Plant Genetics
Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance
Authors: X.X. Wu, J. Li, X.D. Wu1, Q. Liu, Z.K. Wang, S.S. Liu, S.N. Li, Y.L. Ma, J. Sun, L. Zhao, H.Y. Li, D.M. Li, W.B. Li and A.Y. Su
Drought and salt stresses are the two major factors influencing the yield and quality of crops worldwide. Na+(K+)/H+ antiporters (NHXs) are ubiquitous membrane proteins that play important roles in maintaining the cellular pH and Na+(K+) homeostasis. The model plant Arabidopsis potentially encodes six NHX genes, namely AtNHX1 .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027483
- DOI:
- 10.4238/gmr.15027483
Initial assessment of natural diversity in Mexican fig landraces
Authors: M.T. Garc�?a-Ruiz, V.M. Mendoza-Castillo, E. Valadez-Moctezuma and A. Muratalla-L�?ºa
The common fig (Ficus carica L.) was introduced into Mexico by Spanish Franciscan missionaries in the 16th century. It is widely assumed that Mexican figs are the Spanish cultivar Black Mission. We collected and propagated 12 fig plants from six landraces from different states in Central Mexico that represent different climate. All of them were grown in a.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.12
- DOI:
- 10.4238/2013.September.23.12
Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid nitrogen
Authors: C.Y. Michel-L�?³pez, D. Gonz�?¡lez-Mendoza and O. Grimaldo-Juarez
The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of li.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.10
- DOI:
- 10.4238/2013.September.27.10
DNA barcoding of populations of Fallopia multiflora, an indigenous herb in China
Authors: X.Q. Sun, M.M. Bai, H. Yao, J.L. Guo, M.M. Li and Y.Y. Hang
Fallopia multiflora, locally known as Heshouwu, is one of the most important and widely used Chinese medicinal herbs. However, there is still considerable confusion concerning its different provenances. DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plant species. We assessed the applicab.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.9
- DOI:
- 10.4238/2013.September.27.9
Construction of an integrated genetic map for Capsicum baccatum L.
Authors: M.M. Moulin, R. Rodrigues, H.C.C. Ramos, C.S. Bento, C.P. Sudr�?©, L.S.A. Gon�?§alves and A.P. Viana
Capsicum baccatum L. is one of the five Capsicum domesticated species and has multiple uses in the food, pharmaceutical and cosmetic industries. This species is also a valuable source of genes for chili pepper breeding, especially genes for disease resistance and fruit quality. However, knowledge of the genetic structure of C. baccatum is limited. A refer.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.12
- DOI:
- 10.4238/2015.June.18.12
Improving plant transformation using Agrobacterium tumefaciens
Authors: L.V. Ribeiro Neto, A.P. Oliveira, M.V. Louren�?§o, B.W. Bertoni, S.C. Fran�?§a, T.M. Rosa-Santos and S.M. Zingaretti
Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacte.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.13
- DOI:
- 10.4238/2015.June.18.13
QTLs for rice flag leaf traits in doubled haploid populations in different environments
Authors: J. Cai, M. Zhang, L.B. Guo, X.M. Li, J.S. Bao and L.Y. Ma
Two rice doubled haploid (DH) populations derived from the crosses of ZYQ8/JX17 and CJ06/TN1 were used to detect quantitative trait loci (QTLs) for flag leaf length (FLL), width (FLW), and angle (FLA) under long-day conditions in Hangzhou (subtropical zone) and short-day conditions in Hainan (tropical zone), China. The four parents differed significantly .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.21
- DOI:
- 10.4238/2015.June.18.21
Identification of smut-responsive genes in sugarcane using cDNA-SRAP
Authors: N. Huang, Y.Y. Zhang, X.H. Xiao, L. Huang, Q.B. Wu, Y.X. Que and L.P. Xu
Sugarcane smut, caused by the fungus Sporisorium scitamineum, is one of the main diseases that affect sugarcane worldwide. In the present study, the cDNA-SRAP technique was used to identify genes that are likely to be involved in the response of sugarcane to S. scitamineum infection. In total, 21 bands with significant differential expression during cDNA-.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.23
- DOI:
- 10.4238/2015.June.18.23
Cloning and expression of an APETALA1-like gene from Nelumbo nucifera
Authors: D.Z. Kong, X.Y. Shen, B. Guo, J.X. Dong, Y.H. Li and Y.P. Liu
The objective of this study was to clone the full-length cDNA of the APETALA1 (AP1) gene from lotus and analyze its sequence and expression pattern. The full-length cDNA sequence of the NnAP1 gene was amplified from the petals of Nelumbo nucifera ‘Hongxia’ using RT-PCR and rapid amplification of cDNA ends. Bioinformatic methods were used to an.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.24
- DOI:
- 10.4238/2015.June.18.24
Genetic analysis of water-deficit response traits in maize
Authors: M. Ahmad, M. Saleem, M. Ahsan and A. Ahmad
A set of sixty inbred lines of maize (Zea mays L.) were screened in the greenhouse at the seedling stage under both normal and water-deficit conditions. Six water deficit-tolerant inbred lines were selected based on root to shoot ratios. These selected lines were crossed in a diallel pattern. The parental, F1, and reciprocal c.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017459
- DOI:
- 10.4238/gmr.15017459