Abstract

Improving plant transformation using Agrobacterium tumefaciens

Author(s): L.V. Ribeiro Neto, A.P. Oliveira, M.V. Louren�§o, B.W. Bertoni, S.C. Fran�§a, T.M. Rosa-Santos and S.M. Zingaretti

Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in Eclipta alba explants.

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