Animal Genetics
Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle
Authors: A.B. Grisolia1,2, G.T. D�Angelo1, L.R. Porto Neto1, F. Siqueira3 andJ.F. Garcia1
The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue .. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr548
- DOI:
- 10.4238/vol8-3gmr548
Neural-endocrine mechanisms of respiratory syncytial virus-associated asthma in a rat model
Authors: Q.G. Li X.R. Wu X.Z. Li J. Yu Y. Xia A.P. Wang J. Wang
We examined the underlying neural-endocrine mechanisms of asthma associated with respiratory syncytial virus infection. Thirty Sprague-Dawley rats were randomly divided into control group, respiratory syncytial virus (RSV) group, and anti-nerve growth factor (NGF) IgG group. An RSV infection model was established by nasal drip once a week. In the anti-NGF.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.3
- DOI:
- 10.4238/2012.August.24.3
Extract from mistletoe, Viscum album L., reduces Hsp27 and 14-3-3 protein expression and induces apoptosis in C6 rat glioma cells
Authors: E.��. U�§ar N. Arda A. Aitken
Extracts of mistletoe (Viscum album) are intensively used in complementary medicine, but their mechanisms are not fully understood in most cases, and the effects on metabolism have not been investigated in detail. However, some biologically active natural products are well known to provoke unexpected cellular responses. They reduce overexpression of heat .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.5
- DOI:
- 10.4238/2012.August.24.5
Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo
Authors: T.M. Raymundo M. Favilla R. Niero S.F. Andrade E.L. Maistro
Plants belonging to the Celastraceae family have been used in traditional medicine for their analgesic, anti-inflammatory and anti-ulcerogenic properties, among others. Maytenus ilicifolia is the principal species of this family, and is used in the treatment of gastric ulcers. However, owing to its inadequate management in Brazil, the species is becoming ext.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.9
- DOI:
- 10.4238/2012.August.24.9
Population genetic analysis and conservation strategies for redtail shrimp Fenneropenaeus penicillatus using ten microsatellite markers
Authors: Y.Y. Cao, J.B. Shangguan, Z.B. Li
Fenneropenaeus penicillatus, which is on the Red List of Endangered Species for China, is an important shrimp species. However, there is not enough genetic information on F. penicillatus for conservation and management purposes. Ten microsatellite markers were used to analyze the genetic diversity, genetic differentiation, and.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019068
- DOI:
- 10.4238/gmr16019068
Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)
Authors: C.Y. Pang T.X. Deng D.S. Tang C.Y. Yang H. Jiang B.Z. Yang X.W. Liang
The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, inclu.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.3
- DOI:
- 10.4238/2012.July.10.3
Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)
Authors: X.J. Chen Z.B. Li L. Chen Y.Y. Cao Q.H. Li
The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.4
- DOI:
- 10.4238/2012.May.22.4
Insight into gene evolution within Cervidae and Bovidae through genetic variation in MHC-DQA in the black muntjac (Muntiacus crinifrons)
Authors: H.-L. Wu C.-C. Tong E. Li T.-L. Luo
The critical role that the major histocompatibility complex plays in the immune recognition of parasites and pathogens makes its evolutionary dynamics exceptionally relevant to ecology, population biology, and conservation studies. The black muntjac is a rare deer endemic to a small mountainous region in eastern China. We found that this species has two D.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.15.13
- DOI:
- 10.4238/2012.May.15.13
Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle
Authors: Y. Yang J.M. Huang Z.H. Ju Q.L. Li L. Zhou R.L. Li J.B. Li F.X. Shi J.F. Zhong C.F. Wang
The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investi.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.12
- DOI:
- 10.4238/2012.May.18.12
Doxycycline-regulated growth hormone gene expression system for swine
Authors: B.C. Jiang D.B. Yu L.J. Wang F.L. Dong H.A. Kaleri X.G. Wang N. Ally J. Li H.L. Liu
Although growth hormone gene transgenic animals are much larger than normal animals, they manifest differences that have adverse effects on survival due to overexpression of growth hormone. We developed a stable pig embryonic fibroblast cell line expressing pig growth hormone (pGH) using the Tet-On system, with which we can conditionally manipulate expressio.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.5
- DOI:
- 10.4238/2012.July.10.5