Animal Genetics
Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge
Authors: Q.Z. Yang, Z.J. Yang, Y. Zhang, X.L. Li and W. Zhang
A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.1
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.1
Differential expression of L-FABP and L-BABP between fat and lean chickens
Authors: Q. Zhang1,2, H. Shi1,2, W. Liu1,2, Y. Wang1,2, Q. Wang1,3 and H. Li1,2
Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. This study was designed to analyze and compare L-FABP and L-BABP expression levels between fat and lean lines in chicken liver tissue, and to determine the relationship be.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.5
- DOI:
- 10.4238/2013.October.7.5
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
Authors: L.N. Sun1,2, H.S. Yang1, M.Y. Chen3 and D.X. Xu1,2
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.7
- DOI:
- 10.4238/2013.November.7.7
Development of 1047 insertion-deletion markers for rice genetic studies and breeding
Authors: Y.X. Zeng*, Z.H. Wen*, L.Y. Ma, Z.J. Ji, X.M. Li and C.D. Yang
In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and j.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.7
- DOI:
- 10.4238/2013.October.30.7
Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)
Authors: W. Zhao1,2, L. Chen1, F. Zhang1, P. Wu1, E. Li1 and J. Qin3
We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino aci.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.6
- DOI:
- 10.4238/2013.October.30.6
Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study
Authors: L.E.S. Fedel-Miyasato, A.S.N. Formagio, S.A. Auharek, C.A.L. Kassuya, S.D. Navarro, A.L. Cunha-Laura, A.C.D. Monreal, M.C. Vieira and R.J. Oliveira
It is estimated that 60% of anticancer drugs are derived directly or indirectly from medicinal plants. Schinus terebinthifolius Raddi (Anacardiaceae) is traditionally used in Brazilian medicine to treat inflammation, ulcers, and tumors. Because of the need to identify new antimutagenic agents and to determine their mechanism o.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.2
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.2
Expression of calpastatin and myostatin genes associated with lamb meat quality
Authors: A. Bagatoli1, E. Gasparino1, M.A.M. Soares2, R.M. Amaral1,F.A.F. Macedo1, D.M. Voltolini1 and A.P. Del Vesco1
Calpastatin (CAST) is an endogenous calpain inhibitor and its main function is to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration. The myostatin gene (GDF-8) acts as a negative regulator of skeletal muscle growth. The expression of these two genes, as well as their interaction, affects the quality of the meat.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.3
- DOI:
- 10.4238/2013.December.4.3
Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds
Authors: Y.X. Liu, G.Q. Shi, H.X. Wang, P.C. Wan, H. Tang, H. Yang and F. Guan
High glycine-tyrosine proteins (HGTPs), also known as keratin-associated proteins (KAPs), play a key role in the major structures and mechanical properties of wool fiber. Sheep HGTPs consist of three multigene families: KAP6, KAP7, and KAP8 genes. Polymorphisms of these three genes have been proposed to have important effects .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.5
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.5
Cross-amplification of nonspecific micro-satellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer
Authors: J.C. MarÃ?Ân, P. Orozco-terWengel, K. Romero, J.P. VÃ?¡squez, V. Varas and J.A. Vianna
Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully i.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.4
- DOI:
- 10.4238/2014.April.25.4
Isolation and characterization of novel microsatellite loci in Nibea albiflora
Authors: D.D. Xu, B. Lou, S.L. Li, Y.R. Zhang, J. Xin and W. Zhan
Eleven novel microsatellite loci were isolated from a (CA)10-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected heterozy.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.1
- DOI:
- 10.4238/2013.December.4.1