Animal Genetics
DNA extraction from hair shafts of wild Brazilian felids and canids
Authors: C.C. Alberts, J.T. Ribeiro-Paes, G. Aranda-Selverio, J.R. Cursino-Santos, V.R. Moreno-Cotulio, A.L.D. Oliveira, B.F.M.M. Porchia, W.F. Santos and E.B. Souza
Wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. Due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. DNA found in hair shafts can be used in molecular studies that can unravel, for ins.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr1027
- DOI:
- 10.4238/vol9-4gmr1027
Gene expression, serum amino acid levels, and growth performance of pigs fed dietary leucine and lysine at different ratios
Authors: H. GarcÃ?Âa, A. Morales, A. Araiza, J.K. Htoo and M. Cervantes
We examined 96 pigs (28.1 ± 0.83 kg) to analyze the effect of Leu:Lys ratios on expression of the cationic amino acid transporters b0,+ and CAT-1 in the jejunum and liver as well as myosin expression in 2 muscles to estimate the optimum standardized ileal digestible (SID) Leu:Lys ratio for growth rate and efficiency. A wheat-and wheat bran-based di.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.6
- DOI:
- 10.4238/2015.March.6.6
Cloning, partial sequence, and single-nucleotide polymorphism of the ryanodine receptor gene of the Pacific white shrimp Litopenaeus vannamei (Penaeidae)
Authors: X.H. Chen, D.G. Zeng and N. Ma
Ryanodine receptor/calcium release channel is a large protein that plays an essential role in muscle contraction; mutations in the ryanodine receptor gene affect sensitivity to stress. As a first step towards investigating the relationship between the ryanodine receptor and shrimp cramped muscle syndrome, we cloned, partially .. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr976
- DOI:
- 10.4238/vol9-4gmr976
Association of CAPN1 316, CAPN1 4751 and TG5 markers with bovine meat quality traits in Mexico
Authors: C.A. Bonilla, M.S. Rubio, A.M. Sifuentes, G.M. Parra-Bracamonte, V.W. Arellano, M.R.D. M�©ndez, J.M. Berruecos and R. Ortiz
We examined allele and genotype frequencies for the molecular markers CAPN1 316, CAPN1 4751 and TG5, and determined whether they are associated with beef quality traits in Mexican cattle. One hundred and twenty-four longissimus dorsi muscle samples were collected from cattle from north, central and southern Mexico. CAPN1 316 a.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr959
- DOI:
- 10.4238/vol9-4gmr959
Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals
Authors: I.A. Arif, H.A. Khan, M. Al Sadoon and M. Shobrak
In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard dou.. Read More»
- Genet. Mol. Res. 10(4):
Random regression models using different functions to model test-day milk yield of Brazilian Holstein cows
Authors: A.B. Bignardi, L. El Faro, R.A.A. Torres J�ºnior, V.L. Cardoso, P.F. Machado and L.G. Albuquerque
We analyzed 152,145 test-day records from 7317 first lactations of Holstein cows recorded from 1995 to 2003. Our objective was to model variations in test-day milk yield during the first lactation of Holstein cows by random regression model (RRM), using various functions in order to obtain adequate and parsimonious models for .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.31.4
- DOI:
- 10.4238/2011.October.31.4
Isolation and characterization of polymorphic microsatellite loci in the hard-shelled mussel, Mytilus coruscus (Mytilidae)
Authors: T.-J. Xu, Y.-N. Sun, Y.-T. Yuan, Z. Liao and R.-X. Wang
Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to sev.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr856
- DOI:
- 10.4238/vol9-3gmr856
Correlation between liver cancer occurrence and gene expression profiles in rat liver tissue
Authors: C.S. Xu, G.P. Wang, L.X. Zhang, C.F. Chang, J. Zhi and Y.P. Hao
Liver cancer (LC) is generally characterized by malignant cell proliferation and growth; it normally develops in stages that progress from non-specific injury of the liver to liver fibrosis, liver cirrhosis, dysplasia nodules, and liver carcinoma. We used a rat model of diethylnitrosamine (DENA)-induced LC; a Rat Genome 230 2... Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.14.3
- DOI:
- 10.4238/2011.December.14.3
Precision of distances and ordering of microsatellite markers in consensus linkage maps of chromosomes 1, 3 and 4 from two reciprocal chicken populations using bootstrap sampling
Authors: M.F. Ros�¡rio, G.R.A. Margarido, C. Boschiero, A.S.A.M.T. Moura, M.C. Ledur, L.L. Coutinho and A.A.F. Garcia
Some factors complicate comparisons between linkage maps from different studies. This problem can be resolved if measures of precision, such as confidence intervals and frequency distributions, are associated with markers. We examined the precision of distances and ordering of microsatellite markers in the consensus linkage ma.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr842
- DOI:
- 10.4238/vol9-3gmr842
Genetically modified animals for use in research and biotechnology
Authors: L.M. Chaible, M.A. Corat, E. Abdelhay and M.L.Z. Dagli
Transgenic animals are used extensively in the study of in vivo gene function, as models for human diseases and in the production of biopharmaceuticals. The technology behind obtaining these animals involves molecular biology techniques, cell culture and embryo manipulation; the mouse is the species most widely used as an expe.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr867
- DOI:
- 10.4238/vol9-3gmr867