PpPIF-1: first isolated full-length PIF-like element from the bamboo Phyllostachys pubescens
Authors: M.B. Zhou, X.M. Liu and D.Q. Tang
PIF-like elements are the first-described members of a recently discovered and widespread superfamily of DNA transposons, named PIF/Harbinger. Complete and partial PIF-like elements have been isolated from hundreds of plant species. Previously, we identified 139 partial PIF-like transposases in the Bambusoideae, of which three.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.3.3
- DOI:
- 10.4238/2012.April.3.3
Identification and characterization of polymorphic microsatellite loci in the red-crowned crane
Authors: L. Zhang, Z.H. Zhang, F.J. Shen, R. Hou, W.P. Zhang, Y.L. Liu, K.Y. Tu and A.L. Yang
We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes... Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.5
- DOI:
- 10.4238/2015.November.25.5
Expression of CD44 in sheep oocytes and preimplantation embryos
Authors: J.V. Luz, A.S. Alc�¢ntara-Neto, R.I.T.P. Batista, J.M.G. Souza, D.I.A. Teixeira, L.M. Melo and V.J.F. Freitas
The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35).. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.3.2
- DOI:
- 10.4238/2012.April.3.2
Molecular cloning and expression of the male sterility-related CtYABBY1 gene in flowering Chinese cabbage (Brassica campestris L. ssp chinensis var. parachinensis)
Authors: X.L. Zhang and L.G. Zhang
Expression of the YABBY gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. However, the YABBY gene has not been studied in male sterility and fertility restoration... Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.10.1
- DOI:
- http://dx.doi.org/10.4238/2014.June.10.1
QTLs for days to silking in a recombinant inbred line maize population subjected to high and low nitrogen regimes
Authors: Z.P. Zheng, X.H. Liu, Y.B. Huang, X. Wu, C. He and Z. Li
Days to silking (DTS) is one of the most important traits in maize (Zea mays). To investigate its genetic basis, a recombinant inbred line population was subjected to high and low nitrogen (N) regimes to detect quantitative trait loci (QTLs) associated with DTS. Three QTLs were identified under the high N regime; these explain.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.3.1
- DOI:
- 10.4238/2012.April.3.1
Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis
Authors: W. Zhai, Y. Zhao, L.X. Zhang and X.J. Li
The tomato Pto gene encodes a serine/threonine kinase (STK) whose molecular characterization has provided valuable insights into the disease resistance mechanism of tomato. Therefore, Pto is considered as a promising candidate for engineering broad-spectrum pathogen resistance in this crop. In this study, a pair of degenerate .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.10.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.10.2
miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary
Authors: F. Tu, Z.X. Pan, Y. Yao, H.L. Liu, S.R. Liu, Z. Xie and Q.F. Li
During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.14.6
- DOI:
- 10.4238/2014.January.14.6
Correlation analysis between starch properties and single nucleotide polymorphisms of waxy genes in common rye (Secale cereale L.)
Authors: M. Meng, X. Gao, L.J. Han, X.Y. Li, D. Wu, H.Z. Li and Q.J. Chen
To understand the relationships between single nucleotide polymorphisms (SNPs) in the waxy gene and starch parameters in common rye, we performed sequence characterization, enzyme activity testing, amylopectin/amylose ratio evaluation, starch property testing, and correlation analysis. Specific primers were used to clone waxy .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.14.7
- DOI:
- 10.4238/2014.January.14.7
Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers
Authors: J.B. Qin, Y. Wang and C.Q. Zhu
Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella .. Read More»
- Genet. Mol. Res. 13(2):
Association between methylene tetrahydrofolate reductase and glutathione S-transferase M1 gene polymorphisms and chronic myeloid leukemia in a Brazilian population
Authors: G.S. Lordelo, A.L. Miranda-Vilela, A.K. Akimoto, P.C.Z. Alves, C.O. Hiragi, A. Nonino, M.B. Daldegan, M.N. Klautau-Guimar�£es and C.K. Grisolia
Chronic myeloid leukemia is a hematopoietic stem cell disorder that causes uncontrolled proliferation of white blood cells. Although the clinical and biological aspects are well documented, little is known about individual susceptibility to this disease. We conducted a case-control study analyzing the prevalence of the polymor.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.6
- DOI:
- 10.4238/2012.April.19.6