Animal Genetics
Phylogenetic analysis of widely cultivated Ganoderma in China based on the mitochondrial V4-V6 region of SSU rDNA
Authors: X.W. Zhou, K.Q. Su and Y.M. Zhang
Ganoderma mushroom is one of the most prescribed traditional medicines and has been used for centuries, particularly in China, Japan, Korea, and other Asian countries. In this study, different strains of Ganoderma spp and the genetic relationships of the closely related strains were identified and investigated based on the V4-V6 region of mitochondrial sm.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.2.12
- DOI:
- 10.4238/2015.February.2.12
Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification
Authors: Y.H. Dong1, H.H. Yao1, C.S. Sun2, D.M. Lv1, M.Q. Li1 and Z.H. Lin1
Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were de.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017285
- DOI:
- 10.4238/gmr.15017285
Genetic trends of absolute and relative heart weight in a male broiler line
Authors: Gaya LG, Costa AMMA, Ferraz JBS, Rezende FM, Mattos EC, Eler JP, Michelan Filho T, Mourao GB and Figueiredo LGG
Data of chickens from a broiler-breeding program have been collected and used for determination of genetic trends of absolute and relative heart weight. The genetic trends have been estimated by regression of the genetic values of the traits over hatch-year. Genetic values of 42,912 individuals, obtained by restricted maximum .. Read More»
- Genet. Mol. Res. 6(4):
Evaluation of genetic variability in micropropagated propagules of ornamental pineapple [Ananas comosus var. bracteatus (Lindley) Coppens and Leal] using RAPD markers
Authors: M.D.M. Santos1, G.C.S. Buso2 and A.C. Torres3
The objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. The basal culture medium consisted of MS salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr489
- DOI:
- 10.4238/vol7-4gmr489
Equol promotes rat osteoblast proliferation and differentiation through activating estrogen receptor
Authors: J. Wang, J. Xu, B. Wang, F.R. Shu, K. Chen and M.T. Mi
Phytoestrogens have been suggested as alternative treatment for postmenopausal osteoporosis. Equol, a metabolite of daidzein, has been shown to inhibit bone loss in ovariectomized mice and rats. However, whether or not equol influences the formation of bone has not yet been investigated. Therefore, we investigated the effect o.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.21
- DOI:
- 10.4238/2014.July.4.21
Construction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm
Authors: Z. Song, Z.H. Li, X.Q. Lei, T.S. Xu, X.H. Zhang, Y.J. Li, G.M. Zhang, S.M. Xi, Y.B. Yang and Z.G. Wei
The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.2.16
- DOI:
- 10.4238/2015.February.2.16
Association of single nucleotide polymorphisms in the bovine leptin and leptin receptor genes with growth and ultrasound carcass traits in Nellore cattle
Authors: R.C.G. da Silva J.B.S. Ferraz F.V. Meirelles J.P. Eler J.C.C. Balieiro D.C. Cucco E.C. Mattos F.M. Rezende S.L. Silva
Given the important role of leptin in metabolism, we looked for a possible association of leptin and leptin receptor polymorphisms with carcass and growth traits in Nellore cattle. We examined associations of leptin and leptin receptor SNPs with ultrasound carcass (longissimus dorsi muscle area (ribeye area), backfat thickness and rump fat thickness and grow.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.August.17.10
- DOI:
- 10.4238/2012.August.17.10
Sequence analysis of the S1PR1 gene in river buffalo
Authors: N.B. Stafuzza1,2, B.C.M. Naressi1, M.M. Borges1 and M.E.J. Amaral-Trusty1
Recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. The S1PR1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. Here, we performed next generation sequen.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016363
- DOI:
- 10.4238/gmr.15016363
Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus
Authors: L.Lin, C.H. Li, S.N. Xu, Y. Liu and Y.Y. Xiao
We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650,.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.21.5
- DOI:
- http://dx.doi.org/10.4238/2015.September.21.5
shRNA inhibits the expression of chicken telomerase reverse transcriptase in MDCC-MSB1 cells
Authors: N. Jiang, X.B. Zheng, Z.Y. Zhao, Z.G. Qin and T.J. Liu
Here, we investigated the effects of blocking chicken telomerase reverse transcriptase (chTERT) in MDCC-MSB1 cells, using small-hairpin RNAs (shRNAs) to interfere with gene expression. shRNAs specific to chTERT mRNA were designed, cloned into DNA plasmid vectors, and transfected into MDCC-MSB1 cells. The transfected chTERT RNAs were expressed by the RNA p.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016979
- DOI:
- 10.4238/gmr.15016979