Animal Genetics
Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix
Authors: M. Cristancho and C. Escobar
The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR m.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr493
- DOI:
- 10.4238/vol7-4gmr493
Utility of STR markers for the molecular diagnosis of a large Brazilian family with Charcot-Marie-Tooth disease
Authors: C.O. Possamai, F.M. Carvalho, M.F.C. Silva, E.V. Wolfgramm, M.P.N. Sartori, F.S.V. Malta, V.P. Ribeiro, V.P. Spina, K.B. Gomes, A.C.S. Ferreira and I.D. Louro
Charcot-Marie-Tooth type 1A disease (CMT1A) is most frequently caused by a tandem DNA duplication of a 1.4-Mb genomic fragment in the 17p11.2-12 chromosomal region. The disease is probably the product of a dosage effect of the peripheral myelin protein 22 gene located within the duplicated segment. We sought to study the largest reported Brazilian family .. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr500
- DOI:
- 10.4238/vol7-4gmr500
Differential expression of the MHM region and of sex-determining-related genes during gonadal development in chicken embryos
Authors: L.C. Caetano, F.G.O. Gennaro, K. Coelho, F.M Ara�ºjo, R.A. Vila,A. Ara�ºjo, A. de Melo Bernardo, C.R. Marcondes, S.M. Chuva de Sousa Lopes, and E.S. Ramos
The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.13.2
- DOI:
- 10.4238/2014.February.13.2
Differential expression of interleukin-12 p35 and p40 subunits in response to Aeromonas hydrophila and Aquareovirus infection in grass carp, Ctenopharyngodon idella
Authors: N.P. Pandit,Y.B. Shen,X.Y. Xu,H.Y. Yu,W.J. Wang,R.Q. Wang,Y.F. Xuan,J.L. Li
The grass carp (Ctenopharyngodon idella) aquaculture industry in Asia is prone to bacterial and viral hemorrhagic diseases. Effective adjuvants for vaccine formulation are the need of the hour for control of these diseases and long-term sustainability of grass carp farming. In this study, the involvement of interleukin-12 (IL-12) from grass carp (gcILâÂ?Â?1.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.20
- DOI:
- 10.4238/2015.February.6.20
A novel COL1A1 gene-splicing mutation (c.1875+1G>C) in a Brazilian patient with osteogenesis imperfecta
Authors: C. Barbirato M.G. Almeida M. Milanez V. Sipolatti M.R.G.O. Rebou�§as A.N. Akel Jr. V.R.R. Nunes A.M.S. Perrone M. Zatz I.D. Louro F. Paula
Osteogenesis imperfecta is a heterogeneous genetic disorder characterized by bone fragility and deformity, recurrent fractures, blue sclera, short stature, and dentinogenesis imperfecta. Most cases are caused by mutations in COL1A1 and COL1A2 genes. We present a novel splicing mutation in the COL1A1 gene (c.1875+1G>C) in a .. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr563
- DOI:
- 10.4238/vol8-1gmr563
Analysis of POU1F1 gene DdeI polymorphism in Chinese goats
Authors: M.J. Li1,2, C.M. Zhang2, X.Y. Lan1, X.T. Fang3, C.Z. Lei1 and H. Chen1
As a member of the POU-domain family, the POU1F1 is a positive regulator for growth hormone, prolactin and thyroid-stimulating hormone b, by binding to target DNA promoters as a dimer in mammals. This study described the polymorphisms at the goat POU1F1-DdeI locus and analyzed the distribution of alleles in 15 indigenous Chinese goat breeds. The PCR-RFLP .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017747
- DOI:
- 10.4238/gmr.15017747
Cytogenetic analysis and description of the sexual chromosome determination system ZZ/ZW of species of the fish genus Serrapinnus (Characidae, Cheirodontinae)
Authors: Santi-Rampazzo AP, Nishiyama PB, Ferreira PEB and Martins-Santos IC
Four populations of Serrapinnus notomelas and one population of Serrapinnus sp.1, both belonging to the subfamily Cheirodontinae, were analyzed by Giemsa and silver nitrate impregnation techniques. We found 2n = 52 chromosomes for all populations, with interspecific differences in the karyotype formula; S. notomelas showed 16m.. Read More»
- Genet. Mol. Res. 6(3):
Environmental and genetic factors affecting the weaning-estrus interval in sows
Authors: C.D.S. Leite, J.F. Lui, L.G. Albuquerque and D.N.M. Alves
We examined the effects of environmental and genetic factors on the weaning-to-estrus interval (WEI) in sows. In order to perform the analyses of the environmental factors, 8104 observations of the 1st to the 6th WEI were carried out, while 6548 observations of the 1st to the 3rd WEI were carried out for the analyses of geneti.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.4.2
- DOI:
- 10.4238/2011.November.4.2
Comparative study of the cuticular hydrocarbon in queens, workers and males of Ectatomma vizottoi (Hymenoptera, Formicidae) by Fourier transform-infrared photoacoustic spectroscopy
Authors: Antonialli Junior WF, Lima SM, Andrade LHC and Suarez YR
Fourier transform-infrared photoacoustic spectroscopy was applied for the first time, to our knowledge, to distinguish different castes of an ant species. The method was applied directly to the abdomen of queens, workers and males of Ectatomma vizottoi ants, without any special sample preparation. The absorption bands of secon.. Read More»
- Genet. Mol. Res. 6(3):
Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi
Authors: N. Ally X.L. Zou B.C. Jiang L. Qin L. Zhai P. Xiao H.L. Liu
Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonuc.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.28.1
- DOI:
- 10.4238/2012.November.28.1