Animal Genetics
Effects of Maytenus ilicifolia on reproduction and embryo-fetal development in Wistar rats
Authors: A.L. Cunha-Laura, S.A. Auharek, R.J. Oliveira, J.M. Siqueira, M.C. Vieira, V.S. Leite and L.C. Portugal
Maytenus ilicifolia (Celastraceae), popularly known as espinheira-santa, is a native plant from the Atlantic forest and is commonly used in popular medicine to treat inflammation and as an abortifacient. To evaluate the effects of M. ilicifolia on pregnant rats during the organogenic period (T1) or throughout the gestational p.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.16
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.16
Molecular characterization of the pseudorabies virus UL2 gene
Authors: M.S. Cai1,2, B.Y. Wang2, W. Cui1, Z.Y. Zhao1, J.H. Chen2, X.M. Wen1, Z. Li1 and M.L. Li1
A 948-bp sequence of the UL2 gene was amplified from the pseudorabies virus (PRV) Becker strain genome using polymerase chain reaction, and the gene identity was confirmed through further cloning and sequencing. Bioinformatic analysis indicated that the PRV UL2 gene encodes a putative polypeptide with 315-amino acid residues. Its encoding protein, designa.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.1
- DOI:
- 10.4238/2013.October.7.1
Effect of the Jianpi Bushen Prescription on expressions of the Wnt3a and Cyclin D1 genes in radiation-damaged mice
Authors: D.C. He*, J.J. Xiao*, Y. Zhang, H. Lin, X.J. Ding and Y. Tu
The effects of the traditional Chinese drug Jianpi Bushen Prescription (JBP) were investigated on expressions of Wnt3a and Cyclin D1 genes in radiation-damaged mice. The radiation damage model was induced in Kumming mice by single total body irradiation treatment for 9 days. Mice were divided into the radiation group, low-dose (100%) JBP group, high-dose .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.3.1
- DOI:
- 10.4238/2013.October.3.1
Screening of the residual normal ovarian tissue adjacent to orthotopic epithelial ovarian carcinomas in nude mice
Authors: G.H. Zhu, S.T. Wang, M.Z. Yao, J.H. Cai, C.Y. Chen, Z.X. Yang, L. Hong and S.Y. Yang
The objective of this study was to explore the feasibility and methods of screening the residual normal ovarian tissue adjacent to orthotopic ovarian carcinomas in nude mice. Human epithelial ovarian cancer cells (OVCAR3) were subcutaneously implanted for a tumor source and ovarian orthotopic transplantation. The cancer tissue.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.6
- DOI:
- 10.4238/2014.April.16.6
Correlation of growth-related traits and their effects on body weight of the mud crab (Scylla paramamosain)
Authors: H.Y. Ma, C.Y. Ma, L.B. Ma, Z. Xu, N.N. Feng and Z.G. Qiao
In this study, we analyzed the correlation of 20 growth-related traits and their effects on body weight of Scylla paramamosain. The correlation coefficients in all trait pairs were significantly high, ranging from 0.551 to 0.999. Among 19 X-Y pairs, the correlation coefficient between traits X1 and Y was the highest, whereas that between X13 and Y was the.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.1.3
- DOI:
- 10.4238/2013.October.1.3
Expression analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle
Authors: Y. Liu, H. Wang, G. Cheng B. Jiang and L. Zan
The Hedgehog (Hh) signaling pathway regulates the differentiation of many kinds of cells and plays a critical role in many embryonic and postnatal developmental processes. Gli1 and Gli2 are two transcription factors of the Hh signaling pathway. In this study, we used quantitative real-time polymerase chain reaction to detect the relative expression of Gli.. Read More»
- Genet. Mol. Res. 13(4):
Novel DNA extraction assay for molecular identification of Aedes spp eggs
Authors: M.T.S. Freitas, P.P. Gomes-J�ºnior, M.V.A. Batista, T.C. Leal-Balbino, A.L. Araujo and V.Q. Balbino
Aedes aegypti and A. albopictus represent the two most important species of mosquitoes in relation to dengue virus transmission both in the Americas and Asia. However, the study of theses species generally requires the establishment of a colony for the larvae to hatch, or waiting for the adult development to perform its taxonomic classification, which is .. Read More»
- Genet. Mol. Res. 13(4):
Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle
Authors: D.B.S. Silva, B.A. Crispim, L.E. Silva, J.A. Oliveira, F. Siqueira, L.O. Seno and A.B. Grisolia
The aim of the study was to detect polymorphisms in the leptin gene and to determine the association of these polymorphisms with growth and carcass traits in Nellore cattle. The single nucleotide polymorphisms (SNPs) -1457 (AJ571671:g.-1457A>G) and A59V (AF536174.1:g. 321C>T), as well as the microsatellite BM1500 (3.9 kb.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.9
- DOI:
- 10.4238/2014.April.16.9
Comparative study between Ephedra sinica Stapf and Fructus Schisandrae Chinensis on ET-1 and 6-keto-prostaglandin F1�± in rats with idiopathic pulmonary fibrosis
Authors: H.Q. Zhai, J.R. Hhang, M.C. Gao, Y. Liu, S.F. Zhang, X.H. Wang, F.Y. Meng and Y.P. Wang
The aim of this study was to evaluate differences in the effects of Ephedra sinica Stapf and Fructus Schisandrae Chinensis on angiogenesis in the treatment of bleomycin-induced rat idiopathic pulmonary fibrosis. The rat models were created using bleomycin. The animals were divided into six groups: model, control, Ephedra alone.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.13.3
- DOI:
- http://dx.doi.org/10.4238/2014.May.13.3
Isolation and characterization of polymorphic microsatellite loci in the swimming crab Portunus trituberculatus (Portunidae) L.P.
Authors: L.P. Ren, Y. Qin, X.C. Li, Y.N. Sun, R.X. Wang
The Swimming crab Portunus trituberculatus (Portunidae) is an important economically food species. To provide molecular markers for P. trituberculatus, we isolated and characterized polymorphic microsatellite markers. We developed a 5'-anchored genomic library of P. trituberculatus DNA, and derived 45 positive clones. We desig.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.19
- DOI:
- 10.4238/2013.November.22.19