A.A. Wenceslau

Cytogenetic aspects of a canine breast carcinosarcoma - a case report

• C.S.D. Morais
• P.R.A.M. Affonso
• J.A. Bitencourt
• A.A. Wenceslau

This study searched a rare and aggressive type of cancer in dogs and humans, the breast carcinosarcoma. Both clinical and pathological traits of mammary carcinosarcomas in dogs are similar to humans, such as infrequent occurrence, fast tumor growth, and unfavorable prognosis when compared to carcinomas. Other possible alterations include chromosomal abnormalities th.. Read More»

Genet. Mol. Res. 16(2): gmr16029433

DOI: 10.4238/gmr16029433

Leishmania (Viannia) braziliensis in dogs in Brazil: epidemiology, co-infection, and clinical aspects

F.S. Carvalho, A.A. Wenceslau, G.R. Albuquerque, A.D. Munhoz, E. Gross, P.L.S. Carneiro, H.C. Oliveira, J.M. Rocha, I.A. Santos and R.P. Rezende

Leishmaniasis is an endemic disease present in 98 countries. In Brazil, the northeast region accounts for approximately half of the cases in humans, and has experienced an increased number of positive cases in dogs. In this study, we investigated the epidemiology of canine leishmaniasis in the city of Ilhéus, Bahia, using serological and molecular techniques .. Read More»

Genet. Mol. Res. 14(4): 2015.October.5.19

DOI: 10.4238/2015.October.5.19

Direct preparation protocol to obtain mitotic chromosomes from canine mammary tumors

C.S.D. Morais, P.R.A.M. Affonso, J.A. Bitencourt and A.A. Wenceslau

Currently, mammary neoplasms in female canines are a serious problem in veterinary clinics. In addition, the canine species is an excellent disease model for human oncology because of the biological and genetic similarities between the species. Cytogenetics has allowed further study of the characterization of neoplasms in canines. We hypothesized that the use of a direct preparation protocol fo.. Read More»

Genet. Mol. Res. 14(4): 2015.December.29.49

DOI: 10.4238/2015.December.29.49

Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

F.S. Carvalho, A.A. Wenceslau, M. Teixeira and G.R. Albuquerque

We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the .. Read More»

Genet. Mol. Res. 10(2):