Intermedin protects against myocardial ischemia-reperfusion injury in hyperlipidemia rats
Authors: S.M. Yang1, J. Liu2 and C.X. Li1
Hyperlipidemia is a well-established risk factor for the development of coronary atherosclerosis, while intermedin (IMD) has been identified as a novel calcitonin/calcitonin gene-related peptide family member involved in cardiovascular protection. However, whether IMD protects against hyperlipidemia-associated myocardial ischemia/reperfusion (MI/R) injury.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.7
- DOI:
- 10.4238/2014.October.20.7
Effects of Mycoplasma pneumoniae infection on airway neurokinin-1 receptor expression in BALB/c mice
Authors: H. Zhang1, B. Wei2, Y.X. Shang1, X.Y. Jiao1, L. Wang1, M.B. He3, X.H. Han1 and G.Z. Wang3
The aim of this study was to establish a BALB/c mouse model of Mycoplasma pneumoniae (MP) infection and to explore the expression of neurokinin-1 receptor (NK1-R) in the trachea and lung tissue and changes in its relative content at different time points (on the 3rd, 7th, 14th, 21st, and 30th days after infection) in MP-infected BALB/c mice. Immunohistoch.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.8
- DOI:
- 10.4238/2014.October.20.8
Restriction-ligation-free (RLF) cloning: a high-throughput cloning method by in vivo homologous recombination of PCR products
Authors: Y. Wang, Y. Liu, J. Chen, M.J. Tang, S.L. Zhang, L.N. Wei, C.H. Li and D.B. Wei
In this study, we optimized a restriction-ligation-free (RLF) method to save time and cost of constructing multiple plasmids with the same gene insert, and examined the efficacy of RLF on high-throughput multi-plasmid cloning. This method utilizes the precise DNA repair and recombination systems within Escherichia coli, which .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.19
- DOI:
- 10.4238/2015.October.9.19
Gli1 expression in pancreatic ductal adenocarcinoma and its clinical significance
Authors: Y. Abula, C. Yi, X.-Y. Wang, M. Wang, R.-Y. Qin, Y.-Q. Guo, H. Lin and H.-J. Li
The aim of this study was to explore the correlation between the expression levels of Gli1 and p53 in pancreatic ductal adenocarcinoma (PDAC) and its pathological significance. Immunohistochemistry (IHC) was employed to measure the expression level of Gli1 and p53 in 85 sets of paraffin-embedded PDAC and corresponding para-car.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.21
- DOI:
- 10.4238/2015.October.9.21
Characterization of Riemerella anatipestifer CH-1 gldJ gene and GldJ protein
Authors: B. Yuan1,2, A.C. Cheng1,2,3 and M.S. Wang1,2,3
Riemerella anatipestifer (RA) CH-1, a highly virulent field strain, was isolated and identified by our laboratory. The gldJ gene was conserved in RA, and it had a typical TATA promoter region and AU-rich sequence within the 5' untranslated region. The GldJ protein was an outer-membrane lipoprotein with a signal peptide cleavage site between amino acids 20.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.9
- DOI:
- 10.4238/2014.October.20.9
Expression and significance of the imprinted gene PEG10 in placenta of patients with preeclampsia
Authors: X.Y. Liang, X. Chen, Y.Z. Jin, X.O. Chen and Q.Z. Chen
The aim of this study was to investigate the expression and significance of the imprinted gene PEG10 (paternally expressed gene 10) in preeclampsia placental tissue. Quantitative real-time reverse transcriptase polymerase chain reaction and immunohistochemistry to evaluate mRNA and protein expression and distribution of PEG10 in placental tissues obtained.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.2
- DOI:
- 10.4238/2014.December.18.2
Variability with altitude of major histocompatibility complex-related microsatellite loci in goats from Southwest China
Authors: G.X. E, Y.F. Huang, Y.J. Zhao and R.S. Na
We aimed to use microsatellite BM1258 loci of the major histocompatibility complex (MHC) as an indicator of the influence of genetic diversity of immunity in goats (Dazu Black, Hechuan White, Meigu, and Tibetan goat). In total, 132 animals comprising 50 Dazu Black goats, 24 Hechuan White goats, 34 Meigu goats, and 24 Tibetan goats were examined. Collectiv.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.26
- DOI:
- 10.4238/2015.November.18.26
APOA5 -1131T/C polymorphism and coronary artery disease susceptibility in Chinese population: an updated meta-analysis and review
Authors: J. Zhang, D.G. Wan, H.L. Song and W.G. Zhang
Although many studies have investigated the association of the APOA5 -1131T/C polymorphism with coronary artery disease (CAD), definite conclusions have not been drawn. To understand the effects of the APOA5 -1131T/C polymorphism on the risk of developing CAD, we performed an updated meta-analysis in the Chinese population. Re.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.22
- DOI:
- 10.4238/2015.October.9.22
Cloning and sequence analysis of the coding sequence of �²-actin cDNA from the Chinese alligator and suitable internal reference primers from the �²-actin gene
Authors: H.N. Zhu, S.Z. Zhang, Y.K. Zhou, C.L. Wang and X.B. Wu
β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.4
- DOI:
- 10.4238/2015.October.9.4
Porous tantalum rods for treating osteonecrosis of the femoral head
Authors: Z.H. Liu, W.S. Guo, Z.R. Li, L.M. Cheng, Q.D. Zhang, D.B. Yue, Z.C. Shi, B.L. Wang, W. Sun and N.F. Zhang
This study evaluated the outcomes of using porous tantalum rods for the treatment of osteonecrosis of the femoral head (ONFH). We performed core decompression and inserted porous tantalum implants in 149 patients (168 consecutive hips) with ONFH. Hips had large (65), medium (64), or small (39) lesions; 63 lesions were lateral, 68 were central, and 35 were.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.10
- DOI:
- 10.4238/2014.October.20.10