Animal Genetics
Utility of hair shafts from study skins for mitochondrial DNA analysis
Authors: N. Kurihara
The condition of mtDNA in hair shafts preserved in a museum was examined using 30 study skins of masked palm civets, Paguma larvata (Viverridae), collected between 1924 and 2011. Comparisons of extracts from fresh and burnt alum-fixed hair shafts showed that burnt alum, which is commonly used in taxidermy, had no harmful effect on the amount of total DNA .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.1
- DOI:
- 10.4238/2013.November.11.1
Molecular genetic diversity and maternal origin of Chinese black-bone chicken breeds
Authors: W.Q. Zhu, H.F. Li, J.Y. Wang, J.T. Shu, C.H. Zhu, W.T. Song, C. Song, G.G. Ji and H.X. Liu
Chinese black-bone chickens are valued for the medicinal properties of their meat in traditional Chinese medicine. We investigated the genetic diversity and systematic evolution of Chinese black-bone chicken breeds. We sequenced the DNA of 520 bp of the mitochondrial cyt b gene of nine Chinese black-bone chicken breeds, includ.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.5
- DOI:
- 10.4238/2014.April.29.5
Experimental evaluation of the reproductive quality of Africanized queen bees (Apis mellifera) on the basis of body weight at emergence
Authors: D.A. De Souza1, M.A.F. Bezzera-Laure1, T.M. Francoy2 and L.S. Gon�§alves3,4
There has been much speculation about which phenotypic traits serve as reliable indicators of productivity in queen honeybees (Apis mellifera). To investigate the predictive value of queen body weight on colony development and quality, we compared colonies in which queens weighed less than 180 mg to those in which queens weighed more than 200 mg. Both gro.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.13
- DOI:
- 10.4238/2013.November.7.13
Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk
Authors: T. Usman, Y. Yu, C. Liu, Z. Fan and Y. Wang
Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three methods, Modified Nucleospin Blood Ki.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.10
- DOI:
- 10.4238/2014.April.29.10
Adjacent SNPs in the transcriptional regulatory region of the FADS2 gene associated with fatty acid and growth traits in chickens
Authors: S.K. Zhu, Y.D. Tian, S. Zhang, Q.X. Chen, Q.Y.Wang,R.L. Han and X.T. Kang
Delta-6 fatty acid desaturases are rate-limiting desaturases involved in metabolic processes of fatty acids, and they are encoded by the FADS2 gene. In the current study, an F2 resource population of Gushi chickens crossed with Anak broilers was used to investigate the genetic effects of the chicken FADS2. Two adjacent single .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.11
- DOI:
- 10.4238/2014.April.29.11
The first assess of the haplotypes from COI gene sequences in species of spittlebugs (Cicadomorpha: Hemiptera) and aquatic true bugs (Gerromorpha and Nepomorpha: Hemiptera) in Brazil
Authors: M.M.U. Castanhole1, S.R.C. Marchesin2, L.L.V. Pereira1, F.F.F. Moreira3, J.F. Barbosa3, J.R. Val�©rio4 and M.M. Itoyama1
We made the first analysis of the COI gene sequences of 22 species of spittlebugs and aquatic true bugs sampled in São Paulo State (Brazil) and used this information to determine the variability within these groups. Considering each codon position, we observed that the third base was the most variable, and the first base was the most conserved. Amo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.12
- DOI:
- 10.4238/2013.November.7.12
Effect of secondary lymphoid tissue chemokine suppression on experimental ulcerative colitis in mice
Authors: H. Zhang, X. Zhang, X. Ding, W. Cao, L. Qu and G. Zhou
The secondary lymphoid tissue chemokine (CCL21) is closely associated with lymphoid homing and anti-tumor immune responses. CCL21 also has a chemotactic effect on intestinal lymphocytes. This study mainly focused on CCL21 expression in experimental ulcerative colitis and on the effects of CCL21 suppression on this disease in m.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.12
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.12
Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor
Authors: X.S. Wang, H. Ao, L.W. Zhai, L.J. Bai, W.Y. He, C.D. Wang, Y. Yu
The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFNα were significantly upregulated by PolyI:C, compared to the untreated PK15 cells, whic.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.13
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.13
Genetic diversity of local Yunnan chicken breeds and their relationships with Red Junglefowl
Authors: J.L. Huo, G.S. Wu, T. Chen, H.L. Huo, F. Yuan, L.X. Liu, C.R. Ge and Y.W. Miao
Yunnan is situated in the Southwest China and encompasses regions having high biodiversity, including habitats for several ancestral species of domestic animals such as chicken. Domestic chickens in Yunnan were kept by peoples of varied ethnic and economic backgrounds living in highly varied geographic environments. To identif.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.16
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.16
Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer
Authors: J.C. MarĂ?Ân, P. Orozco-terWengel, K. Romero, J.P. VĂ?¡squez, V. Varas and J.A. Vianna
Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully i.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.4
- DOI:
- 10.4238/2014.April.25.4