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Plant Genetics

Plant Genetics   Research Article

Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations

Authors: H.R. Ning, J.L.Wang, S.Y. Qin, S.Y. Huang, Z.L. Lou, L.Y. Hu and X.Q. Zhu

Toxoplasma gondii, an opportunistic protozoan parasite, infects almost all warm-blooded animals. In this study, we examined the sequence variation in rhoptry protein 20 (ROP20) genes among 18 T. gondii isolates collected from different hosts and geographical regions. Full length ROP20 genes were amplified and sequenced. The results showed that the genes w.. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.8
DOI:
10.4238/2015.July.28.8
Plant Genetics   Research Article

Comparison of efficiency of distance measurement methodologies in mango (Mangifera indica) progenies based on physicochemical descriptors

Authors: E.O.S. Alves, C.B.M. Cerqueira-Silva, A.M. Souza, C.A.F. Santos, F.P. Lima Neto and R.X. CorrĂ?ÂȘa

We investigated seven distance measures in a set of observations of physicochemical variables of mango (Mangifera indica) submitted to multivariate analyses (distance, projection and grouping). To estimate the distance measurements, five mango progeny (total of 25 genotypes) were analyzed, using six fruit physicochemical descr.. Read More»

Genet. Mol. Res. 11(1):
2012.March.14.2
DOI:
10.4238/2012.March.14.2
Plant Genetics   Research Article

Comparison of five DNA extraction methods for molecular analysis of Jerusalem artichoke (Helianthus tuberosus)

Authors: T. Mornkham, P.P. Wangsomnuk, P. Wangsomnuk, S. Jogloy, A. Pattanothai and Y.B. Fu

DNA extraction is an essential step for molecular analysis of an organism, but it is difficult to acquire a sufficient amount of pure DNA from plant tissue with high levels of phenolic compounds, carbohydrates, proteins, and secondary metabolites. Jerusalem artichoke (Helianthus tuberosus) has high levels of such substances. W.. Read More»

Genet. Mol. Res. 11(1):
2012.March.8.5
DOI:
10.4238/2012.March.8.5
Plant Genetics   Research Article

Genetic diversity of Brazilian and introduced olive germplasms based on microsatellite markers

Authors: A.D.B. do Val, J.L. Ferreira, J. Vieira Neto, M. Pasqual, A.F. de Oliveira, A. Bor�©m and G.M.A. Can�§ado

Olive trees have been grown since the beginning of civilization, and the consumption of olives and olive products is increasing worldwide, due to their health benefits and organoleptic qualities. To meet the growing market for olives, commercial cultivation of this species is expanding from traditional areas to new regions. Al.. Read More»

Genet. Mol. Res. 11(1):
2012.March.8.4
DOI:
10.4238/2012.March.8.4
Plant Genetics   Research Article

Physical localization of molecular markers and assignment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) by primed in situ labeling

Authors: Y. Wang, J.F. Wang, H. Yin, H.Q. Gao, N.S. Zhuang and J.P. Liu

Physical localization of molecular markers and assignment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) were achieved using primed in situ labeling. Amplified signals for both the EST507-1 and SSRY13-5 markers were consistently observed in different stages of cell division. A comparison of the length, ar.. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.3
DOI:
10.4238/2015.July.28.3
Plant Genetics   Research Article

Genetic diversity in populations of Acrocomia aculeata (Arecaceae) in the northern region of Minas Gerais, Brazil

Authors: D.A. Oliveira, A.F. Melo JĂ?Âșnior, M.M. BrandĂ?ÂŁo, L.A. Rodrigues, E.V. Menezes and P.R.B. Ferreira

Macaúba (Acrocomia aculeata) is a palm of economic importance, widely distributed in natural forests from Mexico to Uruguay. We analyzed the genetic diversity of populations of macaúba (A. aculeata) in the northern region of the State of Minas Gerais, Brazil. Young leaves from 10 macaúba individuals encomp.. Read More»

Genet. Mol. Res. 11(1):
2012.March.8.1
DOI:
10.4238/2012.March.8.1
Plant Genetics   Research Article

Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes

Authors: S. Ijaz, I.A. Rana, I.A. Khan and M. Saleem

A good culture system provides considerable quantities of highly regenerable target tissues. Embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. Effective exploitation of genetic transformation requires good regeneration systems. We selected three s.. Read More»

Genet. Mol. Res. 11(1):
2012.March.6.4
DOI:
10.4238/2012.March.6.4
Plant Genetics   Research Article

Genetic diversity and differentiation in Camellia reticulata (Theaceae) polyploid complex revealed by ISSR and ploidy

Authors: B.-Y. Wang and Z.-Y. Ruan

Camellia reticulata is a well-known ornamental and oil plant that is endemic to southwest China. This species shows three cell ploidies, i.e., diploidy, tetraploidy and hexaploidy. We made the first investigation of genetic diversity and differentiation of natural populations of C. reticulata, and 114 individuals from 6 popula.. Read More»

Genet. Mol. Res. 11(1):
2012.March.6.3
DOI:
10.4238/2012.March.6.3
Plant Genetics   Research Article

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

Authors: G. Yu, Y.X. Ma, J.-A. Duan, B.S. Song and Z.Q. He

Angelica sinensis is a highly valued medicinal herb, known as female ginseng that is widely cultivated in China. Although A. sinensis is in great demand due to its multiple medicinal and food applications, its early bolting rate (almost 40%) seriously affects crop quality. To better understand its flowering mechanism, cDNA-amp.. Read More»

Genet. Mol. Res. 11(1):
2012.March.6.2
DOI:
10.4238/2012.March.6.2
Plant Genetics   Research Article

Polymorphisms in different EST-SSR types derived from the Chinese bayberry (Myrica rubra, Myricaceae) transcriptome

Authors: S.Y. Zhang, C. Feng, C.J. Xu, C.Q. Zhu and K.S. Chen

Most plant expressed sequence tag-simple sequence repeats (EST-SSRs) are not polymorphic, and it is important to learn the characteristics of highly polymorphic EST-SSRs. In this study, 357 compound and 5557 non-compound EST-SSRs, identified from the transcriptome of the Chinese bayberry (Myrica rubra ‘Biqi’), were divided into 11 types based .. Read More»

Genet. Mol. Res. 14(2):
2015.June.1.21
DOI:
10.4238/2015.June.1.21