Absence of the exon 1 coding sequence of the androgen receptor gene associated with teratozoospermia in a Brazilian population

Authors: W.E.J.C. Mesquita, M.S. Approbato, K.K.V.O. Moura, and R.S.A. Jesu�­no

The androgen receptor (AR) is a protein encoded by the AR gene, which when mutated may affect spermatogenesis, the process in which spermatozoa are produced; thus, AR mutations could lead to male infertility. We examined exon 1 of the AR gene in men with idiopathic infertility. Blood or semen samples from 111 infertile, oligoz.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr686

DOI: 10.4238/vol8-4gmr686

Characterization of an endophytic bacterial community associated with Eucalyptus spp

Authors: R.E.L. Proc�³pio, W.L. Ara�ºjo, W. Maccheroni Jr. and J.L. Azevedo

Endophytic bacteria were isolated from stems of Eucalyptus spp (Eucalyptus citriodora, E. grandis, E. urophylla, E. camaldulensis, E. torelliana, E. pellita, and a hybrid of E. grandis and E. urophylla) cultivated at two sites; they were characterized by RAPD and amplified rDNA restriction analysis (ARDRA). Endophytic bacteria.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr691

DOI: 10.4238/vol8-4gmr691

Changes in gene expression profiles of bovine embryos produced in vitro, by natural ovulation, or hormonal superstimulation

Authors: T.C.D. Mundim, A.F. Ramos, R. Sartori, M.A.N. Dode, E.O. Melo, L.F.S. Gomes, R. Rumpf and M.M. Franco

Embryos produced by hormonal superstimulation have been used as an in vivo control in most published research on embryo gene expression. However, it is not known if this is the most appropriate control for gene expression profile studies. We compared the expression of GRB-10, IGF-II, IGF-IIR, MnSOD, GPX-4, catalase, BAX, and i.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr646

DOI: 10.4238/vol8-4gmr646

Genetic diversity of Saccharum officinarum accessions in Pakistan as revealed by random amplified polymorphic DNA

Authors: F.A. Khan, A. Khan, F.M. Azhar and S. Rauf

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clon.. Read More»

Genet. Mol. Res. 8(4):

Protective effects of �²-carotene against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Authors: C.D. Dias, B.C. Ara�ºjo, E.S. Dutra and J.C. Nepomuceno

β-carotene (BC), pro-vitamin A, is an efficient antioxidant, effective in the neutralization of oxygen reactive species, which cause serious damage to DNA. Various studies have been conducted on the effectiveness of BC for chemoprevention of cancer and heart disease. Doxorubicin is a chemotherapeutic agent used for cancer.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr626

DOI: 10.4238/vol8-4gmr626

Association of single nucleotide polymorphisms with carcass traits in Nellore cattle

Authors: J.B.S. Ferraz, L.F.B. Pinto, F.V. Meirelles, J.P. Eler, F.M. de Rezende, E.C.M. Oliveira, H.B. Almeida, B. Woodward4 and D. Nkrumah

The association between two single nucleotide polymorphisms (SNPs), T945M and UCP1SNP1, with hot carcass weight (HCW, kg, N = 618), longissimus dorsi muscle area (REA, cm2, N = 633), and backfat thickness (BF, mm, N = 625), measured in Nellore cattle in Brazil, was evaluated. Likelihood ratio tests were used to evaluate reduce.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr650

DOI: 10.4238/vol8-4gmr650

Genetic diversity and population structure in Brazilian Mangalarga Marchador horses

Authors: J.B. DeAssis, D.M. DeLaat, M.G.C.D. Peixoto, J.A.G. Bergmann,C.G. Fonseca and M.R.S. Carvalho

One hundred and fifteen unrelated Mangalarga Marchador horses were sampled from three geographically distinct regions of Minas Gerais State, Brazil (South, Southeast, and Northeast) and tested for 10 microsatellite loci. Genetic diversity and population structure parameters were estimated with ARLEQUIN 3.0, CERVUS 2.0, POPGENE.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr647

DOI: 10.4238/vol8-4gmr647

Combined detection of p53, p16, Rb, and EGFR mutations in lung cancer by suspension microarray

Authors: Y. Ye, D. Wang, C. Su, T. Rong and A. Guo

Mutations of some contributing factors (p53, p16, Rb, and EGFR) are believed to affect diagnosis and drug resistance of lung cancer. We evaluated the efficacy of a multimarker panel for molecular diagnosis of lung cancer, using a high-throughput suspension microarray. One hundred and twenty-five lung cancer specimens and 30 tu.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr627

DOI: 10.4238/vol8-4gmr627