Nested polymerase chain reaction amplification and sequencing analysis of the light-chain and heavy-chain variable regions in the influenza A H1N1 virus hemagglutinin monoclonal antibody gene
Authors: H.J. Li, C.Y. Guo, J.Y. Sun, L.J. Sun, P.H. Zhao, L. Hu, Y. Li and J. Hu
The nested polymerase chain reaction (PCR) method was used for the amplification of the influenza A H1N1 virus hemagglutinin monoclonal antibody light-chain and heavy-chain genes. Sequence analysis of the obtained genes was then used to identify common cloning methods of the mouse immunoglobulin-kappa (Igκ) light-chain a.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.11.1
- DOI:
- http://dx.doi.org/10.4238/2014.June.11.1
Cryopreservation does not alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid mesenchymal stem cells
Authors: P.C. Ã?Â?ngelo, A.C.S. Ferreira, V.D. Fonseca, S.P. Frade, C.S. Ferreira, F.S.V. Malta, A.K. Pereira, H.V. Leite, A.P. Brum, V.C. Pardini, K.B. Gomes and A.C.V. Cabral
Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.5
- DOI:
- 10.4238/2012.April.19.5
Isolation and expression analysis of the soybean GmPic gene
Authors: A.-H. Sha, H. Wu, X.-M. Fu, Q.-L. Zhang, Q.-L. Guo, Y.-H. Chen and X.-A. Zhou
The differential screening method was used to isolate the soy photoperiodic response-related genes and to further elucidate the molecular mechanisms of the soybean photoperiodic response. The light-sensitive species Zhong Dou 24 was used to receive long-time sunshine, short-time sunshine, and natural sunshine treatment. The cD.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.11.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.11.2
Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism
Authors: T. Kanchanaketu, N. Sangduen, T. Toojinda and V. Hongtrakul
Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.13.2
- DOI:
- 10.4238/2012.April.13.2
High methylation of the SEPT9 gene in Chinese colorectal cancer patients
Authors: X.L. Su, Y.F. Wang, S.J. Li, F. Zhang and H.W. Cui
Methylation of the septin 9 gene (SEPT9) occurs in higher frequency in colorectal cancer (CRC) compared to control samples, which suggests that SEPT9 methylation is a useful biomarker for screening CRC. However, the methylation status of SEPT9 in Chinese CRC patients is scarcely reported. In the present study, SEPT9 methylatio.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.17.5
- DOI:
- 10.4238/2014.January.17.5
Population divergence and peculiar karyoevolutionary trends in the loricariid fish Hypostomus aff. unae from northeastern Brazil
Authors: J.A. Bitencourt, P.R.A.M. Affonso, L. Giuliano-Caetano, P.L.S. Carneiro and A.L. Dias
Loricariidae (Siluriformes, Hypostominae) is one of the most diverse catfish families. In spite of the wide distribution of loricariids in South America, cytogenetic reports are available for only a few species, mostly from southern and southeastern Brazil. We made the first chromosomal analysis of Hypostomus aff. unae from th.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.13.1
- DOI:
- 10.4238/2012.April.13.1
Relationship between perioperative cardiovascular risk factors and bone marrow cells from patients undergoing coronary artery bypass grafting surgery
Authors: L. Zhang, R. Wang, C.-S. Xiao, Y. Wu and C.-Q. Gao
Cell therapy through the implantation of autologous bone marrow cells has long been used in clinical trials for the treatment of ischemic heart diseases. However, as the outcomes of cell implantation vary among patients, risk factors that might influence the level and function of bone marrow progenitor cells should be determin.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.11
- DOI:
- 10.4238/2015.November.25.11
Investigation of AZF microdeletions in patients with Klinefelter syndrome
Authors: L.X. Li, H.Y. Dai, X.P. Ding, Y.P. Zhang, X.H. Zhang, H.Y. Ren and Z.Y. Chen
We investigated azoospermia region microdeletions in male infertility patients with Klinefelter syndrome (KFS), as well as the association between azoospermia symptoms in patients with KFS and Y chromosome microdeletion polymorphisms. A total of 111 cases with male infertility confirmed to have KFS (47, XXY) and 94 fertile men.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.2
- DOI:
- 10.4238/2015.November.25.2
1H-MRS before and after resuscitation following selective cerebral ultra-profound hypothermic blood flow occlusion in monkeys
Authors: X.-Q. Niu, X.-X. Zhao, B.-C. Li, Y.-J. Gao, W. Xu, Y.-D. Fan, G.-P. Fu, K. Wang and J. Pu
We investigated the effect of selective cerebral ultra-profound hypothermic blood flow occlusion on brain tissue and cell metabolism to ascertain the efficacy and safety of selective deep hypothermic technologies using proton magnetic resonance spectroscopy (1H-MRS). The bilateral carotid artery was blocked at room temperature.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.3
- DOI:
- 10.4238/2015.October.19.3
Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain
Authors: F.J. Delgado-Martinez, I. Amaya, J.F. S�¡nchez-Sevilla and M.C. Gomez-Jimenez
Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.7
- DOI:
- 10.4238/2012.April.10.7