Plant Genetics
Elucidation of thermotolerance diversity in cotton (Gossypium hirsutum L.) using physio-molecular approaches
Authors: R.M. Rana, S.H. Khan, Z. Ali, A.I. Khan and I.A. Khan
Cotton (Gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. This problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. It is very important to make a comprehensive screening of hea.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1180
- DOI:
- 10.4238/vol10-2gmr1180
Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system
Authors: M.L. Liao,Z.S. Peng,Z.J. Yang,S.H. Wei and P. Martinek
HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed gen.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.14
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.14
Altered callose deposition during embryo sac formation of multi-pistil mutant (mp1) in Medicago sativa
Authors: H.C. Zhou, L. Jin, J. Li and X.J. Wang
Whether callose deposition is the cause or result of ovule sterility in Medicago sativa remains controversial, because it is unclear when and where changes in callose deposition and dissolution occur during fertile and sterile embryo sac formation. Here, alfalfa spontaneous multi-pistil mutant (mp1) and wild-type plants were u.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027968
- DOI:
- 10.4238/gmr.15027968
Genetic diversity in mutated and non-mutated rice varieties
Authors: M. Naeem, F. Ghouri, M.Q. Shahid, M. Iqbal, F. S. Baloch, L. Chen, S. Allah, M. Babar and M. Rana
We studied the genetic variability due to mutation induced by γ-rays (10, 15, and 20 Kr) on various traits of twelve rice genotypes. Mutated and non-mutated seeds were sown in the field between July 2013 and 2014 using a split plot design. Yield and yield-related trait data was recorded, which showed significant (P < 0.05) genotypic and irradiati.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.11
- DOI:
- 10.4238/2015.December.16.11
Microarray profiles of ex vivo expanded hematopoietic stem cells show induction of genes involved in noncanonical Wnt signaling
Authors: D.L. Zanette, J.C.C. Lorenzi, R.A. Panepucci, A.R.D. Santos, G.A. Molfetta, A.G. Araujo, W.A. Silva Junior and M.A. Zago
The low number of hematopoietic stem cells (HSC) in umbilical cord blood (UCB) is directly related to increased risk of transplant failure. Effective ex vivo expansion of HSC has been tried for many years, with conflicting results because of the inability to reproduce in vitro HSC proliferation in the same way it occurs in vivo. We compared freshly isolat.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.15.1
- DOI:
- 10.4238/2013.May.15.1
Isolation and characterization of microsatellite markers for Osmanthus fragrans (Oleaceae) using 454 sequencing technology
Authors: W.J. Yuan, Y. Li, Y.F. Ma, Y.J. Han and F.D. Shang
Osmanthus fragrans (Oleaceae) is an evergreen shrub or small tree that grows in south China. In this study, Roche 454 FLX+ sequencing combined with the magnetic bead enrichment method was used to isolate microsatellite markers from the genome of O. fragrans. A total of 1471 microsatellites that contained enough flanking sequences for primer pair design we.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.15
- DOI:
- 10.4238/2015.December.16.15
Study of genetic variation of eggplant cultivars by using RAPD-PCR molecular markers and the relationship with Phomopsis blight disease reaction
Authors: H.A. Asad, M.B. Meah, S.N. Begum, M.I. Khalil, M.Y. Rafii and M.A. Latif
Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ran.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.1
- DOI:
- 10.4238/2015.December.16.1
Expression of Magnaporthe oryzae genes encoding cysteine-rich proteins secreted during nitrogen starvation and interaction with its host, Oryza sativa
Authors: J. Yang, M.L. Liang, J.L. Yan, Y.Q. Yang, L. Liu, C. Liu, L.J. Yang and C.Y. Li
Previous studies have shown that the blast fungus, Magnaporthe oryzae, may experience nitrogen starvation during infection of its plant host (rice,Oryza sativa). Here, we studied the expression of seven genes encoding cysteine-rich proteins with N-terminal signal peptides during nitrogen limitation and throughout the infection process. Some genes were upr.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.10
- DOI:
- 10.4238/2015.December.16.10
Development and characterization of microsatellite loci for genetic studies of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)
Authors: V.A.C. Pavinato, K.L. Silva-Brand�£o, M. Monteiro, M.I. Zucchi, J.B. Pinheiro, F.L.F. Dias and C. Omoto
We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on averag.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.14.3
- DOI:
- 10.4238/2013.May.14.3
Influence of GSTM1 and GSTT1 polymorphisms on the survival rate of patients with malignant glioma under perillyl alcohol-based therapy
Authors: M.M. Silva, C.O. Da Fonseca, R. Moura-Neto, J.F. Carvalho, T. Quirico-Santos and M.G. Carvalho
GSTM1 (glutathione S-transferase mu 1) and GSTT1 (glutathione S-transferase theta 1) are critical enzymes for detoxification of endogenous and environmental carcinogens. Constitutive GST gene polymorphisms may be associated with increased risk for cancer development. We made an explorative study of a Brazilian population with malignant glioma to determine.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.14.2
- DOI:
- 10.4238/2013.May.14.2