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Plant Genetics

Plant Genetics   Research Article

Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

Authors: P.P. Batista, J.F. Santos, N.T. Oliveira, A.P.D. Pires, C.M.S. Motta and E.A. Luna-Alves Lima

The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification.. Read More»

Genet. Mol. Res. 7(3):
vol7-3gmr422
DOI:
10.4238/vol7-3gmr422
Plant Genetics   Research Article

Mutation in intron 5 of GTP cyclohydrolase 1 gene causes dopa-responsive dystonia (Segawa syndrome) in a Brazilian family

Authors: C.P. Souza, E.R. Valadares, A.L.C. Trindade, V.L. Rocha, L.R. Oliveira and A.L.B. Godard

Dopa-responsive dystonia (DRD), also known as Segawa syndrome or hereditary progressive dystonia with diurnal fluctuation, is clinically characterized by the occurrence of simultaneous or late Parkinsonism and by an excellent response to treatment with low doses of L-dopa. Diagnosis of DRD is essentially clinical. It is based .. Read More»

Genet. Mol. Res. 7(3):
vol7-3gmr467
DOI:
10.4238/vol7-3gmr467
Plant Genetics   Research Article

Evolution of the SEC1 gene in New World monkey lineages (Primates, Platyrrhini)

Authors: B.N. Borges, T.S. Paiva and M.L. Harada

The structure and evolution of the SEC1 gene were examined for the first time in New World primates of the genera Alouatta, Aotus, Ateles, Brachyteles, Callicebus, Callithrix, Cebus, Chiropotes, Lagothrix, Leontopithecus, Pithecia, Saguinus, and Saimiri. This gene has a high CG content (63.8%) and an estimated heterogeneous si.. Read More»

Genet. Mol. Res. 7(3):
vol7-3gmr452
DOI:
10.4238/vol7-3gmr452
Plant Genetics   Research Article

Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species

Authors: E.M. Gatphoh, S.K. Sharma, K. Rajkumari and S. Rama Rao

The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, bo.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1083
DOI:
10.4238/vol10-3gmr1083
Plant Genetics   Research Article

Isolation and characterization of microsatellite loci from an endangered tree species, Toona ciliata var. pubescens

Authors: J. Liu Z.-X. Sun Y.-T. Chen J.-M. Jiang

Toona ciliata var. pubescens is considered an endangered tree species native to China. In order to help develop a conservation program for this species, we evaluated its genetic diversity and population genetics. We isolated microsatellite DNA loci using streptavidin beads. A genomic library, enriched with microsatellites, was constructed and screened by .. Read More»

Genet. Mol. Res. 11(4):
2012.September.19.4
DOI:
10.4238/2012.September.19.4
Plant Genetics   Research Article

Identification and expression analysis of multiple FRO gene copies in Medicago truncatula

Authors: Ma. del C. Orozco-Mosqueda G. Santoyo R. Farias-Rodriguez L. Mac�­as-Rodr�­guez E. Valencia-Cantero

Iron (Fe) is an essential element for plant growth. Commonly, this element is found in an oxidized form in soil, which is poorly available for plants. Therefore, plants have evolved ferric-chelate reductase enzymes (FRO) to reduce iron into a more soluble ferrous form. Fe scarcity in plants induce the FRO enzyme activity. Although the legume Medicago trun.. Read More»

Genet. Mol. Res. 11(4):
2012.October.9.7
DOI:
10.4238/2012.October.9.7
Plant Genetics   Research Article

Molecular cloning and characterization of two novel NAC genes from Mikania micrantha (Asteraceae)

Authors: D.M. Li J.H. Wang S.L. Peng G.F. Zhu F.B. LÃ?Â?

NAC proteins, which are plant-specific transcription factors, have been identified to play important roles in plant response to stresses and in plant development. The full-length cDNAs that encode 2 putative NAC proteins, designated as MmATAF1 and MmNAP, respectively, were cloned from Mikania micrantha by rapid amplification of cDNA ends. The full-length cDN.. Read More»

Genet. Mol. Res. 11(4):
2012.September.19.3
DOI:
10.4238/2012.September.19.3
Plant Genetics   Research Article

Chloroplast DNA polymorphism and evolutional relationships between Asian cultivated rice (Oryza sativa) and its wild relatives (O. rufipogon)

Authors: W.J. Li B. Zhang G.W. Huang G.P. Kang M.Z. Liang L.B. Chen

We analyzed chloroplast DNA (cpDNA) polymorphism and phylogenic relationships between 6 typical indica rice, 4 japonica rice, 8 javanica rice, and 12 Asian common wild rice (Oryza rufipogon) strains collected from different latitudes in China by comparing polymorphism at 9 highly variable regions. One hundred and forty-four polymorphic bases were detected.. Read More»

Genet. Mol. Res. 11(4):
2012.October.9.8
DOI:
10.4238/2012.October.9.8
Plant Genetics   Research Article

Essential amino acid usage and evolutionary nutrigenomics of eukaryotes - insights into the differential usage of amino acids in protein domains and extra-domains

Authors: L. Santana-Santos, F. Prosdocimi and J.M. Ortega

Nutrigenomics studies the effects of nutrients on the genome, transcriptome and proteome of organisms, and here an evolutionary standpoint on this new discipline is presented. It is well known that metazoan organisms are unable to synthesize all amino acids necessary to produce their proteins and that these essential amino aci.. Read More»

Genet. Mol. Res. 7(3):
vol7-3x-meeting002
DOI:
10.4238/vol7-3x-meeting002
Plant Genetics   Research Article

In silico comparative analysis of tylenchid nematode pectate lyases

Authors: R.M. Moraes Filho and L.S.S. Martins

Phytoparasitic nematodes can infect a wide range of crop plants, and cause billions of dollars of agricultural losses each year. These parasites represent the largest source of biotic stress experienced by plants. The order Tylenchida comprises the most important parasitic nematodes, particularly the root-knot and cyst nematodes. These parasitic organisms.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038402
DOI:
10.4238/gmr.15038402