Plant Genetics
Molecular cloning and expression analysis of MyD88 in spiny head croaker, Collichthys lucidus
Authors: C. Sang, Y. Lin, K. Jiang, F. Zhang, C. Ma, L. Ma and W. Song
Myeloid differentiation factor 88 (MyD88) is an important adaptor protein involved in toll-like receptor signaling pathways. In this study, a cDNA library from Collichthys lucidus was constructed using the SMART technique. A complete cDNA sequence showing high identity with the conserved sequence of the MyD88 gene was cloned from the cDNA library using ex.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.26
- DOI:
- 10.4238/2015.May.4.26
Obese gene polymorphism in Pietrain and large white pigs after a divergent selection
Authors: A.C.P. Silveira, R.C. Antunes, J.F. Almeida, T.F. Braga, P.F.A. Freitas, A.S.M. C�©sar and E.C. Guimar�£es
The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fra.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr511
- DOI:
- 10.4238/vol7-4gmr511
Detection of a resistance gradient to Passion fruit woodiness virus and selection of �yellow� passion fruit plants under field conditions
Authors: C.B.M. Cerqueira-Silva, C.N. Moreira, A.R. Figueira, R.X. Corr�ªa and A.C. Oliveira
Productivity of ‘yellow’ passion fruit (Passiflora edulis Sims. f. flavicarpa O. Deg.) is reduced by infection with Cowpea aphid-borne mosaic virus (CABMV). We examined resistance in 72 yellow passion fruit plants grown from open-pollinated commercial seed. Plants were mechanically inoculated with CABMV virus and maintained in the field in ord.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr484
- DOI:
- 10.4238/vol7-4gmr484
Molecular characterization of an expressed sequence tag representing the cuticle-degrading serine protease gene (PII) from the nematophagous fungus Arthrobotrys oviformis by differential display technology
Authors: A. Nagee, A. Acharya, A. Shete, P.N. Mukhopadhyaya and B.A. Aich
The technology of mRNA-based differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) was used to detect a 246-bp differentially expressed fragment from the nematophagous fungus Arthrobotrys oviformis when young mycelia were induced with the round worm Haemonchus contortus. The fragment was converted into an expressed sequence tag (.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr487
- DOI:
- 10.4238/vol7-4gmr487
Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress
Authors: M.R. Naroui Rad M. Abdul Kadir M.Y. Rafii H.Z.E. Jaafar M.R. Naghavi
This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under dr.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.12.5
- DOI:
- 10.4238/2012.November.12.5
Isolation and characterization of microsatellite markers for Axonopus compressus (Sw.) Beauv. (Poaceae) using 454 sequencing technology
Authors: X.-L. Wang, Y. Li, L. Liao, C.-J. Bai and Z.-Y. Wang
Axonopus compressus (Sw.) Beauv. is a perennial herb widely used as a garden lawn grass. In this study, we used Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO, to isolate simple sequence repeat markers from the A. compressus genome. A total of 1942 microsatellite loci were identified, with 53,193 raw sequencing reads. O.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.2
- DOI:
- 10.4238/2015.May.11.2
Expression of genes associated with the biosynthetic pathways of abscisic acid, gibberellin, and ethylene during the germination of lettuce seeds
Authors: A.C.S. Clemente, R.M. Guimar�£es, D.C. Martins, L.A.A. Gomes,F. Caixeta, R.G.E. Reis and S.D.V.F. Rosa
Seed germination and dormancy are complex phenomena that are controlled by many genes and environmental factors. Such genes are indicated by phytohormones that interact with each other, and may cause dormancy or promote seed germination. The objective of this study was to investigate gene expression associated with the biosynthetic pathways of abscisic ac.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.3
- DOI:
- 10.4238/2015.May.11.3
Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers
Authors: Z.-H. Liu, Q. Xie and Z.-Q. Li
Pinus tabulaeformis f. shekanensis is a rare taxon endemic in the Ziwuling Loess Plateau, of which only one population is known. Inter-simple sequence repeat molecular markers were employed to compare the taxon’s genetic diversity with its 4 nearest wild relatives (P. tabulaeformis, P. tabulaeformis var. mukdensis, P. massoniana, and P. henryi) to a.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.7
- DOI:
- 10.4238/2015.February.6.7
Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes
Authors: F. Perecin S.C. M�©o W. Yamazaki C.R. Ferreira G.K.F. Merighe F.V. Meirelles J.M. Garcia
Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and .. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr541
- DOI:
- 10.4238/vol8-1gmr541
Identification of differentially expressed genes associated with flower color in peach using genome-wide transcriptional analysis
Authors: Y. Zhou, X.X. Wu, Z. Zhang and Z.H. Gao
Flower color is an important trait of the ornamental peach (Prunus persica L.). However, the mechanism responsible for the different colors that appear in the same genotype remains unclear. In this study, red samples showed higher anthocyanins content (0.122 ± 0.009 mg/g), which was significantly different from that in white samples (0.066 ±.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.5
- DOI:
- 10.4238/2015.May.11.5