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Plant Genetics

Plant Genetics   Research Article

Genome wide cloning of maize meiotic recombinase Dmc1 and its functional structure through molecular phylogeny

Authors: F. Etedali, B. Baghban Kohnehrouz, M. Valizadeh, A. Gholizadeh and M.A. Malboobi

The development of meiotic division and associated genetic recombination paved the way for evolutionary changes. However, the secondary and tertiary structure and functional domains of many of the proteins involved in genetic recombination have not been studied in detail. We used the human Dmc1 gene product along with secondar.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1338
DOI:
10.4238/vol10-3gmr1338
Plant Genetics   Research Article

Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean

Authors: J. Ma, S.C. Guan, D. Yao, Y.F. Wei and P.W. Wang

Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products. We.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1366
DOI:
10.4238/vol10-3gmr1366
Plant Genetics   Review Article

Multiple Mantel tests and isolation-bydistance, taking into account long-term historical divergence

Authors: Mariana Pires de Campos Telles and Jos�© Alexandre Felizola Diniz-Filho

Mantel tests of matrix correspondence have been widely used in population genetics to examine microevolutionary processes, such as isolation-by-distance (IBD). We used partial and multiple Mantel tests to simultaneously test long-term historical effects and current divergence and equilibrium processes, such as IBD. We used the.. Read More»

Genet. Mol. Res. 4(4):
Plant Genetics   Original Articles

A modified method for high-quality DNA extraction for molecular analysis in cereal plants

Authors: Z.H. Pervaiz, N.A. Turi, I. Khaliq, M.A. Rabbani and S.A. Malik

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1346
DOI:
10.4238/vol10-3gmr1346
Plant Genetics   Research Article

Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus

Authors: R. Binyamin, M. Aslam Khan, A.I. Khan, M. Azam Khan, F.S. Awan and N.A. Khan

Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1446
DOI:
10.4238/vol10-3gmr1446
Plant Genetics   Short communication

Meiotic behavior and pollen viability in Moringa oleifera (Moringaceae) cultivated in southern Brazil

Authors: N. Silva, A.B. Mendes-Bonato, J.G.C. Sales and M.S. Pagliarini

Although originally from India, Moringa oleifera is now cultivated throughout most of the tropics, including Brazil. Despite its multipurpose value for food and traditional medicine, little is known about the meiotic behavior and pollen viability of M. oleifera. We evaluated microsporogenesis and pollen viability in eleven pla.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1490
DOI:
10.4238/vol10-3gmr1490
Plant Genetics   Research Article

Analysis of differential selective forces acting on the coat protein (P3) of the plant virus family Luteoviridae

Authors: Marina W. Torres, R�©gis L. Corr�ªa and Carlos G. Schrago

The coat protein (CP) of the family Luteoviridae is directly associated with the success of infection. It participates in various steps of the virus life cycle, such as virion assembly, stability, systemic infection, and transmission. Despite its importance, extensive studies on the molecular evolution of this protein are lack.. Read More»

Genet. Mol. Res. 4(4):
Plant Genetics   Research Article

Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauri�¨s, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes

Authors: Kleber Agari Campos and Carmem Silvia Fontanetti

Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding patte.. Read More»

Genet. Mol. Res. 4(4):
Plant Genetics   Research Article

Dynamic QTL analysis of seed reserve utilization in sh2 sweet corn germination stages

Authors: X.X. Cheng, S. He1 and G.H. Geng

In this study, dynamic quantitative trait loci (QTL) were mapped in a recombinant inbred line (F2:4) population derived from a BF3109 x Q267 cross. This was done during the unconditional (4, 7, and 10 days) and conditional (0 to 4, 4 to 7, and 7 to 10 days) germination stages in sh2 sweet corn. The values of seedling dry weight, weight of mobilized seed r.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038183
DOI:
10.4238/gmr.15038183
Plant Genetics   Research Article

Allelic database and accession divergence of a Brazilian mango collection based on microsatellite markers

Authors: I.C.N. dos Santos Ribeiro F.P. Lima Neto C.A.F. Santos

Allelic patterns and genetic distances were examined in a collection of 103 foreign and Brazilian mango (Mangifera indica) accessions in order to develop a reference database to support cultivar protection and breeding programs. An UPGMA dendrogram was generated using JaccardâÂ?Â?s coefficients from a distance matrix based on 50 alleles of 12 microsatellite.. Read More»

Genet. Mol. Res. 11(4):
2012.October.9.4
DOI:
10.4238/2012.October.9.4