Animal Genetics
Evaluation of perfused bovine udder for gene expression studies in dairy cows
Authors: I.S.B. Pinto, I. Fonseca, H.M. Brand�£o, J.C. Gern3, A.S. Guimar�£es, W.A. Carvalho, M.A.V.P. Brito, L.F. Viccini, M.F. Martins
Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfuse.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019637
- DOI:
- 10.4238/gmr16019637
Establishment of reference sequences of hepatitis B virus genotype C subgenotypes
Authors: H.L. Zhu, C.T. Wang, J.B. Xia, X. Li and Z.H. Zhang
Hepatitis B virus genotype C (HBV/C) has the largest number of subgenotypes (C1-C16) that vary with geography and isolates. HBV/C prevails in Southeast Asia (C1, C5-C16), East Asia (C2), Oceania (C3), and Australia (C4). Suitable reference strains for different subgenotypes could greatly facilitate research into HBV/C, but unfortunately they are scarce. W.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo
Authors: H.B. Chen, X.C. Zhang, Y.F. Cheng, A. Abdelnasir, S. Tang,N. Kemper, J. Hartung and E.D. Bao
To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro. After exposure to heat s.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.9
- DOI:
- 10.4238/2015.March.20.9
Copy number and integration sites in growth hormone transgenic goats
Authors: Q. Zhang, J. Lin, Q.H. Yu, W.W. Hu and Q. Yang
Transgenic goats have been utilized for years to produce valuable protein. However, when transgenic goats are produced by random integration of inserted genes into cells, the copy number and integration sites of these genes in the goat genome are typically indefinite. Most polymerase chain reaction (PCR)-based methods that have been utilized to determine .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.10
- DOI:
- 10.4238/2015.March.20.10
Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.
Authors: S.H. Wang, Y. Li, Z.Q. Li, L. Chang and L. Li
Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified p.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.11
- DOI:
- 10.4238/2015.March.20.11
14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy
Authors: D.M. Xue, X.Q. Guo, R. Chen, Z.P. Niu and C.S. Xu
14-3-3 Proteins are a ubiquitous family of molecules that participate in protein kinase signaling pathways in all eukaryotic cells. Functioning as phosphoserine/phosphothreonine-binding modules, 14-3-3 proteins participate in the phosphorylation-dependent protein-protein interactions that control progression through the cell cycle, initiation and.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.12
- DOI:
- 10.4238/2015.March.20.12
Identification of SNPs located on BTA 6 and BTA 20 significantly associated with bovine respiratory disease in crossbred cattle
Authors: S.L. Miller, S. Mizell, R. Walker, T. Page and M.D. Garcia
The objective of the present study was to evaluate single nucleotide polymorphisms (SNPs) located in two quantitative trait locus (QTL) regions (BTA 6 and BTA 20) that are associated with bovine respiratory disease (BRD). A population of 323 crossbred steers sired by five purebred sire breeds during 2010-2013 (Angus, Braford, .. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048861
- DOI:
- 10.4238/gmr.15048861
High-resolution melting curve analysis of the ADSL and LPL genes and their correlation with meat quality and blood parameters in chickens
Authors: X.D. Zhang, Q.H. Li, L.F. Lou, J. Liu, X.H. Chen, C.X. Zhang and H.H. Wang
Adenylosuccinate lyase (ADSL) and lipoprotein lipase (LPL) are key enzymes in the metabolism of inosine monophosphate (IMP) and fat mass, which are important factors in meat quality evaluation. In this study, we selected 50 hens from the ISA B-line layers and Guangxi Yellow chickens, slaughtered the chickens at 120 days old, and analyzed polymorphisms in .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.13
- DOI:
- 10.4238/2015.March.20.13
Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes
Authors: H. Qiao, Y.W. Xiong, S.F. Jiang, H.T. Fu, S.M. Sun, S.B. Jin, Y.S. Gong and W.Y. Zhang
This study utilized high-throughput RNA sequencing technology to identify reproduction- and development-related genes of Macrobrachium nipponense by analyzing gene expression profiles of testis and ovary. More than 20 million 1 x 51-bp reads were obtained by Illumina sequencing, generating more than 7.7 and 11.7 million clean reads in the testis and ovary.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.14
- DOI:
- 10.4238/2015.March.20.14
Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis
Authors: E.C.B. Silva, M.A. Pelinca, A.C. Acosta, D.M.F. Silva, M.A. Gomes Filho and M.M.P. Guerra
Successful DNA extraction is indispensable for molecular methods based on polymerase chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this study was to evaluate three methods to extract DNA from goat sperm for use in PCR. Eight goat semen pools were used for DNA extraction by using the DNea.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.21
- DOI:
- 10.4238/2014.August.7.21