Animal Genetics
Cloning and expression analysis of a heat shock protein 90 �² isoform gene from the gills of Wuchang bream (Megalobrama amblycephala Yih) subjected to nitrite stress
Authors: S.M. Sun, J. Zhu, X.P. Ge, C.F. Zhang, L.H. Miao and X.J. Jiang
Heat shock protein 90 (HSP90), a highly conserved and multi-functional molecular chaperone, plays an essential role in cellular metabolism and stress response. In this study, HSP90 cDNA named MaHSP90 was cloned from Wuchang bream (Megalobrama amblycephala) gills by using rapid amplification of cDNA ends. The full-length MaHSP90 cDNA is 2674 bp and consist.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.14
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.14
Effects of sex and age on chicken TBC1D1 gene mRNA expression
Authors: Y.D. Peng, H.Y. Xu, F. Ye, X. Lan, X. Peng, A. Rustempa�¡i��, H.D. Yin, X.L. Zhao, Y.P. Liu, Q. Zhu and Y. Wang
The objective of this study was to investigate the effects of sex and slaughter age of chickens on fatty acid composition and TBC1D1 gene expression in 4 different tissues: breast muscle, thigh muscle, abdominal fat, and subcutaneous fat. Sixty Erlang mountainous chickens (hybrid SD02 x SD03) were raised under the same conditions and slaughtered at 8, 10,.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.16
- DOI:
- 10.4238/2015.July.13.16
Colony membership is reflected by variations in cuticular hydrocarbon profile in a Neotropical paper wasp, Polistes satan (Hymenoptera, Vespidae)
Authors: Tannure-Nascimento IC, Nascimento FS, Turatti IC, Lopes NP, Trigo JRand Zucchi R
Nestmate recognition is one the most important features in social insect colonies. Although epicuticular lipids or cuticular hydrocarbons have both structural and defensive functions in insects, they also seem to be involved in several aspects of communication in wasps, bees and ants. We analyzed and described for the first ti.. Read More»
- Genet. Mol. Res. 6(2):
Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design
Authors: B. Zeng, X. Huang, L.K. Huang, J. Zhang, H.D. Yan, D. Luo, H. Liang and Y. Yuan
The effects of 5 factors (template DNA, Mg2+, dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymorphism (SCoT)-PCR system of Dactylis glomerata L., using an orthogonal design L16 (45). A suitable SCoT-PCR system for D. glomerata was established; the 20 μL reaction v.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.15
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.15
Different magnitude of resistance to non-depolarizing muscle relaxants in dexamethasone-treated rat diaphragm associated with altered acetylcholine receptor expression
Authors: D. Chen, M.R. Yang, L.N. Huang, Y.W. Qiu and S.T. Li
The aim of this study was to investigate the influence of chronic dexamethasone (Dex) administration on rat diaphragm sensitivity to non-depolarizing muscle relaxants (NDMRs) and muscular nicotinic acetylcholine receptor (nAChR) expression, which may help direct future administration of NDMRs. Adult male Sprague-Dawley rats we.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.4
- DOI:
- 10.4238/2014.August.7.4
RNA interference of leptin receptor in chicken adipocytes
Authors: A.X. Huang, J.J. Li, Y. Tian, J.D. Shen, Z.R. Tao, G.Q. Li, L.Z. Lu, Y. Fu and T.X. Wu
In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P peroxisome proliferator-activated receptor γ, fatty acid synthase, .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.5
- DOI:
- 10.4238/2014.August.7.5
Genetic diversity of microsatellite loci in Leopardus pardalis, Leopardus wiedii and Leopardus tigrinus
Authors: Grisolia AB, Moreno VR, Campagnari F, Milazzotto MP, Garcia JF, Adania CH and Souza EB
The microsatellite loci FCA045, FCA077, FCA008, and FCA096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive Leopardus sp. The PCRamplified products of microsatellite loci were characterized in ABI Prism 310 Genetic Analyzer. Allele numbers, heterozygosity, polymorphism inf.. Read More»
- Genet. Mol. Res. 6(2):
Performance of human immunostimulating agents in the improvement of fish cytogenetic preparations
Authors: W.F. Molina, D.E.O. Alves, W.C. Ara�ºjo, P.A. Martinez, M.F.M. Silva and G.W.W.F. Costa
Inoculation with bacterial or fungal antigens that stimulate cell proliferation has been widely used to obtain metaphases for cytogenetic studies of fish. We evaluated the potential of new pharmaceutical compounds as mitogenic agents in fish, testing the efficacy of Aminovac® (mixed antigens and epsilon-acetamidocaproic ac.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr840
- DOI:
- 10.4238/vol9-3gmr840
Changes in cyclic GMP level and phosphodiesterase activity during follicular development in the rat ovary
Authors: C.Q. Fu, F.X. Shi, Z.H. Zhang, J.R. Li, X.H. Huang and Z.C. Wang
Guanosine 3',5'-cyclic monophosphate (cGMP), as a second messenger, plays potential roles in ovarian functions. To elucidate the role of phosphodiesterase (PDE) in cGMP signaling during ovarian follicular development, the present study was conducted to investigate ovarian cGMP level and cGMP-PDE activity by radioimmunoassay (R.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.7
- DOI:
- 10.4238/2014.August.7.7
Variation and genetic structure of Tunisian Festuca arundinacea populations based on inter-simple sequence repeat pattern
Authors: N. Chtourou-Ghorbel, H. Elazreg, S. Ghariani, N. Ben Mheni, M. Sekmani, M. Chakroun and N. Trifi-Farah
Tunisian tall fescue (Festuca arundinacea Schreb.) is an important grass for forages or soil conservation, particularly in marginal sites. Inter-simple sequence repeats were used to estimate genetic diversity within and among 8 natural populations and 1 cultivar from Northern Tunisia. A total of 181 polymorphic inter-simple sequence repeat markers were ge.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.17
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.17