology remains elusive. Recent studies suggested that autophagy might serve as an adaptive mechanism to promote cell survival .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.13
- DOI:
- 10.4238/2015.October.5.13
Investigations on the genomic diversity of OXA from isolated Acinetobacter baumannii
Authors: Z. Ma, L.Q. Zhou, H. Wang and L.P. Luo
We distinguished the four OXA-type carbapenemase subgroup alleles present in 120 strains of Acinetobacter baumannii by using polymerase chain reaction (PCR) and investigated the distributions of the OXA subgroups in clinically isolated samples. Amplification of the OXA genes blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 was performed by multiplex PCR. An.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.36
- DOI:
- 10.4238/2015.November.18.36
Multivariate analysis in a genetic divergence study of Psidium guajava
Authors: A.M. Nogueira, M.F.S. Ferreira, J.H.S. Guilhen and A. Ferreira
The family Myrtaceae is widespread in the Atlantic Forest and is well-represented in the Espírito Santo State in Brazil. In the genus Psidium of this family, guava (Psidium guajava L.) is the most economically important species. Guava is widely cultivated in tropical and subtropical countries; however, the widespread cultivation of only a small num.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.8
- DOI:
- 10.4238/2014.December.18.8
Molecular cloning and characterization of a proliferating cell nuclear antigen gene by chemically induced male sterility in wheat (Triticum aestivum L.)
Authors: Y.A. Yu, G.S. Zhang, J. Zhang, L. Ju, Q.D. Zhu, Y.L. Song, J.W. Wang, N. Niu and S.C. Ma
Although a number of studies have shown that chemical hybridizing agents (CHAs) affect anther growth and regulate cell-cycle progression, little is known about the molecular and cellular mechanisms involved. Proliferating cell nuclear antigen (PCNA) is an essential factor in DNA replication, and in many other processes in euka.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.16
- DOI:
- 10.4238/2015.October.5.16
R620W functional polymorphism of protein tyrosine phosphatase non-receptor type 22 is not associated with pulmonary tuberculosis in Zahedan, southeast Iran
Authors: H.-R. Kouhpayeh, M. Hashemi, S.-A. Hashemi, A. Moazeni-Roodi, M. Naderi, B. Sharifi-Mood, M. Taheri, M. Mohammadi and S. Ghavami
The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene, which encodes an intracellular lymphoid-specific phosphatase, is considered an important regulator of T-cell activation. We investigated a possible association between the PTPN22 C1858T (R620W) polymorphism and pulmonary tuberculosis in an Iranian population... Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.6
- DOI:
- 10.4238/2012.April.27.6
Leishmania (Viannia) braziliensis in dogs in Brazil: epidemiology, co-infection, and clinical aspects
Authors: F.S. Carvalho, A.A. Wenceslau, G.R. Albuquerque, A.D. Munhoz, E. Gross, P.L.S. Carneiro, H.C. Oliveira, J.M. Rocha, I.A. Santos and R.P. Rezende
Leishmaniasis is an endemic disease present in 98 countries. In Brazil, the northeast region accounts for approximately half of the cases in humans, and has experienced an increased number of positive cases in dogs. In this study, we investigated the epidemiology of canine leishmaniasis in the city of Ilhéus, Bahia, usi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.19
- DOI:
- 10.4238/2015.October.5.19
Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15
Authors: Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15
Glyphosate and glyphosate-containing herbicides have an adverse effect on mammals, humans, and soil microbial ecosystems. Therefore, it is important to develop methods for enhancing glyphosate degradation in soil through bioremediation. We investigated the potential of glyphosate degradation and bioremediation in soil by Bacillus subtilis Bs-15. Bs-15 gre.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.37
- DOI:
- 10.4238/2015.November.18.37
Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii
Authors: N.Z. Zhang, S.Y. Huang, D.H. Zhou, Y. Xu, J.J. He and X.Q. Zhu
Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.9
- DOI:
- 10.4238/2014.December.18.9