Plant Genetics
Correspondence between performance of Eucalyptus spp trees selected from family and clonal tests
Authors: C.A.F. Reis, F.M.A. Gon�§alves, L.N. Rosse, R.R.G.F. Costa and M.A.P. Ramalho
We examined the correspondence in performance between trees selected from a family test and their respective clones from a clonal test of Eucalyptus. Full-sib families were obtained from controlled pollination among individuals of Eucalyptus grandis and between E. grandis and E. urophylla. The hybridizations did not follow a f.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1078
- DOI:
- 10.4238/vol10-2gmr1078
Single nucleotide polymorphism analysis of the endopolygalacturonase gene in peach and its potential use in crossbreeding programs
Authors: Y. Jiao,R. Ma and M. Yu
Single nucleotide polymorphisms (SNPs) are the most abundant sequence variations found in plant genomes and are widely used as molecular genetic markers in genetic diversity studies and crossbreeding programs. In this study, we examined 113 DNA sequences of the endopolygalacturonase (endo-PG) gene from 67 peach accessions and found a total of 56 SNPs and .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.24
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.24
Genetic differentiation in Aspidosperma polyneuron (Apocynaceae) over a short geographic distance as assessed by AFLP markers
Authors: J.O. Damasceno, E.A. Ruas, L.A. Rodrigues, C.F. Ruas, E. Bianchini, J.A. Pimenta3 and P.M. Ruas
Studies on intraspecific variation can contribute to the development of conservation strategies by identifying units of conservation for threatened species. Aspidosperma polyneuron is a tropical tree of seasonal semideciduous forests that is currently endangered and protected because it has been heavily logged for lumber, alth.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1126
- DOI:
- 10.4238/vol10-2gmr1126
Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum
Authors: M.B. Oliveira, S.C. Barbosa and S. Petrofeza
Dry bean (Phaseolus vulgaris L.) is an important economic crop in Brazil but its yield can be significantly reduced by white mold, a disease caused by Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic, highly destructive, and non-host-specific fungus. This fungus secretes numerous cell wall-degrading enzymes such as polygalacturonases, exo-β-1,.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.6.3
- DOI:
- 10.4238/2013.June.6.3
Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species
Authors: X.Y. Du, Q.N. Hu, Q.L. Zhang, Y.B. Wang and Z.R. Luo
Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-c.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.6.2
- DOI:
- 10.4238/2013.June.6.2
Identification and isolation of full-length cDNA sequences by sequencing and analysis of expressed sequence tags from guarana (Paullinia cupana)
Authors: L.C. Figueir�ªdo, A.C. Faria-Campos, S. Astolfi-Filho and J.L. Azevedo
The current intense production of biological data, generated by sequencing techniques, has created an ever-growing volume of unanalyzed data. We reevaluated data produced by the guarana (Paullinia cupana) transcriptome sequencing project to identify cDNA clones with complete coding sequences (full-length clones) and complete s.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1124
- DOI:
- 10.4238/vol10-2gmr1124
Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco
Authors: A.M. Ding, F.X. Li, Y.Q. Chen,, P. Zong, X. Qu, D.P. Gong, G.S. Liu and Y.H. Sun
As a model plant, mechanisms of the cytoplasmic male sterility/restoration of fertility (CMS/Rf) system in tobacco are seldom studied. Using Rf gene sequences from other Solanaceae plants and the draft genome of Nicotiana benthamiana, degenerate primers were designed to amplify the cDNA pool of N. tomentosiformis. In total, si.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.11
- DOI:
- 10.4238/2014.March.31.11
Development of universal primers for isolating fragments of the LEAFY gene
Authors: L.X. Yu and B. Yan
LEAFY orthologs are found throughout land plants. Degenerate primers were designed for amplifying exon 3 region fragments of the LEAFY gene. Sixteen taxa from different plants were sampled, all of which were successfully isolated, which indicated that this set of primers is universally applicable for all land plants. With the use of this method, there may.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.6.1
- DOI:
- 10.4238/2013.June.6.1
Method for obtaining high-resolution proteomic analysis from pericarps of guarana
Authors: A.L. Souza, P.C.S. Angelo, P.P.O. Nogueira, J.F.C. Gon�§alves, A.M. Franco, S. Astolfi-Filho, J.L. L�³pez-Lozano and E.V. Andrade
Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic path.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.14
- DOI:
- 10.4238/2014.September.29.14
Genetic diversity in wild sweet cherries (Prunus avium) in Turkey revealed by SSR markers
Authors: S. Ercisli, G. Agar, N. Yildirim, B. Duralija, A. Vokurka and H. Karlidag
Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the num.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1196
- DOI:
- 10.4238/vol10-2gmr1196