Plant Genetics
Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids
Authors: X. Lu, H. Zhou, Y.-B. Pan, C.Y. Chen, J.R. Zhu, P.H. Chen, Y.-R. Li, Q. Cai and R.K. Chen
No information is available on segregation analysis of DNA markers involving both pollen and self-progeny. Therefore, we used capillary electrophoresis- and fluorescence-based DNA fingerprinting together with single pollen collection and polymerase chain reaction (PCR) to investigate simple sequence repeat (SSR) marker segregation among 964 single pollens.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.26
- DOI:
- 10.4238/2015.December.23.26
Study of the genetic diversity and structure of a natural population of Nectandra megapotamica (Spreng.) Mez. using RAPD markers
Authors: L.S. Costa, L.R.S. Reiniger, B.M. Heinzmann, L.P. Amaral and C.M.L. Serrote
Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of whic.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.28
- DOI:
- 10.4238/2015.December.23.28
Genetic relatedness of artichoke (Cynara scolymus L.) hybrids using random amplified polymorphic DNA (RAPD) fingerprinting
Authors: M.A. Sharaf-Eldin, A. Al-Tamimi, P. Alam, S.F. Elkholy and J.R. Jordan
The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.31
- DOI:
- 10.4238/2015.December.23.31
Selection of reference genes for expression analyses of red-fleshed sweet orange (Citrus sinensis)
Authors: T.T. Pinheiro, D.S. Nishimura, F.B. De Nadai, A. Figueira and R.R. Latado
Red-fleshed oranges (Citrus sinensis) contain high levels of carotenoids and lycopene. The growing consumer demand for products with health benefits has increased interest in these types of Citrus cultivars as a potential source of nutraceuticals. However, little is known about the physiology of these cultivars under Brazilian conditions. Transcriptome an.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.32
- DOI:
- 10.4238/2015.December.23.32
Genome-wide prediction of maize single-cross performance, considering non-additive genetic effects
Authors: J.P.R. Santos, H.D. Pereira, R.G. Von Pinho, L.P.M. Pires, R.B. Camargos and M. Balestre
The prediction of single-cross hybrids in maize is a promising technique for optimizing the use of financial resources in a breeding program. This study aimed to evaluate Genomic Best Linear Unbiased Predictors models for hybrid prediction and compare them with the Bayesian Ridge Regression, Bayes A, Bayesian LASSO, Bayes C, Bayes B, and Reproducing Kerne.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.35
- DOI:
- 10.4238/2015.December.23.35
Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers
Authors: S. Ghariani, H. Elazreg, N. Chtourou-Ghorbel, M. Chakroun and N. Trifi-Farah
Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic d.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.40
- DOI:
- 10.4238/2015.December.23.40
ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding
Authors: A.G.R. Sousa, M.M. Souza, C.A.F. Melo and G.A. Sodr�©
Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 33.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.41
- DOI:
- 10.4238/2015.December.23.41
Comparison of BLUE and BLUP/REML in the selection of clones and families of potato (Solanum tuberosum)
Authors: C.A. Ticona-Benavente and D.F. da Silva Filho
The use of best linear unbiased prediction/restricted maximum likelihood (BLUP/REML) in perennial crops and animal breeding enhances selection gain. However, its advantage with respect to annual crops is not clear. We compared the BLUP and best linear unbiased estimator selection efficiency in the breeding of various potato generations. This was done by s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.30
- DOI:
- 10.4238/2015.December.23.30
Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue
Authors: L. Liu, C.L. Wang, W.Y. Peng, J. Yang, M.Q. Lan, B. Zhang, J.B. Li, Y.Y. Zhu and C.Y. Li
Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimeth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.1
- DOI:
- 10.4238/2015.December.28.1
Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP
Authors: Y. Zhang, X. Yang, F.Y. Zhou, A.F. Zhang, X.F. Zhu, Y. Chen, M.G. Zhou and T.C. Gao
The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.6
- DOI:
- 10.4238/2015.December.28.6