Plant Genetics
Genome-wide analysis of the GRAS gene family in physic nut (Jatropha curcas L.)
Authors: Z.Y. Wu, P.Z. Wu, Y.P. Chen, M.R. Li, G.J. Wu and H.W. Jiang
GRAS proteins play vital roles in plant growth and development. Physic nut (Jatropha curcas L.) was found to have a total of 48 GRAS family members (JcGRAS), 15 more than those found in Arabidopsis. The JcGRAS genes were divided into 12 subfamilies or 15 ancient monophyletic lineages based on the phylogenetic analysis of GRAS proteins from both flowering .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.31
- DOI:
- 10.4238/2015.December.29.31
Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp
Authors: F. Liu and Y.P. Cao
The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroAPantoea s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.33
- DOI:
- 10.4238/2015.December.29.33
Assessment of genetic diversity of cucumber cultivars in China based on simple sequence repeats and fruit traits
Authors: Y.T. Yang, Y. Liu, F. Qi, L.L. Xu, X.Z. Li, L.J. Cong, X. Guo, S.X. Chen and Y.L. Fang
The cucumber (Cucumis sativus L.) is an important crop grown worldwide. In this study, the genetic diversity of 42 cucumber cultivars in China was analyzed using 51 pairs of simple sequence repeat (SSR) primers. These primers identified 129 polymorphic loci, 95.6% of which were polymorphic. The mean effective number of alleles, mean Nei’s gene diver.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.10
- DOI:
- 10.4238/2015.December.29.10
An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Authors: R.R. Oliveira, A.J.C. Viana, A.C.E. Re�¡tegui and M.G.A. Vincentz
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.32
- DOI:
- 10.4238/2015.December.28.32
Analysis of specific sequences in mutant rice generated by introduction of exogenous corn DNA
Authors: W.C. Li, S.D. Ji, C.Z. Tian, Z.K. Li, X.C. Wang, H.C. Zhang, Y.L. Sun, X.Z. Shi, T.X. Wang and J.Y. Li
Rice variation induced by the introduction of exogenous DNA has become an important method of improving rice varieties and creating new germplasms. In this study, we transferred maize genomic DNA fragments to the receptor of Nipponbare rice using a modified “pollen-tube pathway” method. Material from mutant rice B1 and B2 were acquired and 14 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.5
- DOI:
- 10.4238/2015.December.29.5
Rutin, quercetin, and free amino acid analysis in buckwheat (Fagopyrum) seeds from different locations
Authors: C.Z. Bai, M.L. Feng, X.L. Hao, Q.M. Zhong, L.G. Tong and Z.H. Wang
In this study, five common buckwheats and nine tartary buckwheats grown at different locations were analyzed for the contents of rutin, quercetin, and amino acids by high-performance liquid chromatography and spectrophotometry. The rutin content was higher than quercetin in buckwheat seeds. Rutin content was in the range from 0.05 (0.05 g per 100 g dry se.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.11
- DOI:
- 10.4238/2015.December.29.11
Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery
Authors: L.Y. Yu, J.J. Bai, J.J. Fan, D.M. Ma, Y.C. Quan and P. Jiang
Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.35
- DOI:
- 10.4238/2015.December.29.35
Repeatability of agronomic traits in Panicum maximum (Jacq.) hybrids
Authors: T.G.S. Braz, D.M. Fonseca, L. Jank, C.D. Cruz and J.A. Martuscello
When evaluating plants, in particular perennial species, it is common to obtain repeated measures of a given trait from the same individual to evaluate the traits’ repeatability in successive harvests. The degree of correlation among these measures defines the coefficient of repeatability, which has been widely utilized in the study of forage traits.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.38
- DOI:
- 10.4238/2015.December.29.38
Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots
Authors: W.C. Li, S.D. Ji, X.C. Wang, Z.K. Li, H.C. Zhang, C.Z. Tian,Y.L. Liuand C.X. Duan
Corn DNA was introduced into dry seeds of rice (cv. ‘YuJing-6’) by ion beam irradiation. Proteinase activities in rice seedling roots were subsequently analyzed by renaturation electrophoresis at pH 4.5, 7.0, and 8.5. Proteinase activity was more pronounced on gels at higher pH. Irradiation of r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.19
- DOI:
- 10.4238/2015.June.29.19
Integrating genomics into Eucalyptus breeding
Authors: Dario Grattapaglia
The advent of high throughput genomic technologies has opened new perspectives in the speed, scale and detail with which one can investigate genes, genomes and complex traits in Eucalyptus species. A genomic approach to a more detailed understanding of important metabolic and physiological processes, which affect tree growth a.. Read More»
- Genet. Mol. Res. 3(3):