Plant Genetics
Evaluation of wheat landrace genotypes for salinity tolerance at vegetative stage by using morphological and molecular markers
Authors: A. Shahzad, M. Ahmad, M. Iqbal, I. Ahmed and G.M. Ali
Identification of new sources of salt tolerance is particularly important to develop crop varieties suitable for saline soils. We evaluated 129 Pakistani and 58 exotic wheat landraces/cultivars grown in Hoagland’s hydroponic nutrient solution, under control (tap water equivalent to 10 mM salt) and salt stress (200 mM NaC.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.19.2
- DOI:
- 10.4238/2012.March.19.2
Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes
Authors: M. Guo, Y.L. Zhang, Z.J. Meng and J. Jiang
Micro-Tom is the smallest known variety of tomatoes. An orthogonal experimental design L16 (45) was used to optimize Agrobacterium-mediated transformation of cotyledon explants of Lycopersicon esculentum cv. Micro-Tom. Four parameters were investigated to determine their effect on transformation frequency: the concentration of.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.16.4
- DOI:
- 10.4238/2012.March.16.4
Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa)
Authors: Y. Wang, B. Bi, Q.H. Yuan, X.L. Li and J.M. Gao
To identify amplified fragment length polymorphism (AFLP) markers associated with resistance or susceptibility of alfalfa to common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae), bulked segregant analysis was conducted based on an F1(M × M) population of 93 plants and a BC1S population of 91.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.14.4
- DOI:
- 10.4238/2012.March.14.4
Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers
Authors: I. Ullah, A. Iram, M.Z. Iqbal, M. Nawaz, S.M. Hasni and S. Jamil
The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.14.3
- DOI:
- 10.4238/2012.March.14.3
Analysis of quantitative trait loci for main plant traits in soybean
Authors: D. Yao, Z.Z. Liu, J. Zhang, S.Y. Liu, J. Qu, S.Y. Guan, L.D. Pan,D. Wang, J.W. Liu and P.W. Wang
Plant traits are important indices for regulating and controlling yield ability in soybean varieties. It is important to comprehensively study the quantitative trait locus (QTL) mapping for soybean plant traits, cloning related genes, and marker assistant breeding. In this study, 236 F2 generation plants and a derivative group were constructed by using J.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.8
- DOI:
- 10.4238/2015.June.8.8
rDNA ITS sequences among morphotypes of Keratell cochlearis, Keratell quadrata and Brachionus forficula (Rotifera)
Authors: Y.L. Ge, Y.L. Xi, J. Ma and D.D. Xu
Morphological variation in rotifers is affected by environmental conditions, making it hard to identify some rotifer taxa. We examined the rDNA ITS sequences of 10 unspined (KCU1-KCU10) and 17 spined (KCS1-KCS17) Keratell cochlearis clones, 26 two-spined (KQT1-KQT26), 18 single-spined (KQS1-KQS18) and 9 unspined (KQU1-KQU9) K... Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.22.7
- DOI:
- 10.4238/2012.March.22.7
Isolation of high quality and polysaccharide-free DNA from leaves of Dimorphandra mollis (Leguminosae), a tree from the Brazilian Cerrado
Authors: H.A.V. Souza, L.A.C. Muller, R.L. Brand�£o and M.B. Lovato
Dimorphandra mollis (Leguminosae), known as faveiro and fava d’anta, is a tree that is widely distributed throughout the Brazilian Cerrado (a savanna-like biome). This species is economically valuable and has been extensively exploited because its fruits contain the flavonoid rutin, which is used to produce medications f.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.22.6
- DOI:
- 10.4238/2012.March.22.6
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.)
Authors: M.F. Santos, K.J. Damasceno-Silva, M.A. Carvalhaes and P.S.C. Lima
The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using randomamplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.10
- DOI:
- 10.4238/2015.June.8.10
Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants
Authors: L.M. Magdalena, E.C. Coipan, A.F. Vladimirescu, L. Savu, M. Costache and L. Gavrila
A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative long-term imprinted/acquired heat shock protein response due to unfriendl.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.22.4
- DOI:
- 10.4238/2012.March.22.4
Genetic variation in a wild population of the �sleep� passion fruit (Passiflora setacea) based on molecular markers
Authors: C.B.M. Cerqueira-Silva, E.S.L. Santos, L.D.H.C.S. Concei�§�£o, C.B. Cardoso-Silva, A.S. Pereira, A.C. Oliveira and R.X. Corr�ªa
Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the ‘sleep’ passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the comple.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.22.3
- DOI:
- 10.4238/2012.March.22.3