Detection of Y chromosome microdeletions and mitochondrial DNA mutations in male infertility patients
Authors: A.I. G�¼ney, D. Javadova, D. K�±rac, K. Ulucan, G. Koc, D. Ergec, H. Tavukcu and T. Tarcan
Infertility affects about 10-15% of all couples attempting pregnancy with infertility attributed to the male partner in approximately half of the cases. Proposed causes of male infertility include sperm motility disturbances, Y chromosome microdeletions, chromosomal abnormalities, single gene mutations, and sperm mitochondrial.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.2
- DOI:
- 10.4238/2012.April.27.2
Interaction of six candidate genes in essential hypertension
Authors: D.C. Hu, X.L. Zhao, J.C. Shao, W. Wang, J. Qian, A.H. Chen, H.Q. Zhang, H. Guo, J. Jiang and H.Y. Li
We explored the interaction of 6 candidate genetic mutations in essential hypertension (EH). The mutations AGT M235T, ACE I/D, eNOS Glu298Asp, ET-2 A985G, ANP T2238C, and NPRC A-55C were detected using a genechip microarray in 100 patients with EH and 97 controls from the Han population living in the Yunnan Province of China. Risks of EH were evaluated wi.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.14
- DOI:
- 10.4238/2014.October.20.14
Single- and double-SSR primer combined analyses in rice
Authors: J. Ma, S.C. Guan, Z. Zhang and P.W. Wang
Polymerase chain reaction (PCR) is the foundation of SSR molecular marker technology. We used sib rice varieties J518, XD1 and SD23 as experimental materials, selecting 30 pairs of SSR primers, including RM127, RM337 and RM5172, covering the rice genome, and performed single- and double-SSR primer combined analyses. We found t.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.1
- DOI:
- 10.4238/2012.April.27.1
An AFLP-based approach for the identification of sex-linked markers in blunt snout bream, Megalobrama amblycephala (Cyprinidae)
Authors: H.O. Rao, J.C. Deng, W.M. Wang and Z.-X. Gao
Sex-specific DNA markers are useful for studying sex-determination mechanisms and establishment of monosex populations. Three widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in China) of blunt snout bream (Megalobrama amblycephala) were screened with AFLPs to search for sex-linked markers. Female and mal.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.7
- DOI:
- 10.4238/2012.April.19.7
Identification of RNA editing sites in cotton (Gossypium hirsutum) chloroplasts and editing events that affect secondary and three-dimensional protein structures
Authors: Y. Jiang, S.L. Fan, M.Z. Song, J.N. Yu and S.X. Yu
RNA editing can alter individual nucleotides in primary transcripts, which can cause the amino acids encoded by edited RNA to deviate from the ones predicted from the DNA template. We investigated RNA editing sites of protein-coding genes from the chloroplast genome of cotton. Fifty-four editing sites were identified in 27 tra.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.4
- DOI:
- 10.4238/2012.April.19.4
Effects of various salinities on Na+-K+-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis
Authors: M. Sun, K. Jiang, F. Zhang, D. Zhang, A. Shen, M. Jiang, X. Shen and L. Ma
Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0, 2, 5, 10, a.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.3
- DOI:
- 10.4238/2012.April.19.3
MEF2A gene mutations and susceptibility to coronary artery disease in the Chinese population
Authors: J. Li1, H.-X. Chen2, J.-G. Yang3, W. Li3, R. Du3 and L. Tian3
We investigated mutations and polymorphisms of the coronary artery disease (CAD)-related myocyte enhancer factor 2A (MEF2A) gene in a Chinese population. Polymerase chain reaction-single-strand conformation polymorphism and DNA sequencing were used to detect exon 11 of the MEF2A gene in 210 Hubei patients with CAD and 190 healthy controls... Read More»
- Genet. Mol. Res. 13(4):
- /2014.October.20.15
- DOI:
- /10.4238/2014.October.20.15
Correlation between mandibular gland secretion and cuticular hydrocarbons in them stingless bee Melipona quadrifasciata
Authors: C. Cruz-Landim, M.J. Ferreira-Caliman, L.F. Gracioli-Vitti and R. Zucchi
We investigated whether Melipona quadrifasciata worker mandibular gland secretions contribute directly to their cuticular hydrocarbon profile. The mandibular gland secretion composition and cuticular surface compounds of newly emerged worker bees, nurse bees, and foragers were determined by gas chromatography and mass spectrom.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.2
- DOI:
- 10.4238/2012.April.19.2
Cloning and functional identification of a novel BCA3 splice
Authors: Y. Zhang, M.Y. Hu, C.X. Qiao, J.N. Feng, Z. Lin, B.F. Shen2, Y.F. Ma, Y. Li, Y.L. Li and M.
The human breast cancer-associated gene (BCA3) was first discovered in breast and prostate cancer cells lines. In vivo studies have shown that BCA3 is mainly expressed in breast tumor cells and not in normal breast and prostate tissues. To date, 3 splice variants of BCA3 have been reported: a double-absent variant lacking exon 3 and exon 5 (BCA3-1), an ex.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.7
- DOI:
- 10.4238/2014.December.18.7
Quantitative trait loci associated with body weight and abdominal fat traits on chicken chromosomes 3, 5 and 7
Authors: S.Z. Wang, X.X. Hu, Z.P. Wang, X.C. Li, Q.G. Wang, Y.X. Wang, Z.Q. Tang and H. Li
Body weight and abdominal fat traits in meat-type chickens are complex and economically important factors. Our objective was to identify quantitative trait loci (QTL) responsible for body weight and abdominal fat traits in broiler chickens. The Northeast Agricultural University Resource Population (NEAURP) is a cross between b.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.1
- DOI:
- 10.4238/2012.April.19.1