Effect of progranulin (PGRN) on the proliferation and senescence of cervical cancer cells
Authors: X.Y. Zhang1, Z.X. Pan1, H. Liu1, J.L. Yu1, G.X. Li2, H.Y. Wang3 and M.M. Liu4
We investigated the effect of progranulin (PGRN) expression on the proliferation and senescence of cervical cancer cells. PGRN small interfering RNA (siRNA) was introduced into the SiHa and HeLa cell lines of human cervical carcinoma using liposome-mediated transfection. The expression levels of PGRN in each cell line after transfection of PGRN siRNA were.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.18
- DOI:
- 10.4238/2015.November.13.18
Exceptional material requirement for reproduction in mouse oocytes
Authors: L. Yu1*, S.F. Wang2*, Q.Z. Zhai3*, Y.Q. Yao3, F. Jiang4 and Y.X. Lu1
Limited information on oocytes and fertilization prevents the efficient therapy of patients with infertility. The most important reason for this lack of understanding is a deficiency in research dedicated to oocytes and fertilization. Currently, we are concerned with the role of nutrition in the process of oocyte development to better understand the relat.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.21
- DOI:
- 10.4238/2015.November.13.21
Molecular characterization, chromosome mapping, and expression profile of porcine CDC16
Authors: D. Xu, J. Jiang and H. Ma
Cell division cycle16 (CDC16) is a core component among the eight protein subunits of the anaphase-promoting complex (APC). APC is a cyclin degradation system that governs the exit of cells from mitosis. Not much information is available for CDC16 in pig. In this study, a 2284-bp cDNA of porcine CDC16 was obtained by rapid amp.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.25
- DOI:
- 10.4238/2015.October.19.25
Genetic variability and phylogeny of the 5' long terminal repeat from Brazilian bovine leukemia virus
Authors: C. Hirsch1, M.F. Camargos2, E.F. Barbosa-Stancioli3, A.A. Fonseca J�ºnior2, D.S. Raj�£o1, M.B. Heinemann1, J.K.P. Reis1 and R.C. Leite1
We conducted a phylogenetic analysis of 22 strains of bovine leukemia virus obtained by polymerase chain reaction to amplify a 582-base pair fragment of the transcriptional regulatory region 5' long terminal repeat (LTR). Twenty-two samples of proviral DNA from peripheral blood mononuclear cells containing bovine leukemia virus from naturally infected bov.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.16
- DOI:
- 10.4238/2015.November.18.16
Identification of differently expressed genes in leukemia using multiple microarray datasets
Authors: Z.Y. Zhang, R.Q. Xu, T.J. Guo, M. Zhang, D.X. Li and X.Y. Lu
The purpose of this study was to identify differentially expressed genes and analyze biological processes related to leukemia. A meta-analysis was performed using the Rank Product package of Gene Expression Omnibus datasets for leukemia. Next, Gene Ontology-enrichment analysis and pathway analysis were performed using the Gene Ontology website and Kyoto E.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.9
- DOI:
- 10.4238/2014.December.12.9
Avian sarcoma and leukosis virus gag gene in the Anser anser domesticus genome
Authors: F. Zhu1, H. Jie3, L. Lian2, L.J. Qu2, Z.C. Hou2, J.X. Zheng2, S.Y. Chen1,N. Yang2 and Y.P. Liu1
Endogenous retroviruses are regarded as ideal genetic markers for evolutionary analyses. Birds were some of the initial vertebrates found to contain endogenous retroviruses. However, few studies have investigated the presence and distribution of endogenous retroviruses in goose. In this study, we detected the avian sarcoma and leukosis virus gag gene in t.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.1
- DOI:
- 10.4238/2015.November.18.1
Vascular endothelial growth factor gene polymorphisms and psoriasis susceptibility: a meta-analysis
Authors: Y.H. Lee and G.G. Song
The aim of this study was to explore whether vascular endothelial growth factor (VEGF) polymorphisms confer susceptibility to psoriasis. Meta-analyses were conducted to examine the associations between the +405 C/G, -460 C/T, -1154 A/G, and -2578 A/C polymorphisms of VEGF and psoriasis using allele contrast and recessive, dominant, and addi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.3
- DOI:
- 10.4238/2015.November.18.3
Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers
Authors: L.G. Zhang, Y. Chang, X.F. Zhang, F.Z. Guan, H.M. Yuan, Y. Yu and L.J. Zhao
Hemp (Cannabis sativa) is an important fiber crop, and native cultivars exist widely throughout China. In the present study, we analyzed the genetic diversity of 27 important Chinese native hemp cultivars, by using inter-simple sequence repeats (ISSR) and chromosome markers. We determined the following chromosome formulas: 2n = 20 = 14m + 6sm; 2n = 20 = 2.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.10
- DOI:
- 10.4238/2014.December.12.10
Expression of aquaporin 1 and 4 in rats with acute hypoxic lung injury and its significance
Authors: Y. Wu, C.Y. Pan, C.Z. Guo, Z.J. Dong, Q. Wu, H.M. Dong and W. Zhang
Aquaporin (AQP)-1 and AQP-4 expression in lung tissues of SD rats during high altitude hypoxic lung injury, and the relationship between AQP-1 and AQP-4 expression, and acute hypoxic lung injury was analyzed. Thirty six healthy SD rats were divided into hypoxia 1d, 2d, 3d, 5d, and 7d groups and control group (N = 6). Pathologi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.19
- DOI:
- 10.4238/2015.October.19.19
Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans
Authors: D.R. Cavalcanti, C.B. Malafaia, T.D. Silva, B.S. Santos,G.M.T. Calazans and M.V. Silva
Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.4
- DOI:
- 10.4238/2015.November.18.4