
Authors: V.P.O. Moraes, S.S. Cereali, O. Froehlich, A.L. Dias
We made a cytogenetic study of the fish Rhamdia quelen collected from the Bodoquena Plateau, an isolated national park region in Mato Grosso do Sul State, Brazil. The diploid number was 2n = 58, with 36 metacentric + 16 submetacentric + 6 subtelocentric chromosomes. We found one to three B chromosomes, which were metacentric a.. Read More»
Authors: D.N. Follmann, A. Cargnelutti Filho, L.H. Lorentz, A.A. Boligon,M. Caraffa and C.A. Wartha
The sunflower has adaptability to growing regions with different climatic and soil characteristics, showing drought tolerance and high-quality oil production. The State of Rio Grande do Sul is the third largest sunflower producer in Brazil, with research related to the sunflower breeding initiated after the decade of 1950. The.. Read More»
Authors: H.J. Zhang and F.S. Luan
The aim of the present study was to develop a transformation system that may be useful for introducing agronomically and biotechnologically relevant traits into melon. The production of transplanted melon with maternal inheritance of the transgene could solve problems related to outcrossing between genetically modified crops and conventional crops or thei.. Read More»
Authors: D. Wu, J. Dong, Y.J. Yao, W.C. Zhao and X. Gao
The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, N.. Read More»
Authors: C.J. Yang, G.Y. Li and Y.L. Cui
The transcriptomes of salt-stressed and unstressed Betula kirghisorum plants were analyzed using high throughput sequencing technology. A total of 52,239,804 and 51,772,998 clean reads were obtained from the two libraries, respectively, and de novo assembled into 60,545 all-unigenes. A total of 39,997 unigenes were annotated using public databases. Overal.. Read More»
Authors: X.B. Luo, Z. Liu, L. Xu, Y. Wang, X.W. Zhu, W. Zhang, W. Chen, Y.L. Zhu, X.J. Su, M. Everlyne and L.W. Liu
Glucosinolates (GSLs) are important secondary metabolites in Brassicaceae plants. Previous studies have mainly focused on GSL contents, types, and biosynthesis-related genes, but the molecular characterization patterns of GSL biosynthesis-related transcription factors remain largely unexplored in radish (Raphanus sativus L.). To isolate transcription fact.. Read More»
Authors: N.K. Korir, W. Diao, R. Tao, X. Li, E. Kayesh, A. Li, W. Zhen and S. Wang
The genetic diversity and relationship of 42 tomato varieties sourced from different geographic regions was examined with EST-SSR markers. The genetic diversity was between 0.18 and 0.77, with a mean of 0.49; the polymorphic information content ranged from 0.17 to 0.74, with a mean of 0.45. This indicates a fairly high degree .. Read More»
Authors: H.J. Zhang, P. Gao, X.Z. Wang and F.S. Luan
An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. The new Agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. The transformatio.. Read More»
Authors: Y. Cheng, Z.P. Yao, M.Y. Ruan, Q.J. Ye, R.Q. Wang, G.Z. Zhou, J. Luo, Z.M. Li, Y.J. Yang and H.J. Wan
The WRKY family is one of the most important transcription factor families in plants, involved in the regulation of a broad range of biological roles. The recent releases of whole-genome sequences of pepper (Capsicum annuum L.) allow us to perform a genome-wide identification and characterization of the WRKY family. In this study, 61 CaWRKY proteins were .. Read More»
Authors: J.S. Freitas, E.M. Silva, A. Rossi
The present study was designed to identify nutrient-dependent changes in extracellular pH and acid phosphatase secretion in the biA1 palC4 mutant strain of Aspergillus nidulans. The palC4 mutant was selected as lacking alkaline phosphatase, but having substantially increased acid phosphatase activity when grown on solid minima.. Read More»